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Molecular analysis of genetic diversity in natural populations of beech species

山毛榉自然种群遗传多样性的分子分析

基本信息

批准号：
11460149
负责人：
MIKAMI Tetsuo
金额：
$ 3.33万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

The amount and distribution of mitochondrial (mt) DNA restriction fragment length polymorphism was determined among individual tree samples of two Japanese beech species, Fagus crenata and F.japonica. Individual plants were collected from 16 F.crenata populations throughout the range of the species, and from three F.japonica populations. We detected enough variation to characterize eleven and three chondriome types in F.crenata and F.japonica, respectively. The grouping of beech chondriome types based upon the cladistic analysis of mtDNA polymorphism allowed us to recognize the apparent geographical patterns of mtDNA diversity : the resulting three main groups occupied distinct geographic areas. This geographic differentiation is likely to reflect the history of the Japanese beech forests after the last glacial period of the Pleistocene. In addition, the mtDNA polymorphism encountered within F.crenata encompassed all the variation observed in F.japonica. Our result suggests the need for re-evaluation of their phylogenetic relationships.MtDNA restriction fragment length polymorphisms were also used to examine cytoplasmic diversity within a relic-like population of F.hayatae, located in northern Taiwan. The analysis failed to reveal any polymorphisms, an observation that suggests cytoplasmic uniformity in the F.hayatae population examined. It is also interesting to note that the chondriome type of our F.hayatae samples is very close to that characteristic of F.crenata populations in the southernmost area of Japan.

线粒体（mt）DNA限制性片段长度多态性的数量和分布之间的两个日本山毛榉种，毛毛榉和F.japonica的个别树样品的测定。个体植物收集自16个F.crenata种群，遍及该物种的范围，并从3个F.japonica种群。我们检测到足够的变异，以表征11个和3个在F.crenata和F.japonica的染色体组类型分别。山毛榉染色体组类型的分组的基础上的mtDNA多态性的分支分析，使我们能够认识到明显的地理格局的mtDNA多样性：由此产生的三个主要群体占据不同的地理区域。这种地理分化可能反映了更新世末次冰期后日本山毛榉林的历史。此外，线粒体DNA多态性内遇到的F.crenata涵盖了所有的变化观察到的F. japonica。本研究亦利用线粒体DNA限制性片段长度多态性（MtDNA restriction fragment length polymorphisms）分析台湾北方一具残遗样的海鞘群体的细胞质多样性。该分析未能揭示任何多态性，这一观察结果表明所检查的海氏镰刀菌种群中的细胞质均匀性。值得注意的是，我们的F. hayaerae样本的麻烦类型非常接近日本最南端地区的F.crenata种群的特征。