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Studies for different reproductive strategies in Chenopodiaceae crops

藜科作物不同繁殖策略的研究

基本信息

批准号：
21380001
负责人：
MIKAMI Tetsuo
金额：
$ 12.31万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2009
资助国家：
日本
起止时间：
2009 至 2011
项目状态：
已结题

项目摘要

Sugar beet and spinach, members of Chenopodiaceae, have evolved different strategies to prevent self-fertilization and promote outcrossing. The former has developed cytoplasmic male sterility (CMS) and the latter has evolved dioecism. Molecular mechanisms of the CMS and the dioecism in the crops were analyzed to understand the diversity of reproductive strategies in plants.In sugar beet, Owen CMS-associated pre-SATP6 is a mitochondrial membrane protein that assembles into a homogeneous 200-KDa protein complex. Here we found that the product of a nuclear restorer gene (Rf1) for Owen CMS interacts with preSATP6, resulting in the significant reduction of the 200-KDa protein complex and restoration of pollen fertility. We also identified at least 16 distinct alleles in the Rf1 locus from sugar beet and its close relatives, using a variety of DNA markers. The molecular mechanisms of other sources of CMS and evolution of Rf1 locus are worthy of further investigation.Spinach (Spinacia oleracea) is a dioecious plant. Our study revealed that its wild relatives, S. turkestanica and S. tetrandra, are also dioecious plants with unisexual flowers morphologically quite similar to those of spinach. We identified male specific DNA sequences in spinach, which were also found to be male-specific for all accessions of S. turkestanica and some accessions of S. tetrandra examined. The accessions from the wild species were shown to possess chloroplast intergenic sequences (trnL-rpl32) identical to that of spinach (S. oleracea). However, most accessions of S. tetrandra lack the DNA sequences specific for male spinaches, whose trnL-rpl32 sequences are distinctive from that of spinach. The results suggested that the sex determining locus and sex chromosomes are differentiated among members of genus Spinacia.

作为藜科植物的成员，甜菜和菠菜已经进化出不同的策略来防止自花受精和促进异交。前者发生细胞质雄性不育（CMS），后者发生雌雄异株。分析了植物CMS和雌雄异株的分子机制，以了解植物生殖策略的多样性。在甜菜中，Owen cms相关的pre-SATP6是一种线粒体膜蛋白，可组装成均匀的200 kda蛋白复合物。本研究发现，欧文CMS的一个核恢复基因（Rf1）的产物与preSATP6相互作用，导致200-KDa蛋白复合物的显著减少和花粉育性的恢复。我们还使用多种DNA标记在甜菜及其近亲的Rf1位点上鉴定了至少16个不同的等位基因。其他CMS来源的分子机制和Rf1位点的进化值得进一步研究。菠菜（Spinacia oleacea）是一种雌雄异株植物。我们的研究表明，其野生近缘种土耳其蓟马（S. turkestanica）和金蓟马（S. tetrandra）也是雌雄异株植物，花的单性形态与菠菜非常相似。我们在菠菜中鉴定出雄性特异性的DNA序列，这些序列在所有的土耳其斯坦菌株和一些蓟马菌株中也被发现是雄性特异性的。结果表明，野生种的叶绿体基因间序列（trnL-rpl32）与菠菜（S. oleracea）相同。然而，大多数材料缺乏雄性菠菜特有的DNA序列，其trnL-rpl32序列与菠菜不同。结果表明，刺豆属植物的性别决定位点和性染色体在其属成员间存在分化。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The distribution of male sterile cytoplasms in garden and leaf beet germplasm resources.

园林甜菜种质资源中雄性不育细胞质的分布.

DOI：
发表时间：
2010
期刊：
影响因子：
0
作者：
安益公一郎;日渡祐二;小嶋美紀子;榊原均;上田(田中)美祢子;長谷部光泰;and 松岡信;T.Mikami
通讯作者：
T.Mikami

Genetic analysis of the genes for dioecism and monoecism in Spinacia oleracea L

菠菜雌雄异株和雌雄同株基因的遗传分析

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Yasuyuki Onodera;Hiroki Masumo;Itaru Yonaha;Kazuki Yamamoto;Yuji Oda;Tetsuo Mikami
通讯作者：
Tetsuo Mikami

Mapping of the genes for dioecism and monoecism in Spinacia oleracea L.: evidence that both genes are closely linked

DOI：
10.1007/s00299-010-0998-2
发表时间：
2011-02
期刊：
Plant Cell Reports
影响因子：
6.2
作者：
Y. Onodera;Itaru Yonaha;Hiroki Masumo;A. Tanaka;S. Niikura;Seishi Yamazaki;T. Mikami
通讯作者：
Y. Onodera;Itaru Yonaha;Hiroki Masumo;A. Tanaka;S. Niikura;Seishi Yamazaki;T. Mikami

Molecular basis of cytoplasmic male sterility in beets : an overview、Plant Genetic Resources

甜菜细胞质雄性不育的分子基础：概述，植物遗传资源