Genetic and molecular studies on Azolla hybrids and mutants

满江红杂交种和突变体的遗传和分子研究

• 批准号: 06454071
• 负责人: WATANABE Iwao
• 金额: $ 4.61万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454071/
• 关键词: Azolla; A-japonica; N_2-Fixation; DNA; Chewo taxonruy; Genetics; アカウキクサ; A.japonica; 交雑; 突然変異; オオアカウキクサ; PCR

1.Azolla japonicais considered an indigenous species in Japan. The taxonomic position of this species is not well recognized. Because sexual organs, on which taxonomy is principally based, are seldom formed in Azolla, chemical taxonomy was tried by using RAPD (Random Amplification Polymorphic DNA) method.From the work in 1994, at least two types of A.japonica were identified by RAPD ; one is close to A.filiculoides, and another to A.rubra. Takeshi Suzuki, Hyougo Museum of Nature and Human, also noticed two types, based on isozyme analysis. Both researchers agreed to work together, and a large number of the specimen were collected from Japan. RAPD analysis revealed the presence of two types, and coincided with the grouping by isozyme loci, studied by Suzuki.Group closer to A.filiculoides was distributed in Osaka, and Kyoto. Another closer to A.rubra was distributed in wider areas from Kanto to Shikoku Regions.F1 generation was obtained from self-pollinated population of A.filiculoides, Osaka.Isozyme analysis revealed the parent was a heterozygote in terms of estrase loci.

1.满江红被认为是日本的本土物种。本种的分类学位置不被很好地承认。由于满江红很少形成作为分类学基础的性器官，因此，利用RAPD（Random Amplification Polymorphic DNA）技术对满江红进行了化学分类学研究，从1994年开始，利用RAPD技术对满江红进行了至少两种类型的鉴定，一种与细叶满江红相近，另一种与红花满江红相近。兵库县自然与人类博物馆的铃木武也根据同工酶分析注意到了两种类型。两位研究人员同意合作，并从日本收集了大量标本。RAPD分析结果表明，该类群存在两个类型，与Suzuki等的同工酶位点聚类结果相一致，其中大坂和京都分布的类群与丝状放线菌（A.filiculoides）相近。另一个与红曲霉亲缘关系较近的亲本分布在关东至四国地区，F1代来自大坂的丝状曲霉自花授粉群体，同工酶分析表明，亲本在雌激素酶位点上为杂合子。

项目成果

I.Watanabe,C.Von Hove: "phylogenetic,molecular,and breeding aspects of Azolla-Anabaeha symbiosis," Internationla Symposium of Pteridophyte 95,. (印刷中). (1996)

I. Watanabe, C. Von Hove：“满江红-Anabaeha 共生的系统发育、分子和育种方面”，国际蕨类植物研讨会 95（印刷中）。

渡辺厳・川島圭子: "本邦における導入アゾラの生育について" 熱帯農学. 30(印刷中). (1996)

Isamu Watanabe 和 Keiko Kawashima：“论日本引进的满江红的生长”《热带农业》30（出版中）。

I.Watanabe, C.Van Hove: "Phylogenetic, molecular, and breeding aspacts of Azolla-Anabaena symbiosis" International Symposium of Pteridophyte 95. (in press). (1996)

I.Watanabe、C.Van Hove：“满江红-鱼腥藻共生的系统发育、分子和育种问题”国际蕨类植物研讨会 95。（正在出版）。

I. Watanabe, C. Van Hove: "Philogenetic, molecular, and breeding aspects of Azolla‐Anabaena symbiosis." Proc. International Pteridophyte 95. (印刷中). (1996)

I. Watanabe, C. Van Hove：“满江红-鱼腥草共生的系统发育、分子和育种方面。”Proc. International Pteridophyte 95（出版中）。