Genetic and molecular studies on Azolla hybrids and mutants

满江红杂交种和突变体的遗传和分子研究

• 批准号: 06454071
• 负责人: WATANABE Iwao
• 金额: $ 4.61万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454071/
• 关键词: Azolla; A-japonica; N_2-Fixation; DNA; Chewo taxonruy; Genetics; アカウキクサ; A.japonica; 交雑; 突然変異; オオアカウキクサ; PCR

1.Azolla japonicais considered an indigenous species in Japan. The taxonomic position of this species is not well recognized. Because sexual organs, on which taxonomy is principally based, are seldom formed in Azolla, chemical taxonomy was tried by using RAPD (Random Amplification Polymorphic DNA) method.From the work in 1994, at least two types of A.japonica were identified by RAPD ; one is close to A.filiculoides, and another to A.rubra. Takeshi Suzuki, Hyougo Museum of Nature and Human, also noticed two types, based on isozyme analysis. Both researchers agreed to work together, and a large number of the specimen were collected from Japan. RAPD analysis revealed the presence of two types, and coincided with the grouping by isozyme loci, studied by Suzuki.Group closer to A.filiculoides was distributed in Osaka, and Kyoto. Another closer to A.rubra was distributed in wider areas from Kanto to Shikoku Regions.F1 generation was obtained from self-pollinated population of A.filiculoides, Osaka.Isozyme analysis revealed the parent was a heterozygote in terms of estrase loci.

1.azolla japonicais在日本认为是一种土著物种。该物种的分类位置尚未得到很好的认可。由于分类法主要基于基于的性器官很少在Azolla中形成，因此使用RAPD（随机扩增多态性DNA）方法尝试了化学分类法。从1994年的工作中，RAPD至少从1994年的作品确定了两种类型的A.Japonica。一个靠近A.filiculoides，另一个接近A.Rubra。 Hyougo自然与人类博物馆Takeshi Suzuki，基于同工酶分析，也注意到了两种类型。两位研究人员都同意一起工作，并从日本收集了许多标本。 RAPD分析表明存在两种类型，并与铃木的同工酶基因座的分组相吻合，靠近A.filiculoides的组分布在大阪和京都。从关东到Shikoku地区的更广阔的地区，另一个更接近A.rubra的地方。f1产生是从A.filiculoides的自授粉种群中获得的，大阪。异酶分析显示，父母是雌激素基因座的杂合子。

项目成果

I.Watanabe,C.Von Hove: "phylogenetic,molecular,and breeding aspects of Azolla-Anabaeha symbiosis," Internationla Symposium of Pteridophyte 95,. (印刷中). (1996)

I. Watanabe, C. Von Hove：“满江红-Anabaeha 共生的系统发育、分子和育种方面”，国际蕨类植物研讨会 95（印刷中）。

渡辺厳・川島圭子: "本邦における導入アゾラの生育について" 熱帯農学. 30(印刷中). (1996)

Isamu Watanabe 和 Keiko Kawashima：“论日本引进的满江红的生长”《热带农业》30（出版中）。

I. Watanabe, C. Van Hove: "Philogenetic, molecular, and breeding aspects of Azolla‐Anabaena symbiosis." Proc. International Pteridophyte 95. (印刷中). (1996)

I. Watanabe, C. Van Hove：“满江红-鱼腥草共生的系统发育、分子和育种方面。”Proc. International Pteridophyte 95（出版中）。

I.Watanabe, C.Van Hove: "Phylogenetic, molecular, and breeding aspacts of Azolla-Anabaena symbiosis" International Symposium of Pteridophyte 95. (in press). (1996)

I.Watanabe、C.Van Hove：“满江红-鱼腥藻共生的系统发育、分子和育种问题”国际蕨类植物研讨会 95。（正在出版）。