权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on freezing torelance of embryo and larvae of aquatic animals

水产动物胚胎及幼体的冷冻耐受性研究

基本信息

批准号：
06454098
负责人：
KUROKURA Hisashi
金额：
$ 2.88万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

Freezing tolerance of embryo or larvae was compared among aquatic animals. Amictic female egg of rotifer could successfully be cryopreserved in liquid nitrogen and could grow to spawner after thawing Freezing tolerance of the embryo varied during embryonic development. The highest freezing tolerance was observed at symmetrical embryo atage. Trochophore stage larvae obtained from blue mussel also survived after preservation in liquid nitrogen and they reached veliger stage. In case of nauplius larvae of barnacles, activity of survivor in liquid nitrogen was very low and only small portion of survior grew to setting individuals. Dehydration from nauplius larvae during first slow cooling was supposed to be a determinant of survival by second rapid cooling. Any survival could not be observe after cooling to -196ﾟC in flounder and seabream eggs. Formation of auditory vesicle stage embryo and later stage embryo of flounder could survive after cooling to -20ﾟC,when they were thawed just after reaching at the temperature. However, Sea bream embryo at the developmental stage could survie for only 15 min. at -20ﾟC.Conclusively, smaller embryo or larva has higher freezing tolerance than larger animal when compared interspecifically, though freezing tolerance of them are varies during development even in same species. Gene bank by cryopreservation of embryo or larvae seems to be possible in the animal of which size is smaller than 0.3mm.

比较了几种水生动物胚胎或幼体的抗冻性。轮虫无融合生殖的雌卵在液氮中能成功地冷冻保存，解冻后能发育成产卵体。在胚对称发育时期，抗冻性最高。蓝贻贝担轮幼虫经液氮保存后也能存活，并达到面盘幼虫期。藤壶无节幼体在液氮中的存活活力很低，只有一小部分存活幼体能长成结实个体。第一次缓慢冷却期间无节幼体的脱水被认为是第二次快速冷却存活的决定因素。在比目鱼和海鸟卵中，在冷却至-196 ° C后不能观察到任何存活。比目鱼听泡形成期胚胎和后期胚胎在冷却至-20 ℃后仍能存活，而在达到该温度后立即解冻。因此，在种间比较中，较小的胚胎或幼体比较大的胚胎或幼体具有更高的抗冻性，但即使在同一物种中，较小的胚胎或幼体在发育过程中的抗冻性也存在差异。在小于0.3mm的动物中，通过胚胎或幼虫的冷冻保存建立基因库似乎是可能的。