A study of exocrine cell functions using local photolysis of caged compounds.

利用笼状化合物的局部光解研究外分泌细胞功能。

• 批准号: 06454144
• 负责人: KASAI Haruo
• 金额: $ 4.42万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454144/
• 关键词: Calcium; Exocrine gland; Exocytosis; Patch Clamp; Zymogeu granule; Acetylcholine; Caged compounds; カルシウム(Ca); 外分泌腺; イオンチャネル

Agonists evoke Ca^<2+> gradients and oscillations initiating at the trigger zone in exocrine acinar cells. Using a low affinity Ca^<2+> indicator dye, BTC,we found that agonists raised Ca^<2+> concentration as high as 10 muM at the trigger zone. Comparison with the results obtained with fura-2 suggests profuse Ca^<2+> release and Ca^<2+> domains at the trigger zone. The resulting micromolar Ca^<2+> gradients were necessary and sufficient for exocytosis of zymogen granules investigated with the capacitance measurement. In contrast, submicromolar Ca^<2+> gradients selectively gave rise to sequential activation of luminal and basal ion channels. These results emphasize critical role of the micromolar Ca^<2+> gradients in exocytosis, and prompt reinvestigation of other cells using low affinity Ca^<2+> indicators.

激动剂引起外分泌腺泡细胞触发区的Ca^2+梯度和振荡.使用低亲和力的Ca^2+指示剂染料BTC，我们发现激动剂使触发区的Ca^2+浓度升高高达10 μ M。与fura-2的结果相比，提示触发区有大量的Ca^2+释放和Ca^2+结构域。由此产生的微摩尔Ca^2+梯度对于用电容测量法研究的酶原颗粒的胞吐作用是必要和充分的。与此相反，亚微摩尔浓度的Ca^2+梯度选择性地引起腔离子通道和基底离子通道的顺序激活。这些结果强调了微摩尔Ca^2+梯度在胞吐中的关键作用，并促使人们用低亲和力Ca^2+指示剂重新研究其他细胞。

项目成果

Kasai,H.et al.: "Two components of exocytosis and endocytosis in PC12 cells stndied using caged Ca^<2+> compound." J.Physiology. in press.

Kasai,H.et al.：“PC12 细胞中胞吐作用和内吞作用的两个组成部分使用笼状 Ca^2 化合物进行了稳定。”

Kasai. et al.: "Paureats Ca^<2+> waves and secvetion" Celciu waves, gradients and osillation (Ciba Foundation Symposium 188)Wiley, Chichestes. 104-120 (1995)

葛西。

Kasai, H., Takagi, H., Ninomiya, Y., Kishimoto, T., Yosida, A., Yoshioka, T.& Miyashita, Y.: "Two components of exocytosis and endocytosis in PC12 cells studied using caged-Ca^<2+> compounds." J.Physiology. (in press). (1996)

葛西，H.，高木，H.，二宫，Y.，岸本，T.，吉田，A.，吉冈，T.

Ninomiya,et al.: "Ca^<2±> deperdent exocytotic pathmcys in CHD fibroblats renealed by capaeitance measurement and a caged-Ca^<2+> compound" J.Physiology. in press.

Ninomiya等人：“通过电容测量和笼状Ca 2+ 化合物更新的CHD成纤维细胞中Ca 2+ 依赖的胞吐病理”J.Physiology出版。

Terauchi Y. et al.: "Pancreaticβ-cell-specific fargeted disrnption of gluco kinase gene." J. Biol. Chem.270. 30253-30256 (1995)

Terauchi Y. 等人：“葡萄糖激酶基因的胰腺β细胞特异性破坏”，J. Biol. 30253-30256 (1995)。