The mechanism of exocrine gland disorder through estrogen and/or endocrine disruptors
雌激素和/或内分泌干扰物引起外分泌腺疾病的机制
基本信息
- 批准号:16591846
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2004
- 资助国家:日本
- 起止时间:2004 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
To study the possibility of EBY reactivation by estrogen and endocrine disruptors, we analyzed BZLF1 promoter assay and immunoblotting for ZEBRA protein. Estradiol inhibited ZEBRA expression induced by TPA in B95-8 and Akata cells. We established stable B95-8 cell lines which transfected pZp221-Luc and pZp552-Luc. In these cells estradiol also inhibited luciferase activity activated by TPA. In Hela, HSY and HSG cells, estradiol inhibited Zp activity induced by both ZEBRA transfection or TPA stimulation. The effect of estrogen was revealed in 30 minutes, thus we speculated a part of these effects might be a non-genomic event through membrane ER or GPR30. The mechanisms of estrogen on Zp activity are being searched in detail. Next, we analyzed the effects of endocrine disruptors with the same procedure using BPA,3-MC,B[a]P and TCDD. None of these chemicals changed the ZEBRA expression or Zp activity in B94-8 and Akata cells. In contrast to these effects on B cells, Zp activity was enhanced by 3-MC and TCDD in Hela, HSG and HSY cells. When we transfected AhR/Arnt and ER, the activity of Zp was decreased in Hela cells whereas enhanced in HSG and HSY cells compared with AhR/Arnt alone. These results indicated that ER could regulate the Zp activity induced by dioxins depending on the cell types. In HSY cells, although ZEBRA transactivation of Zp was not changed by dioxins without AhR/Arnt, high activity of Zp was observed when ZEBRA and AhR/Arnt were cotransfected. We speculated that dioxins might be an enhancer of EBV reactivation in epithelial cells. To determine the interaction between AhR/Arnt and ZEBRA, we are investigating using immunoprecipitation, GST pull-down assay, Gel shift assay and foot printing.
为了研究雌激素和内分泌干扰物激活Eby的可能性,我们分析了BZLF1启动子和斑马蛋白的免疫印迹。雌二醇抑制TPA诱导的B95-8和Akata细胞中Zebra的表达。建立了稳定表达pZp221-Luc和pZp552-Luc的B95-8细胞系。在这些细胞中,雌二醇也抑制TPA激活的荧光素酶活性。在Hela、HSY和HSG细胞中,雌二醇抑制斑马蛋白转染法和TPA刺激法诱导的ZP活性。雌激素的作用在30分钟内显现,推测部分作用可能是通过膜ER或GPR30发生的非基因组事件。雌激素对ZP活性的影响机制正在探索中。接下来,我们使用BPA、3-MC、B[a]P和TCDD分析了同样程序的内分泌干扰物的影响。这些化学物质都没有改变B94-8和Akata细胞中斑马的表达或ZP活性。3-MC和TCDD可增强Hela、HSG和HSY细胞的ZP活性,而对B细胞的作用则相反。转染AhR/Arnt和ER后,Hela细胞ZP活性降低,HSG和HSY细胞ZP活性增强。这些结果表明,内质网对二恶英诱导的ZP活性的调节作用与细胞类型有关。在HSY细胞中,在没有AhR/Arnt的情况下,二恶英对ZP的反式激活没有影响,但当Zebra和AhR/Arnt共转染时,ZP的活性很高。我们推测,二恶英可能是EBV在上皮细胞中重新激活的增强剂。为了确定AhR/Arnt与斑马的相互作用,我们正在使用免疫沉淀、GST下拉试验、凝胶移位试验和足印等方法进行研究。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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INOUE Hiroko其他文献
Identification of DAMPs in mother’s milk of a cohort study on atopic dermatitis in breastfed infants ; part 1: In vivo study of skin and gut of breastfed HR-1 mice
母乳喂养婴儿特应性皮炎队列研究中 DAMP 的鉴定第 1 部分:母乳喂养 HR-1 小鼠皮肤和肠道的体内研究
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
KONG Weng Sheng;INOUE Hiroko;GUO Yun;SHIMOJO Naoki;KANNO Masamoto - 通讯作者:
KANNO Masamoto
INOUE Hiroko的其他文献
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{{ truncateString('INOUE Hiroko', 18)}}的其他基金
Mechanism of EBV induced salivary gland disorder via TLR
EBV通过TLR诱导唾液腺疾病的机制
- 批准号:
21592347 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research of biomarker to reflect the dietary habit and nutritional status of adolescence and development of nutrition education program.
反映青春期饮食习惯和营养状况的生物标志物研究及营养教育方案的制定。
- 批准号:
21700762 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Pathogenesis of Epstein-Barr virus on tissue specific autoimmune disease in humanized mice
EB 病毒对人源化小鼠组织特异性自身免疫性疾病的发病机制
- 批准号:
19592137 - 财政年份:2007
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Clarification of mechanism of cell degeneration using Drosophila and mouse mutant
利用果蝇和小鼠突变体阐明细胞变性机制
- 批准号:
02680205 - 财政年份:1990
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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