Investigation of Ca-dependent cellular functions with caged compounds
用笼状化合物研究 Ca 依赖性细胞功能
基本信息
- 批准号:12470006
- 负责人:
- 金额:$ 8.38万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Dendritic spines serve as preferential sites of excitatory synaptic connections and are pleomorphic. To address the structure- function relationship of the dendritic spines, we used two-photon uncaging of glutamate to allow mapping of functional glutamate receptors at the level of the single synapse. Our analyses of the spines of CA1 pyramidal neurons reveal that AMPA (α-amino-3-hydroxyl-5-methyl-4-isoxazolep ropionic acid)-type glutamate receptors are abundant (up to 150/spine) in mushroom spines but sparsely distributed in thin spines and filopodia. The latter may be serving as the structural substrates of the silent synapses that have been proposed to play roles in development and plasticity of synaptic transmission. Our data indicate that distribution of functional AMPA receptors is tightly correlated with spine geometry and that receptor activity is independently regulated at the level of single spines.Insulin secretion from intact mouse pancreatic islets was investigated with two-photon excitation imaging. Insulin granule exocytosis occurred mainly toward the interstitial space, away from blood vessels. The fusion pore was unusually stable with a lifetime of 1.8 seconds. Opening of the 1.4-nanometerdiameter pore was preceded by unrestricted lateral diffusion of lipids along the inner wall of the pore, supporting the idea that this structure is composed of membrane lipids. When the pore dilated to 12 nanometers, the granules rapidly attened and discharged their contents. Thus, our methodology reveals fusion pore dynamics in intact tissues at nanometer resolution.
树突棘是兴奋性突触连接的优先位点,并且是多形性的。为了解决树突棘的结构-功能关系,我们使用谷氨酸的双光子解禁来允许在单个突触水平上绘制功能性谷氨酸受体。我们对 CA1 锥体神经元棘的分析表明,AMPA(α-氨基-3-羟基-5-甲基-4-异恶唑丙酸)型谷氨酸受体在蘑菇棘中丰富(高达 150 个/棘),但稀疏分布在细棘和丝状伪足中。后者可能作为沉默突触的结构基质,已被认为在突触传递的发育和可塑性中发挥作用。我们的数据表明,功能性 AMPA 受体的分布与脊柱几何形状密切相关,并且受体活性在单个脊柱水平上受到独立调节。通过双光子激发成像研究了完整小鼠胰岛的胰岛素分泌。胰岛素颗粒胞吐作用主要发生在远离血管的间质空间。融合孔异常稳定,寿命为 1.8 秒。在 1.4 纳米直径的孔打开之前,脂质沿着孔内壁不受限制地横向扩散,这支持了该结构由膜脂组成的观点。当孔扩大到12纳米时,颗粒迅速聚集并排出其内容物。因此,我们的方法揭示了完整组织中纳米分辨率的融合孔动力学。
项目成果
期刊论文数量(56)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kishimoto, T.: "Ion selectivities of the Ca2+ sensors for exocytosis in rat phaeochromocytoma cells"J. Physiol. (Lond.). 533. 627-637 (2001)
Kishimoto, T.:“Ca2 传感器对大鼠嗜铬细胞瘤细胞胞吐作用的离子选择性”J。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Kasai, H.: "Fast and cAMP-sensitive mode of Ca^<2+>-dependent exocytosis in pancreatic β cells"Diabetes. 51. S19-S24 (2002)
Kasai,H.:“胰腺β细胞中Ca 2+ 依赖性胞吐作用的快速和cAMP敏感模式”51.S19-S24(2002)。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Takahashi, N.: "Two-photon excitation imaging of pancreatic islets with various fluorescent probes"Diabetes. 51(S1). S25-S28 (2002)
Takahashi, N.:“用各种荧光探针对胰岛进行双光子激发成像”糖尿病。
- DOI:
- 发表时间:
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- 影响因子:0
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Kasai, H.: "Structure-stability-function relationships of dendritic spines"Trends in Neurosciences. (in press).
Kasai, H.:“树突棘的结构-稳定性-功能关系”神经科学趋势。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kishimoto,T.: "Ion selectivities of the Ca^<2+> sensors for exocytosis in rat phaeochromocytoma cells"J.Physiol.(Lond.). (in press).
Kishimoto,T.:“大鼠嗜铬细胞瘤细胞胞吐作用的Ca 2+ 传感器的离子选择性”J.Physiol.(Lond.)。
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KASAI Haruo其他文献
KASAI Haruo的其他文献
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{{ truncateString('KASAI Haruo', 18)}}的其他基金
Cerebral spine synapses and exocytosis studied with two-photon microscope
用双光子显微镜研究大脑脊柱突触和胞吐作用
- 批准号:
21000009 - 财政年份:2009
- 资助金额:
$ 8.38万 - 项目类别:
Grant-in-Aid for Specially Promoted Research
A study of exocrine cell functions using local photolysis of caged compounds.
利用笼状化合物的局部光解研究外分泌细胞功能。
- 批准号:
06454144 - 财政年份:1994
- 资助金额:
$ 8.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Development of coaxial-slot antenna for interstitial microwave hyperthermia
间质微波热疗用同轴缝隙天线的研制
- 批准号:
06452446 - 财政年份:1994
- 资助金额:
$ 8.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Development of Znse Blue Light Emitting Devices by Metalorganic Molecular Beam Epitaxy(MOMBE)
金属有机分子束外延(MOMBE)开发Znse蓝光发光器件
- 批准号:
60460060 - 财政年份:1985
- 资助金额:
$ 8.38万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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