Molecular and cell biological investigation about the healing mechanism of gastric mucosal damage.

胃粘膜损伤愈合机制的分子和细胞生物学研究。

• 批准号: 06454266
• 负责人: SATO Nobuhiro
• 金额: $ 4.67万
• 依托单位: Juntendo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454266/
• 关键词: gastric ulcer; growth factor; cytoskeleton; migration; proliferation; wound healing; culture cell; gastric ulcer; growth factor; cytoskeleton; migration; proliferation; wound healing; culture cell; gastric ulcer 男性度; growth factor 増殖因子; cy

The precise cellular mechanism of growth factors on gastric wound repair is still controversial. We investigated the role of growth factors on gastric mucosal restoration using primary cultured rabbit gastric epithelial cells and fibroblasts. Isolated rabbit gastric epitherial cells and fibroblasts were prepared from identical rabbit and cultured in F-12 medium. Inoculated epithelial cells (92% mucous cell) formed complete monolayr in 48h and fibroblasts formed complete monolayr in 4 weeks. A wound with cell-freearea of constant size was created by mechanical cell denudation using rotating silicon tip. The restoration process was monitored by measuring wound size every 12 h using a time-lapse video system. The proliferative cells were detected by serial staining for BrdU.Effects of EGF, Insulin, bFGF, HGF, TGFalpha and PDGF were assessed. EGF, Insulin, HGF, TGFalpha and PDGF accelerated the gastric epithelial restoration with the promotion of cell migration and proliferation. bFGF and PDGF accelerated the gastric mesenchymal restoration. The difference of target cells of growth factor might modulate gastric ulcer healing and the integrity of growth factor network during the process of ulcer healing is essential for high quality ulcer healing.

生长因子对胃伤口修复的精确细胞机制仍存在争议。我们使用原代培养的兔胃上皮细胞和成纤维细胞研究了生长因子对胃粘膜修复的作用。从相同的兔子中制备分离的兔胃上皮细胞和成纤维细胞，并在F-12培养基中培养。接种的上皮细胞(92%粘液细胞)在48小时内形成完整的单层，成纤维细胞在4周内形成完整的单层。使用旋转硅尖端通过机械细胞剥脱产生具有恒定大小的无细胞区域的伤口。通过使用延时视频系统每 12 小时测量一次伤口大小来监测修复过程。通过BrdU连续染色检测增殖细胞。评估EGF、胰岛素、bFGF、HGF、TGFα和PDGF的作用。 EGF、胰岛素、HGF、TGFα和PDGF通过促进细胞迁移和增殖加速胃上皮修复。 bFGF和PDGF加速胃间质修复。生长因子靶细胞的差异可能会调节胃溃疡的愈合，溃疡愈合过程中生长因子网络的完整性对于高质量的溃疡愈合至关重要。

项目成果

Endo K,Watanabe S,Nagahara A,Sato N.: "Restoration of gap junctions in the regenerative process of ethanol induced gastric mucosal injury." J of Gastroenterology and Hepatology. 10. 589-594 (1995)

Endo K，Watanabe S，Nagahara A，Sato N.：“乙醇引起的胃粘膜损伤再生过程中间隙连接的恢复。”

Nobuhiro Sato: "Effect of nicotine in migration and proliferation of rabbit gastric mucosal cells in a culture cell model." J Gastroenterol Hepatology. 9. S66-S71 (1994)

Nobuhiro Sato：“尼古丁对培养细胞模型中兔胃粘膜细胞迁移和增殖的影响。”

佐藤信紘: "日本消化性潰瘍学" 日本消化看病学会附属研究会編(医科学出版社)(in press), (1995)

佐藤信宏：《日本消化性溃疡病学》日本消化道护理学会研究组编（Igical Science出版社）（正在出版），（1995年）

Watanabe S,Hirose M,Maehiro K,Sato N: "A new model to study repair of gastric mucosa using primary cultured rabbit gastric epithelial cells." J Clinical Gastroenterol. 21. 40-44 (1995)

Watanabe S、Hirose M、Maehiro K、Sato N：“利用原代培养的兔胃上皮细胞研究胃粘膜修复的新模型。”

Watanabe S, Wang X, Kitamura T, Sato N: "Basic fibroblast growth factor accelerates gastric mucosal restoration in vitro by promoting mesenchymal cell migration and proliferation." J of Gastroenterology and Hepatology. 10. 627-632 (1996)

Watanabe S、Wang X、Kitamura T、Sato N：“碱性成纤维细胞生长因子通过促进间充质细胞迁移和增殖来加速胃粘膜的体外修复。”