Molecular and cell biological investigation about the healing mechanism of gastric mucosal damage.

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The precise cellular mechanism of growth factors on gastric wound repair is still controversial. We investigated the role of growth factors on gastric mucosal restoration using primary cultured rabbit gastric epithelial cells and fibroblasts. Isolated rabbit gastric epitherial cells and fibroblasts were prepared from identical rabbit and cultured in F-12 medium. Inoculated epithelial cells (92% mucous cell) formed complete monolayr in 48h and fibroblasts formed complete monolayr in 4 weeks. A wound with cell-freearea of constant size was created by mechanical cell denudation using rotating silicon tip. The restoration process was monitored by measuring wound size every 12 h using a time-lapse video system. The proliferative cells were detected by serial staining for BrdU.Effects of EGF, Insulin, bFGF, HGF, TGFalpha and PDGF were assessed. EGF, Insulin, HGF, TGFalpha and PDGF accelerated the gastric epithelial restoration with the promotion of cell migration and proliferation. bFGF and PDGF accelerated the gastric mesenchymal restoration. The difference of target cells of growth factor might modulate gastric ulcer healing and the integrity of growth factor network during the process of ulcer healing is essential for high quality ulcer healing.