The analysis of physical stimulation to proliferative gastric cells.

物理刺激对胃细胞增殖的分析。

• 批准号: 08457175
• 负责人: SATO Nobuhiro
• 金额: $ 4.86万
• 依托单位: JUNTENDO UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457175/
• 关键词: migration; proliferation; apotosis; Helicobacter pylori; bile acid; alcohol; radical; wound healing; Apoptosis

The wound repair of gastric mucosal cells in vitro (i.e.the ability of cultured cells to fill the artificially made cell-free area) requires activation of both cell migration and proliferation.However, in this model, the role of cell migration is much more important than that of proliferation because more than three-fourths of wound repair occurred before detection of BrdU-positive cells. Most of the process, especially the initial stage of repair, is completed by only cell migration, and only the late stage might include cell proliferation. Factors which might modulate the healing process have been investigated. In a series of experiments, we examined the effects of a variety of available growth factors. Addition of growth factors such as hepatocyte growth factor induced wound repair in the monolayr gastric epithelial cell sheet. In this project, we assessed the effects of temperature, free radicals and Helicobacter pylori water extract on the process of restoration using above wound repair model. Free radical inducer such as bile acids, ethanol and H.pylori extract retarded the restoration process including cell proliferation and migration with the induction of apoptosis. Especially, toxin in H.pylori water extract used in this experiment is different from vacA gene products and cagA gene products. Further study must be done to identify the character of this new toxin.

体外胃粘膜细胞的创伤修复（即培养细胞填充人工制造的无细胞区的能力）需要激活细胞迁移和增殖。然而，在该模型中，细胞迁移的作用比增殖重要得多，因为超过四分之三的创伤修复发生在检测到BrdU阳性细胞之前。大部分过程，特别是修复的初始阶段，仅通过细胞迁移完成，并且仅晚期阶段可能包括细胞增殖。可能调节愈合过程的因素已被研究。在一系列实验中，我们研究了各种可用的生长因子的影响。添加生长因子如肝细胞生长因子诱导单层胃上皮细胞片层的损伤修复。本课题利用上述创伤修复模型，研究了温度、自由基和幽门螺杆菌水提取物对修复过程的影响。自由基诱导剂如胆汁酸、乙醇、幽门螺杆菌提取物等可抑制细胞的增殖和迁移，诱导细胞凋亡。特别是，本实验中使用的幽门螺杆菌水提取物中的毒素不同于vacA基因产物和cagA基因产物。必须做进一步的研究来鉴定这种新毒素的特性。

项目成果

Iwazaki R, Watanabe S, et al.: "The role of cytoskeleton in migration and proliferation of a cultured human gastric cancer cell line using a new metastasis model." Cancer Lett 119. 119. 191-199 (1997)

Iwazaki R、Watanabe S 等人：“使用新的转移模型研究细胞骨架在培养的人胃癌细胞系迁移和增殖中的作用。”

Wang XE,Watanabe S,Hirose M,Miyazaki A,Sato N.: "Sucralfate prevents bile acid-induced retardation of gastric epithelial repair in a rabbit cultured cell model." Current Therap Res. 58. 533-543 (1997)

Wang XE、Watanabe S、Hirose M、Miyazaki A、Sato N.：“硫糖铝可防止兔培养细胞模型中胆汁酸诱导的胃上皮修复迟缓。”

Miwa H,Wada R,Nagahara A,Endo K,Watanabe S,Sato N.: "A dislocated and enlarged proliferative zone in human gastric intestinal metaplasia." Cancer Lett. 103. 33-39 (1996)

Miwa H，Wada R，Nagahara A，Endo K，Watanabe S，Sato N.：“人胃肠化生中脱位和扩大的增殖区。”

Watanabe S,Wang XE,Hirose M,Oide H,Kitamura T,Miwa H,Miyazaki A,Sato N.: "Platelet-derived growth factor accelerates gastric epithelial restoration in a rabbit cultured cell model." Gastroenterol. 110. 775-779 (1996)

Watanabe S、Wang XE、Hirose M、Oide H、Kitamura T、Miwa H、Miyazaki A、Sato N.：“血小板衍生生长因子在兔培养细胞模型中加速胃上皮修复。”

Iwazaki R, Watanabe S,et al.: "The role of cytoskeleton in migration and proliferation of a cultured human gastric cancer cell line using a new metastasis model." Cancer Lett. 119. 191-199 (1997)

Iwazaki R、Watanabe S 等人：“使用新的转移模型研究细胞骨架在培养的人胃癌细胞系迁移和增殖中的作用。”