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Analysis of the mechanisms for cytotoxic effects in the perforin/granzyme B system

Analysis of the mechanisms for cytotoxic effects in the perforin/granzyme B system
穿孔素/颗粒酶B系统细胞毒作用机制分析
批准号:
14571221
负责人:
SATO Nobuhiro
金额:
$2.24万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2002
资助国家:
日本
项目状态:
已结题
起止时间:
2002 至 2003

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英文摘要
We developed a new assay for cytotoxic effects of perforin/ granzyme B systems, by using Bio Sensor Chip. The assay utilized the surface plasmon resonance technology for the analysis Biomolecular interaction analysis. The assay is rapid and sensitive method, and moreover, allows precise quantitative membranous injury. We also examined inhibitory effects of seine protease inhibitor (Serpin) family to cellular injury. Maspin is a candidate for intra cellular molecule to inhibit granzyme B system. We are now examining for the inhibitor effects by using the RNAi method.
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Oyama M, Terashima M, Takagane A, Maesawa C.: "Prognostic significance of peritoneal minimal residual disease in detecting mRNA in gastric cancer using quantitative real-time reverse transcriotive-polymerase chain reaction of carcinoembryonic antigen."Br
Oyama M、Terashima M、Takagane A、Maesawa C.:“使用癌胚抗原的定量实时逆转录聚合酶链反应检测胃癌 mRNA 时腹膜微小残留病的预后意义。”Br
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Akiyama Y, Maesawa C, Wada K, Fujisawa K, Itabashi T, Noda Y, Honda T, Sato N, Ishida K, Takagane A, Saito K, Masuda T.: "Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma."Oncol Re. 11(4). 871-874 (2004
Akiyama Y、Maesawa C、Wada K、Fujisawa K、Itabashi T、Noda Y、Honda T、Sato N、Ishida K、Takagane A、Saito K、Masuda T.:“Human PinX1 是一种有效的端粒酶抑制剂,不参与
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Iijima S, Maesawa C, Sato N, Ikeda K, Inaba T, Akiyama Y, Ishida K, Saito K, Masuda T.: "Gastrointestinal stromal tumour of the oesophagus : significance of immunohistochemical and genetic analyses of the c-kit gene."Gastroenterol Hepatol.. 14(4). 445-448
Iijima S、Maesawa C、Sato N、Ikeda K、Inaba T、Akiyama Y、Ishida K、Saito K、Masuda T.:“食管胃肠间质瘤:c-kit 基因免疫组织化学和遗传分析的意义。”
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Nakatani K, Hagihara S, Sando S, Sakamoto S, Yamaguchi K, Maesawa C, Saito I.: "Induction of a remarkable conformational change in a human telomeric sequence by the binding of naphthyridine dimer : inhibition of the elongation of a telomeric repeat by tel
Nakatani K、Hagihara S、Sando S、Sakamoto S、Yamaguchi K、Maesawa C、Saito I.:“通过萘啶二聚体的结合诱导人类端粒序列显着的构象变化:通过抑制端粒重复的延长
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38
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