Analysis of the mechanisms for cytotoxic effects in the perforin/granzyme B system

穿孔素/颗粒酶B系统细胞毒作用机制分析

• 批准号: 14571221
• 负责人: SATO Nobuhiro
• 金额: $ 2.24万
• 依托单位: Iwate Medical University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571221/
• 关键词: perform; granzyme B; bioineratction analysis; maspin; sensor chip; 生体内分子; パーフォリン; CTL; リン脂質膜; リ脂質膜

We developed a new assay for cytotoxic effects of perforin/ granzyme B systems, by using Bio Sensor Chip. The assay utilized the surface plasmon resonance technology for the analysis Biomolecular interaction analysis. The assay is rapid and sensitive method, and moreover, allows precise quantitative membranous injury. We also examined inhibitory effects of seine protease inhibitor (Serpin) family to cellular injury. Maspin is a candidate for intra cellular molecule to inhibit granzyme B system. We are now examining for the inhibitor effects by using the RNAi method.

我们建立了一种新的检测穿孔素/颗粒酶B系统细胞毒效应的方法。该测定利用表面等离子体共振技术进行分析生物分子相互作用分析。该测定是快速和灵敏的方法，而且，允许精确定量膜损伤。我们还研究了丝氨酸蛋白酶抑制剂（Serpin）家族对细胞损伤的抑制作用。Maspin是抑制颗粒酶B系统的细胞内分子的候选者。我们现在正在通过使用RNAi方法来检查抑制剂效果。

项目成果

Oyama M, Terashima M, Takagane A, Maesawa C.: "Prognostic significance of peritoneal minimal residual disease in detecting mRNA in gastric cancer using quantitative real-time reverse transcriotive-polymerase chain reaction of carcinoembryonic antigen."Br

Oyama M、Terashima M、Takagane A、Maesawa C.：“使用癌胚抗原的定量实时逆转录聚合酶链反应检测胃癌 mRNA 时腹膜微小残留病的预后意义。”Br

Akiyama Y, Maesawa C, Wada K, Fujisawa K, Itabashi T, Noda Y, Honda T, Sato N, Ishida K, Takagane A, Saito K, Masuda T.: "Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma."Oncol Re. 11(4). 871-874 (2004

Akiyama Y、Maesawa C、Wada K、Fujisawa K、Itabashi T、Noda Y、Honda T、Sato N、Ishida K、Takagane A、Saito K、Masuda T.：“Human PinX1 是一种有效的端粒酶抑制剂，不参与

Iijima S, Maesawa C, Sato N, Ikeda K, Inaba T, Akiyama Y, Ishida K, Saito K, Masuda T.: "Gastrointestinal stromal tumour of the oesophagus : significance of immunohistochemical and genetic analyses of the c-kit gene."Gastroenterol Hepatol.. 14(4). 445-448

Iijima S、Maesawa C、Sato N、Ikeda K、Inaba T、Akiyama Y、Ishida K、Saito K、Masuda T.：“食管胃肠间质瘤：c-kit 基因免疫组织化学和遗传分析的意义。”

Nakatani K, Hagihara S, Sando S, Sakamoto S, Yamaguchi K, Maesawa C, Saito I.: "Induction of a remarkable conformational change in a human telomeric sequence by the binding of naphthyridine dimer : inhibition of the elongation of a telomeric repeat by tel

Nakatani K、Hagihara S、Sando S、Sakamoto S、Yamaguchi K、Maesawa C、Saito I.：“通过萘啶二聚体的结合诱导人类端粒序列显着的构象变化：通过抑制端粒重复的延长

Uchiyama M, Maesawa C, Yashima A, Itabashi T, Ishida Y Masuda T.: "Consensus primers for detecting monoclonal immunoglobulin heavy chain rearrangement in B-cell lymphomas."J Chin Pathol.. 56(10). 778-779 (2003)

Uchiyama M、Maesawa C、Yashima A、Itabashi T、Ishida Y Masuda T.：“用于检测 B 细胞淋巴瘤中单克隆免疫球蛋白重链重排的共有引物。”J Chin Pathol.. 56(10)。