The establishment of a new prenatal diagnostic method using fetal nucleated cells in maternal peripheral blood

本研究旨在建立一种利用孕妇外周血中胎儿有核细胞进行产前诊断的新方法。研究结果显示了以下几个新的发现：1。正常妊娠和分娩后，孕妇外周血中的胎儿有核细胞频率随时间而降低。这些细胞在大多数（如果不是全部的话）母亲分娩后3个月从母体循环中消失。大多数（如果不是全部的话）母胎出血病例在分娩后3个月，母胎外周血中的胎儿有核细胞检测不到，与正常妊娠和分娩的病例相同.利用孕妇外周血中的胎儿有核细胞与FISH技术进行胎儿性别鉴定是可能的。然而，考虑到分析大量细胞所需的时间和劳动力，在通过该方法进行分析之前需要富集胎儿细胞。4.应用荧光激活细胞分选技术富集孕妇外周血中胎儿有核细胞是可行的。该诊断方法有待进一步完善，以应用于临床。

The purpose of this study was to establish a new prenatal diagnostic method using fetal nucleated cells in maternal peripheral blood. The results of the study showed several new findings as follows ;1. Fetal nucleated cells in maternal peripheral blood decrease in frequency with time after normal pregnancy and delivery. These cells disappear from maternal circulation 3 months after delivery in most, if not all, mothers.2. Fetal nucleated cells in maternal peripheral blood become undetectable 3 months after delivery in most, if not all, cases of fetomaternal hemorrhage the same as in cases of normal pregnancy and delivery.3. Fetal sex determination using fetal nucleated cells in maternal peripheral blood with FISH is possible. However, considering the time and labor required for the analysis of a much larger number of cells, the enrichment of fetal cells is needed before an analysis by this method.4. The enrichment of fetal nucleated cells in maternal peripheral blood by fluorescence activated cell sortings is possible. Further improvement of this diagnostic method is needed to use in clinical medicine.