The establishment of a new prenatal diagnostic method using fetal nucleated cells in maternal peripheral blood

利用母体外周血胎儿有核细胞建立产前诊断新方法

• 批准号: 06454464
• 负责人: KUBO Takeshi
• 金额: $ 4.29万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454464/
• 关键词: Prenatal diagnosis; maternal blood; fetal nucleated cells

The purpose of this study was to establish a new prenatal diagnostic method using fetal nucleated cells in maternal peripheral blood. The results of the study showed several new findings as follows ;1. Fetal nucleated cells in maternal peripheral blood decrease in frequency with time after normal pregnancy and delivery. These cells disappear from maternal circulation 3 months after delivery in most, if not all, mothers.2. Fetal nucleated cells in maternal peripheral blood become undetectable 3 months after delivery in most, if not all, cases of fetomaternal hemorrhage the same as in cases of normal pregnancy and delivery.3. Fetal sex determination using fetal nucleated cells in maternal peripheral blood with FISH is possible. However, considering the time and labor required for the analysis of a much larger number of cells, the enrichment of fetal cells is needed before an analysis by this method.4. The enrichment of fetal nucleated cells in maternal peripheral blood by fluorescence activated cell sortings is possible. Further improvement of this diagnostic method is needed to use in clinical medicine.

本研究旨在建立一种利用孕妇外周血中胎儿有核细胞进行产前诊断的新方法。研究结果如下：1.孕妇外周血中胎儿有核细胞的出现频率在正常妊娠和分娩后随时间的延长而降低。在大多数(如果不是全部)母亲中，这些细胞在分娩后3个月从母亲的血液循环中消失。孕妇外周血中的胎儿有核细胞在分娩后3个月内检测不到，大多数(如果不是全部)胎儿母体出血病例与正常妊娠和分娩的病例相同。利用孕妇外周血中的胎儿有核细胞结合FISH进行胎儿性别鉴定是可能的。然而，考虑到分析更多数量的细胞所需的时间和人力，在用这种方法进行分析之前，需要对胎儿细胞进行浓缩。利用荧光激活细胞分选技术对孕妇外周血中的胎儿有核细胞进行浓缩是可能的。该诊断方法尚需进一步完善，以应用于临床医学。

项目成果

Hiromi Hamada: "Mid-trimester fetal sex determination from maternal peripheral blood by fluorescence in situ hybridization without enrichment of fetal cells" Prenatal Diagnosis. 15. 78-81 (1995)

Hiromi Hamada：“通过荧光原位杂交从母体外周血中确定妊娠中期胎儿性别，无需富集胎儿细胞”产前诊断。

Hiromi Hamada: "Fetal nucleated cells in maternal peripheral blood after delivery" American Journal of Obstetrics and Gynecology. 170. 1188-1193 (1994)

Hiromi Hamada：“分娩后母体外周血中的胎儿有核细胞”美国妇产科杂志。

Hiromi Hamada: "Fetal nucleated cells in maternal peripheral blood after delivery in cases of fetomaternal hemorrhage" Obstetrics and Gynecology. 85. 449-451 (1995)

滨田宏美：“胎母出血病例中分娩后母体外周血中的胎儿有核细胞”妇产科。

Hiromi Hamada: "Fetal nucleated cells in maternal peripheral blood after delivery in cases of fetomaternal hemorrhage" Obstetrics and Gynecology. 85. 449-451 (1994)

滨田宏美：“胎母出血病例中分娩后母体外周血中的胎儿有核细胞”妇产科。

Hiromi Hamada: "Mid-trimester fetal sex determination from maternal peripheral blood by fluorescence in situ hvbridization without enrichment of fetal cells" Prenatal Diagnosis. (発表予定).

Hiromi Hamada：“通过荧光原位杂交从母体外周血中确定妊娠中期胎儿性别，无需富集胎儿细胞”产前诊断（待提交）。