Changes in neuronal structure after auditory deprivation in rat cochlea.

大鼠耳蜗听觉剥夺后神经元结构的变化。

• 批准号: 09470371
• 负责人: KUBO Takeshi
• 金额: $ 2.37万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470371/
• 关键词: congenital deaf; Kanamycin; neuronal cell count; AMPA receptor; NMDA receptor; neurotrophic factor; cochlear implant; 人工内耳動物モデル; グルタミン酸レセプター; 内耳コルチ器; 蝸牛神経核; 肝細胞成長因子; 動物モデル; グルタミン酸; レセプター; 可塑的変化; 上オリーブ核

Rats were deprivated the auditory function by administrating the Kanamycin for two weeks, which started immediately after the birth. Auditory brainstem response evaluated after 2 weeks of birth, showed no response. i) Immunohistochemical study confirmed no hair cells in the cochlea., decrease of neuronal cells in the inner ear and cochlear nucleus. ii) Expression of glutamate receptors were examined by both immunohistochemical study and by quantitative PCR technique. In control animals, glutamate receptors of Glu-R 2-5 (Kainic acid receptors) and NR 1 and 2 (NMDA receptors) were found in spiral ganglion cells and cochlear nucleus neurons. In deaf animals, there was a decrease in Glu-R 4 and NR 1, but a significant increase was found in Glu-R 2/3 and NR 2. The fact that the up-regulation of Glu-R 2/3 and NR 2 occurred in deafned rats reflected functional and structual changes in the cochlea. iii) Neurotrophic factor (HGF) was administered with chronocally embedded miniature pomp in deafned rats, and its protective effects was examined. The reslut showed that HGF had positive effects upon the number of spiral ganglion cells and expression in glutamate receptors. The importance of neurotrophic stimulation upon auditory system of deafned rats was stressed.

老鼠出生后立即开始服用卡那霉素两周，从而剥夺其听觉功能。出生两周后评估听觉脑干反应，显示没有反应。 i) 免疫组织化学研究证实耳蜗中没有毛细胞，内耳和耳蜗核中的神经元细胞减少。 ii)通过免疫组织化学研究和定量PCR技术检查谷氨酸受体的表达。在对照动物中，在螺旋神经节细胞和耳蜗核神经元中发现了 Glu-R 2-5（红藻氨酸受体）和 NR 1 和 2（NMDA 受体）的谷氨酸受体。在耳聋动物中，Glu-R 4 和 NR 1 下降，但 Glu-R 2/3 和 NR 2 显着增加。耳聋大鼠中 Glu-R 2/3 和 NR 2 上调的事实反映了耳蜗功能和结构的变化。 iii) 神经营养因子（HGF）在耳聋大鼠体内以时间嵌入的微型浮点给药，并检查其保护作用。结果表明，HGF对螺旋神经节细胞的数量和谷氨酸受体的表达有积极的影响。强调了神经营养刺激对耳聋大鼠听觉系统的重要性。

项目成果

Okusa M, Shiraishi T, Kubo T, Nageishi Y.: "Effects of discrimination difficulty on cognitive event-related brain potentials in patients with cochlear implants."Otolaryngol Head Neck Surg. 121. 610-615 (1999)

Okusa M、Shiraishi T、Kubo T、Nageishi Y.：“辨别困难对人工耳蜗植入患者认知事件相关大脑电位的影响。”耳鼻喉头颈外科。

久保武,山本好一: "内耳電気刺激に対する聴覚の可塑性."音声言語医学. 39. 329-333 (1998)

Takeshi Kubo，Koichi Yamamoto：“内耳电刺激的听觉可塑性”，39. 329-333 (1998)。

井脇貴子, 土井勝美, 久保武: "MPEAKコード化法とSPEAKコード化法間における言語聴取能の比較" Audiolpgy Japan. 40. 72-77 (1997)

Takako Iwaki、Katsumi Doi、Takeshi Kubo：“MPEAK 编码方法与 SPEAK 编码方法之间的语言听力能力比较”Audilpgy Japan 40. 72-77 (1997)。

Fujii K, Saika T, Yamamoto K, Doi K, Kubo T: "Advances in Oto-Rhino-Laryngology" S.Karger,Basel., 3 (1997)

Fujii K、Saika T、Yamamoto K、Doi K、Kubo T：“耳鼻喉科进展”S.Karger，巴塞尔，3 (1997)

Kubo T, Nishimura H, 他: "Sign language heard in the auditory cortex" Nature. 397. 116-116 (1999)

Kubo T、Nishimura H 等人：“听觉皮层中听到的手语”《自然》397. 116-116 (1999)。