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On the calcification of cartilage and tooth germ in vitro.

体外软骨和牙胚钙化的研究。

基本信息

批准号：
06454513
负责人：
NAWA Tokio
金额：
$ 3.71万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454513/
关键词：
Tissue culture Tooth germ Cartilage Mechel's cartilage Calcification Meckel' s cartilage

项目摘要

(1) On the enamel-free area : At the cusp tip of rodent molar, there is a region of dentin without an enamelcap which is colled enamel-free area (AFE). To clarify the cause of enamel-free area, we observed the ultrastructure of the basement membrane and the distal ends of the inner enamel epithelium in rats. On rats. On the dentin covered with enamel (DCE), collagen fibrils were arranged perpendicular to the basal lamina. On EFA,collagen fibrils ran parallel to the basal lamina. These finding suggested that the differences in the collagen arrangement between the EFA and DCE are associated with the developmental state of aperiodic microfibrils in the basal lamina. (2) On the osteogenic cell transformation of Meckel's cartilage chondrocytes : The Meckel's cartilage is a fetal organ with a resorptive ultimate fate soon after birth. However, we showed that when Meckel's cartilage was maintained in heterotopic site or cell culture, it expressed bone-type cellular element and matrix calcification. From these results in vivo and in vitro, it is clear that the cellular microenvironment such as extracellular matrix, cellular multiplication and blood promote the phenotypic conversion of Meckel's cartilage cells into osteogenic cells.(3) On the development of the cellular cementum by the new organ culture method : We report a new organ culture method by which nearly normal development of root formation can be obtained. By this culture method the three-dimensional organogenesis and epithelial-mesenchymal interactions could be maintained.In this study, we showed for the first time the formation of cellular cementum in a molar organ system in vitro.

(1)无釉区：在磨牙牙尖部，有一个牙本质区没有釉帽，称为冷无釉区（AFE）。为探讨无釉区形成的原因，我们观察了大鼠内釉上皮基底膜和远端的超微结构。在老鼠身上。牙釉质覆盖的牙本质上，胶原纤维垂直于基底膜排列。在EFA上，胶原纤维平行于基底层。这些发现表明，EFA和DCE之间的胶原蛋白排列的差异与基膜中的非周期性微纤维的发育状态有关。(2)关于Meckel软骨细胞的成骨细胞转化：Meckel软骨是一种胎儿器官，出生后不久就具有吸收的最终命运。然而，我们发现当Meckel软骨保持在异位部位或细胞培养时，它表达骨型细胞成分和基质钙化。从体内和体外的这些结果可以清楚地看出，细胞微环境如细胞外基质、细胞增殖和血液促进Meckel软骨细胞向成骨细胞的表型转化。(3)用新的器官培养方法培养细胞牙骨质：我们报道了一种新的器官培养方法，通过这种方法可以获得几乎正常的根形成发育。通过这种培养方法，三维器官发生和上皮间质的相互作用可以保持。在这项研究中，我们首次显示了细胞牙骨质在磨牙器官系统在体外形成。