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The studies of the tooth germ differentiation and the transformation of chondrocytes in vitro.

牙胚分化和软骨细胞体外转化的研究。

基本信息

批准号：
16591833
负责人：
NAWA Tokio
金额：
$ 2.24万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591833/
关键词：
tooth germ development Meckel's cartilage calcification lipogenesis differentiation osteoclasts development

项目摘要

(1)The studies on the transformation of Meckel's chondrocytes : Chick serum(CKS) contains factors that stimulate adipocyte induction in Mekel's chondrocytes in vitro. We examined whether sex horomones as shown by the analysis of hormone level in CKS have the capacity to induce lipogenesis in Meckel's chondrocytes in vitro. At pharmacological concentrations, these hormones induced dose-dependent lipogenesis in Merkel's chondrocytes. Further analysis of the fatty acid level in CKS revealed significant contains to fatty acids such as oleic acid and docosatetraenoic acid. We were to establish a mimed fatty acid-containing that had the potential to induced adipogenesis in Meckel's chondrocytes in vitro. In conclusion, it may be suggested that fatty acid elements in CKS can induce Meckel's chondrocytes to differentiate into adipocytes simply and at high frequency.(2)The differentiation of the tooth germ : Hertiwig's epithelial root sheath(HERS) plays an important role in tooth formation. To elucidate the mechanism of root formation in tooth development, we examined the role of insulin-like growth factor(IGF-I) in organ culture. The presence of IGF-I resulted in elongation of HERS and increased cell proliferation in it's outer layer. These effects were negated by the addition of antibodies specific for IGF-I. From these finding, it may be suggested that IGF-I related in the root formation.

（1）Meckel软骨细胞转化的研究：鸡血清（Chick serum，CKS）中含有促进Meckel软骨细胞向脂肪细胞转化的因子。我们研究了性激素是否如CKS中激素水平分析所示具有诱导体外Meckel软骨细胞脂肪生成的能力。在药理学浓度下，这些激素诱导默克尔软骨细胞中的剂量依赖性脂肪生成。进一步分析CKS中的脂肪酸水平，发现CKS中含有大量的脂肪酸，如油酸和二十二碳四烯酸。我们要建立一个模拟的脂肪酸，有可能诱导体外Meckel的软骨细胞脂肪形成。因此，CKS中的脂肪酸成分可以简单、高频率地诱导Meckel软骨细胞向脂肪细胞分化。（2）牙胚的分化：上皮根鞘（Hertiwig's epithelial root sheath，HERS）在牙胚形成中起重要作用。为了阐明牙齿发育中牙根形成的机制，我们研究了胰岛素样生长因子（IGF-I）在器官培养中的作用。IGF-I的存在导致HERS延长，并增加其外层的细胞增殖。这些作用被IGF-I特异性抗体的加入所抵消。这些结果提示IGF-I与根的形成有关。