The studies of the tooth germ differentiation and the mineralization of chondrocytes in vitro

牙胚分化及软骨细胞矿化的体外研究

• 批准号: 13671909
• 负责人: NAWA Tokio
• 金额: $ 1.92万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671909/
• 关键词: Tissue culture; Tooth germ; Mineralization; Cell differentiation; 石灰化; メツケル軟膏; 軟膏培養

1)The studies on the differentiation of Meckel' s cartilage(1) Formation of the shenomandibular ligament by Meckel's cartilage : To clarify the mechanism of formation of this ligament by Meckel's cartilage in mouse, we examined the effects of epidermal growth factor (EGF) on the chondrocytes in terms of the proliferation and differentiation of cells and calcification of the matrix in vivo and in vitro. EGF enhanced DNA synthesis and the proliferation of Meckel's chondrocytes, inhibited the differentiation of cells to chondrocytes and then induced phenotypic changes in fibroblastic cells.(2) Cell death in Meckel's chondrocytes by heat-shock treatment : We examined whether the cell death induced in Meckel's chondrocytes by heat-shock treatment is apoptosis or necrosis, and particular attention was paid to the expression of heat shock protein (HSP) -70 and -27 to analyze the involvement of HSPs in apoptosis.(3)Phenotypic conversion to adipocyes from Meckel's chondrocyte by the treatment o … More f chick serum : When present in the culture medium, chick serum (CKS) modulated the phenotypic change from chondrocytes of Meckel's cartilage to adipocytes in vitro. The adipocytes are induce simply and high frequency by agents in chick serum.2)The phenotypic conversion of chondrocytes to osteoblastic cells : In order to analyze the phenotypic conversion of chondrocytes, mandibular condyles (MMC) of mice and rabbits were cultured under cell and organ culture systems, and then examined by a combination of morphological and biochemical procedures. It results suggest that, morphologically and biochemically, cellular modification in MCC cells under culture conditions occurs at a cellular morphological level and also at marker-gene-expression level.3)Dental root formation in organ culture system : Dental root formation is closely related the elongation of Hertwig's epithelial root sheath(HERS). The study examined the effects of exogenous IGF-I, anti-IGF-I antibody on the early HERS elongation of mouse molar in organ culture system. Less

1)Meckel软骨分化的研究（1）Meckel软骨形成的下颌韧带：为了阐明Meckel软骨形成下颌韧带的机制，我们观察了表皮生长因子（EGF）在体内和体外对软骨细胞增殖、分化和基质钙化的影响。EGF促进Meckel软骨细胞的DNA合成和增殖，抑制细胞向软骨细胞的分化，然后诱导成纤维细胞的表型改变。(2)热休克处理引起的Meckel软骨细胞的细胞死亡：我们检测了热休克处理引起的Meckel软骨细胞的细胞死亡是凋亡还是坏死，并特别注意热休克蛋白（HSP）-70和27的表达，以分析HSP参与凋亡。（3）通过用脂质体处理从Meckel软骨细胞向脂肪细胞的表型转化。 ...更多信息 f鸡血清：当存在于培养液中时，鸡血清（CKS）调节体外Meckel软骨细胞向脂肪细胞的表型变化。2）软骨细胞向成骨细胞的表型转化：将小鼠和家兔的下颌骨髁状突（MMC）置于细胞和器官培养系统中培养，通过形态学和生物化学相结合的方法观察软骨细胞向成骨细胞的表型转化。结果表明，在培养条件下，MCC细胞的形态学和生物化学改变不仅发生在细胞形态学水平，而且发生在标记基因表达水平。3）器官培养体系中牙根的形成：牙根的形成与Hertwig上皮根鞘（HERS）的伸长密切相关。本研究在体外器官培养系统中，观察外源性IGF-Ⅰ、抗IGF-Ⅰ抗体对小鼠磨牙HERS早期延长的影响。少

项目成果

K.Ishizeki, N.Takahashi, T.Nawa: "Phenotypic characteristics of adipocytes generated from Meckel's condrocytes in response to chick serum in vitro"Cell Tissue Res.. 309. 251-260 (2002)

K.Ishizeki、N.Takahashi、T.Nawa：“体外对鸡血清反应中梅克尔软骨细胞生成的脂肪细胞的表型特征”细胞组织研究 309. 251-260 (2002)

K.Ishizeki, N.Takahashi and T.Nawa: "Phenotypic characteristics of adipocytes generated from Meckel's chondrocytes in response to chick serum in vitro"Cell Tissue Res.. 309. 251-260 (2002)

K.Ishizeki、N.Takahashi 和 T.Nawa：“Meckel 软骨细胞产生的脂肪细胞响应体外鸡血清的表型特征”Cell Tissue Res.. 309. 251-260 (2002)

H.Inoue, Y.Hiraki, T.Nawa, K.Ishizeki: "Phenotypic switching of in vitro mandibular condylar cartilage during matrix mineralization"Anatomical Science International. 77. 237-246 (2002)

H.Inoue、Y.Hiraki、T.Nawa、K.Ishizeki：“基质矿化过程中体外下颌骨髁软骨的表型转换”国际解剖科学。

K.Ishizeki, N.Takahashi, T.Nawa: "Phenotypic characteristics of adipocytes generated from Meckel's chondrocytes in response to chick serum in vitro"Cell Tissue Res.. 309. 251-260 (2002)

K.Ishizeki、N.Takahashi、T.Nawa：“体外对鸡血清反应中梅克尔软骨细胞产生的脂肪细胞的表型特征”细胞组织研究 309. 251-260 (2002)

K.Ishizeki, N.Takahashi and T.Nawa: "Formation of the sphenomandibular ligament by Meckel's cartilage in the mouse : possible involvement of epidermal growth factor as revealed by studies in vivo and in vitro"Cell Tissue Res. 304. 67-80 (2001)

K.Ishizeki、N.Takahashi 和 T.Nawa：“小鼠梅克尔软骨形成蝶下颌韧带：体内和体外研究揭示表皮生长因子可能参与”Cell Tissue Res。