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Molecullar Biology on the Calcification of Cartilages and Tooth Germs in Vito.

维托软骨和牙胚钙化的分子生物学。

基本信息

批准号：
09671862
负责人：
NAWA Tokio
金额：
$ 2.05万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671862/
关键词：
Tooth germ Cartilage Meckel's cartilage Cacification Tissue cilture Tooth germ cartilage Cacification cementum ename-free area tissue culture calcification

项目摘要

1) The study on the fate of Meckel's cartilage.The Meckel's cartilage chondrocytes can express the osteocytic phenotype in vitro during synthesis of bone-type marker proteins such as osteocalcin, osteopontin and type I collagen. The midportion of Meckel's cartilage was subdivided into two regions, the anterior and posterior regions.The our results suggest that cells in the midportion of Meckels cartilage have two different possible fates. The anterior is dissolved by TRAP-positive cells, while chondrocytes in the posterior midportion are transformed directly into fibroblastic cells by phenotypic conversion.2) The study on the development of enanamel-free area of rodent molarsBoth the expression of amelogenin mRNA and secretion of amelogenin were investigated in rat molars by in situ hybridization and immunohistohemistry.Our results suggest that enamel-free area cells differentiated into amelogenin secreting cells, I.e. ameloblasts, but that the seretion lasts for only a short period at the early stage of tooth development. A role for amelogenin in inner enamel epithelium at the early stage of tooth development may emerge in the future.3)The study on the formation of cementum in vitro.A new organ culture system for in vitro study of cementogenesis in mouse molars teeth was developed. This culture method maintained the three-dimensional architecture of tooth germ. By the 43rd culture day, acellular cementum-like layer, clearly different from the periodontal ligament proper, was formed on the acellular cementum.

1)Meckel‘s软骨命运的研究：Meckel’s软骨细胞在体外合成骨钙素、骨桥蛋白、I型胶原等骨型标志蛋白的过程中，能表达骨细胞表型。Meckel‘s软骨中部分为前部和后部两个区域，提示Meckels软骨中部细胞可能有两种不同的命运。前牙由TRAP阳性细胞溶解，后中段软骨细胞通过表型转化直接转化为成纤维细胞。2)鼠磨牙牙釉质脱落区发育的研究用原位杂交和免疫组织化学方法研究了大鼠磨牙牙釉质脱落区的表达和釉原蛋白的分泌。结果表明，在牙齿发育早期，脱釉区细胞分化为釉原蛋白分泌细胞，即成釉细胞。3)体外牙骨质形成的研究。建立了一种新的小鼠磨牙牙骨质发生的体外器官培养体系。这种培养方法保持了牙胚的三维结构。培养第43天，脱细胞牙骨质表面形成明显不同于牙周韧带的脱细胞牙骨质样层。