Relationship between the Induction of Histamine-Forming Enzyme and Pain in Muscle Fatique and in Arthrosis Temporomandibularis

组胺形成酶的诱导与肌肉疲劳和颞下颌关节疼痛的关系

• 批准号: 06454530
• 负责人: ENDO Yasuo
• 金额: $ 3.52万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06454530/
• 关键词: histamine; muscle fatigue; muscle pain; interleukin-1; histidine decarboxylase; arthrosis temporomandibularis; anti-histamine; 筋肉筋; 骨格筋; ヒスチジンデカルボキシラーゼ

Induction of histidine decarboxylase (HDC), the histamine-forming enzyme, in the skeletal muscle was examined in mice under various conditions, and following results were obtained :1. Electrical stimulation of quadriceps femoris or masseter muscle increased activity in the stimulated muscles.2. Prolonged walking also elevated HDC activity in quadriceps femoris muscles.3. Mast cells, histamine-storing cells, were shown to be not involved in the induction of HDC activity in the sleketal muscles.4. An anti-histaminergic drug, chlorphenylamine, were more effective than an Anti-inflammatory analgesic, flurbiprofen in improving various symptoms in the patients with arthrosis temporomandibularis.5. Injection of as little as 1 mug/kg of interleukin-1 (IL-1) elevated HDC activity in quadriceps femoris and masseter muscles. The time course of the HDC elevation induced by IL-1 was more rapid than that induced by electrical stimulation and prolonged walking.6. IL-1beta was histochemically detected in quadriceps femoris and masseter muscles. In addition to capillary vessels, a larger amount of IL-1beta was detected in the mitochondria of these muscle cells.7. The possibility that lacticacid, which is called"fatique substance", may induce HDC activity in the skeletal muscle was suggested to be unlikely.8. The extent of HDC induction in skeletal muscles was shown to depend on the sex, age, training of physical exercise and on the strain of the mouse.These results support our hypothesis that the histamine formed through the IL-1-mediated induction of HDC is involved in fatique and pain in skeletal muscle and indicate that anti-histaminergic drugs are useful for improving symptoms of arthrosis tempor omandibularis.

研究了不同条件下小鼠骨骼肌中组胺形成酶组氨酸脱羧酶（HDC）的诱导作用，得到以下结果：电刺激股四头肌或咬肌可增加受刺激肌肉的活动性。长时间步行也会增加股四头肌HDC的活动。肥大细胞，组胺储存细胞，显示不参与诱导HDC活动在腱状肌。抗组胺药氯苯胺在改善颞下颌关节病患者的各种症状方面比抗炎镇痛药氟比洛芬更有效。仅注射1杯/公斤的白细胞介素-1 （IL-1）就能提高股四头肌和咬肌中HDC的活性。IL-1诱导的HDC升高时间过程比电刺激和长时间步行诱导的HDC升高时间过程要快。在股四头肌和咬肌组织化学检测il -1 β。除毛细血管外，在这些肌细胞的线粒体中检测到大量的il -1 β。被称为“疲劳物质”的乳酸诱导骨骼肌HDC活动的可能性不大。骨骼肌中HDC诱导的程度取决于小鼠的性别、年龄、体育锻炼的训练和品系。这些结果支持了我们的假设，即通过il -1介导的HDC诱导形成的组胺参与了骨骼肌的疲劳和疼痛，并表明抗组胺能药物可用于改善关节关节炎的症状。

项目成果

Takanobu Ando: "Stimulation of the synthesis of histamine and putrescine in mice by a peptidoglycan of Gram-positive bacteria" Microbiology and Immunology. 38. 209-215 (1994)

Takanobu Ando：“革兰氏阳性菌的肽聚糖刺激小鼠体内组胺和腐胺的合成”微生物学和免疫学。

Takanobu Ando, Yasuo Endo, Masatake Abe and Katsuo Kumagai: "Stimulation of the synthesis of histamine and putrescine in mice by a peptidoglycan of Gram-positive bacteria" Microbiology and Immunology. 38. 209-215

Takanobu Ando、Yasuo Endo、Masatake Abe 和 Katsuo Kumagai：“革兰氏阳性菌肽聚糖刺激小鼠体内组胺和腐胺的合成”微生物学和免疫学。

Yasuo Endo, Masanori Nakamura, Yasutake Nitta and Katsuo Kumagai: "Effects of macrophage depletion on the induction of histidine decarboxylase, the enzyme forming histamine, by lipopolysaccharide, interleukin-1 and tumor necrosis factor" British Journal o

Yasuo Endo、Masanori Nakamura、Yasutake Nitta 和 Katsuo Kumagai：“巨噬细胞耗竭对脂多糖、白细胞介素 1 和肿瘤坏死因子诱导组氨酸脱羧酶（形成组胺的酶）的影响”英国杂志 o

遠藤康男: "ヒスタミン合成酵素のサイトカインによる調節" 蛋白質核酸酵素. 39. 1440-1444 (1994)

Yasuo Endo：“细胞因子诱导的组胺合酶调节”蛋白质核酸酶 39. 1440-1444 (1994)

Yasuo Endo: "Effect of mucrophagy depletion on the induction of histidine decarboxylase,the eneyme forminghistamine,by LPS,IL-1 and TNF." British Jounal of Pharmacology. 114. 187-193 (1995)

Yasuo Endo：“吞噬细胞吞噬对 LPS、IL-1 和 TNF 诱导组氨酸脱羧酶（形成组胺的敌人）的影响。”