Relationship between the Induction of Histamine-Forming Enzyme and Pain in Muscle Fatique and in Arthrosis Temporomandibularis

本实验研究了不同条件对小鼠骨骼肌中组胺形成酶--组氨酸脱羧酶（HDC）的诱导作用。电刺激股四头肌或咬肌增加了受刺激肌肉的活动。长时间的步行也会增加股四头肌中HDC的活性。肥大细胞，组胺储存细胞，被证明是不参与诱导的HDC活性在sleketal肌肉.抗组胺药氯苯那敏在改善颞下颌关节炎患者的各种症状方面优于抗炎镇痛药氟比洛芬.注射低至1 μ g/kg的白细胞介素-1（IL-1）可提高股四头肌和咬肌中HDC的活性。IL-1诱导的HDC升高的时间进程比电刺激和长时间步行诱导的HDC升高的时间进程快.用组织化学方法检测股四头肌和咬肌中IL-1 β的表达。除了毛细血管外，在这些肌细胞的线粒体中检测到大量的IL-1 β。7.被称为“疲劳物质”的乳酸可能诱导骨骼肌中HDC活性的可能性被认为是不可能的。骨骼肌HDC的诱导程度与小鼠的性别、年龄、运动训练和品系有关，这些结果支持了我们的假设，即通过IL-1介导的HDC诱导形成的组胺参与了骨骼肌的疲劳和疼痛，并表明抗组胺药物对改善颞下颌关节炎的症状是有用的。

Induction of histidine decarboxylase (HDC), the histamine-forming enzyme, in the skeletal muscle was examined in mice under various conditions, and following results were obtained :1. Electrical stimulation of quadriceps femoris or masseter muscle increased activity in the stimulated muscles.2. Prolonged walking also elevated HDC activity in quadriceps femoris muscles.3. Mast cells, histamine-storing cells, were shown to be not involved in the induction of HDC activity in the sleketal muscles.4. An anti-histaminergic drug, chlorphenylamine, were more effective than an Anti-inflammatory analgesic, flurbiprofen in improving various symptoms in the patients with arthrosis temporomandibularis.5. Injection of as little as 1 mug/kg of interleukin-1 (IL-1) elevated HDC activity in quadriceps femoris and masseter muscles. The time course of the HDC elevation induced by IL-1 was more rapid than that induced by electrical stimulation and prolonged walking.6. IL-1beta was histochemically detected in quadriceps femoris and masseter muscles. In addition to capillary vessels, a larger amount of IL-1beta was detected in the mitochondria of these muscle cells.7. The possibility that lacticacid, which is called"fatique substance", may induce HDC activity in the skeletal muscle was suggested to be unlikely.8. The extent of HDC induction in skeletal muscles was shown to depend on the sex, age, training of physical exercise and on the strain of the mouse.These results support our hypothesis that the histamine formed through the IL-1-mediated induction of HDC is involved in fatique and pain in skeletal muscle and indicate that anti-histaminergic drugs are useful for improving symptoms of arthrosis tempor omandibularis.