权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Genetic study on the mutants for protein body in rice endosperm to improve the grain quality.

水稻胚乳蛋白体突变体改良稻米品质的遗传研究

基本信息

批准号：
59480027
负责人：
IWATA Nobuo
金额：
$ 4.03万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480027/
关键词：
Breeding of grain quality Rice storage protein Protein body Fertilized egg cell treatment with MNU SDS-PAGE ELISA Two dimentional electrophresis 遺伝子分析

项目摘要

The rice strage proteins deposite in the two types of protein bodies, PB- and PB <II> , which are characterized from each other by differences in the morphology and the polypeptide composition. This study deals with the screening of mutants for protein bodies in rice endosperm, and characterization and genetic analysis of the mutants to obtain genetic resources for qualitative improvement of rice storage protein. The results are given as follows:1) From the results of the analysis of the isolated PB- , its polypeptide composition and digestibility were cleared.2) Seventeen mutant lines for protein body were selected from about 3000 mutant lines induced by the treatment of the fertilized egg cell with MNU and fourty six varieties were obtained from about 2000 rice varieties by SDS-PAGE.3) They have been classified into four types on the basis of variation of the relative content of the polypeptide.4) In determination of extracted protein in the starchy endosperm of the mutants, it was revealed that the content of prolamin and glutelin but not globulin were brought on a change.5) By the SDS-PAGE analysis of protein bodies isolated from the endosperm of four types of mutants, it was clarified that three were concerned with PB- .6) Gene analysis of three types of mutants showed that each of them was controlled by different single recessive genes. The trisomic analysis indicated that two genes for mutated polypeptide, rsp-1 and rsp-2, located on chromosome 9 and 10 respectively.7) The mutants for quantitative changes in each polypeptide of protein body were obtained by immune react.

水稻籽粒蛋白质以PB-和PB-两种蛋白体形式存在<II>，这两种蛋白体在形态和多肽组成上存在差异。本研究通过对水稻胚乳蛋白体突变体的筛选、鉴定和遗传分析，为水稻贮藏蛋白质的品质改良提供遗传资源。结果如下：1）从分离的PB-的分析结果来看，2）用MNU处理水稻受精卵细胞，从3000多个突变株系中筛选出17个蛋白体突变株系，用SDS-PAGE从2000多个水稻品种中筛选出46个品种; 3）根据蛋白体相对含量的变化，将它们分为4类;对4种突变体胚乳蛋白质的SDS-PAGE分析表明，其中3种与PB-有关。6）对3种突变体的基因分析表明，这3种突变体均受不同的单隐性基因控制。三体分析表明，rsp-1和rsp-2基因分别位于第9和第10染色体上。7）通过免疫反应获得蛋白体各多肽数量变化的突变体。