An approach to construction of chromosome map of rice.

水稻染色体图谱构建方法.

基本信息

  • 批准号:
    62480033
  • 负责人:
  • 金额:
    $ 4.16万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1987
  • 资助国家:
    日本
  • 起止时间:
    1987 至 1989
  • 项目状态:
    已结题

项目摘要

Many chromosomal deficiencies were successfully induced and selected in F^1 obtained by pollinating r-ray irradiated pollens to genetic marker stocks. Normal varieties were pollinators and the plants themselves were irradiated r-ray (5KR) one day before anthesis. We obtained 19 plants of 2n = 23 + 1 fragment, 29 monosomic plants, and many plants which showed pseudodominance but no deficiency detected in microscopic level. All of the F^1 plants derived from crossing the 2n = 23 + 1 fragment and monosomic plants with disomics were all disomics, indicating that the chromosome deficiency was not transmitted to the next generation. We also obtained three plants of 2n = 24 + 1 fragment through the selfing r-ray irradiated trisomic plants or through the pollinating r-ray irradiated pollens of normal plants to trisomics with genetic markers. There was much prospect to get monotelotrisomics. Irradiation of r-ay one day before anthesis was very effective for inducing and selecting reciprocal tra … More nslocation and more than one hundred reciprocal translocation homozygotes were bred. Eight types of tertiary trisomics were also derived from crossing reciprocal translocation heterozygote primary trisomics with normal plants. The several kinds of chromosome alternations developed in this study would much contribute further chromosome mapping and gene mapping.We successfully applied fluorescent staining technique with Chromomycin A to identify nucleolus chromosomes. Modified methods of pachytene chromosome preparation made it possible to appear fully spreading pachytene chromosomes even in fixed materials.It was possible to Infer the chromosomal location of breakpoint of some reciprocal translocations through karyotype and pachytine chromosome analysis. Based on the linkage informations between the breakpoints and genetic markers, we also could estimate chromosomal location of the genetic markers or chromosomal orientation such as arm location of linkage maps regarding chromosomes 1, 3 and 11. Less
用辐射处理的花粉为遗传标记库授粉获得的F^1代,成功地诱导和选择了许多染色体缺失。正常品种是传粉者,植株本身在开花前一天接受5KR的辐射。获得了19株2n=23+1片段,29株单体植株,以及许多在显微水平上表现为假显性但未检测到缺失的植株。与2n=23+1片段杂交产生的F^1植株和二体植株均为二体,说明染色体缺失不会遗传给下一代。我们还通过自交r射线照射的三体植株或通过授粉的正常植株的r射线照射的花粉对具有遗传标记的三体植株进行授粉,获得了3株2n=24+1片段。获得单体三体的前景很大。开花前一天照射r-Ay对反式…的诱导和选择非常有效共培育出100多个ns位和100多个相互易位的纯合子。还将易位杂合子一级三体与正常植物杂交,获得了8种类型的三体。本研究中开发的几种染色体改变将对进一步的染色体定位和基因定位有很大的贡献。我们成功地应用荧光染色技术与显色素A一起识别核仁染色体。改进的粗线期染色体制备方法使粗线期染色体即使在固定材料中也能完全展开,并可通过核型分析和粗线期染色体分析推测某些相互易位的染色体断裂点。基于断裂点和遗传标记之间的连锁信息,我们还可以估计遗传标记的染色体位置或染色体方向,如1、3和11染色体的连锁图谱的臂位置。

项目成果

期刊论文数量(26)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Iwata,N.: "Linkage map for Nishimura's chromosome 8." Rice Genetics Newsletter(in press). 6. (1990)
Iwata,N.:“西村 8 号染色体的连锁图。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Wang,Z.X.: Rice Genetics Newsletter. 5. (1988)
王ZX:水稻遗传学通讯。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Wang,Zi-Xuan: "A trial to induce chromosome deficiencies and monosomics in riceby using irradiated pollen." Rice Genetics Newsletter. 5. 64-65 (1988)
王子轩:“利用辐照花粉诱导水稻染色体缺陷和单体的试验。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Wang, Z. X., N. Iwata, Y. Sukekiyo, A. Yoshimura and T. Omura.: "A trial to induce chromosome deficiencies and monosomics in rice by using irradiated pollen." Rice Genetics Newsletter 5:64-65, 1988.
Wang, Z. X., N. Iwata, Y. Sukekiyo, A. Yoshimura 和 T. Omura.:“利用辐照花粉诱导水稻染色体缺陷和单体的试验。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Wang,Z.X.: "A trial to induce chromosome deficiencies and monosomics in rice by using irradiated pollen." Rice Genetics Newsletter. 5. 64-65 (1989)
Wang, Z.X.:“利用辐照花粉诱导水稻染色体缺陷和单体的试验。”
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  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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IWATA Nobuo其他文献

IWATA Nobuo的其他文献

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{{ truncateString('IWATA Nobuo', 18)}}的其他基金

NEW APPROACH FOR RICE GENE MAPPING
水稻基因图谱的新方法
  • 批准号:
    03404005
  • 财政年份:
    1991
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (A)
Qualitative and Quantitative Improvement of Seed Storage Proteins in Rice
水稻种子贮藏蛋白的定性和定量改良
  • 批准号:
    63860003
  • 财政年份:
    1988
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B).
Genetic study on the mutants for protein body in rice endosperm to improve the grain quality.
水稻胚乳蛋白体突变体改良稻米品质的遗传研究
  • 批准号:
    59480027
  • 财政年份:
    1984
  • 资助金额:
    $ 4.16万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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