NEW APPROACH FOR RICE GENE MAPPING

水稻基因图谱的新方法

• 批准号: 03404005
• 负责人: IWATA Nobuo
• 金额: $ 13.95万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03404005/
• 关键词: Rice; Conventional linkage map; RFLP linkage map; Integrated linkage map; Chromosome map; Cytogenetical variants; RAPD markers; RI lines; 組換価; RFLP連鎖地図; トリソミック分析; 近似同質遺伝子系統; 地図情報

To furnish significant information to rice molecular genetrics and breeding, we have integrated the conventional linkage map onto the RFLP linkage map. Twenty-four F_2 populations segregating genetic markers and bacterial blight resistance genes were investigated. Fourty-eight genes for the morphological and physiological traits were successfully mapped on RFLP linkage maps as follows : Chromosome 1, fs-1, d-18, d-2, rl-2, spl-6 and eg, Chromosome 2, tri, bl-1, l-chl (t) and spl-2 ; Chromosome 3, chl-1, fc-1, st (t), dl and spl-3 ; Chromosome 4, d-11, Ph, lg, Xa-1 and Xa-2 ; Chromosome 5, gl-1, xa-5, d-1, st (t), spl-7 and nl-1 ; Chromosome 6, dp-1, spl-4 and Cl ; Chromosome 7, g-1, d-6, spl-5 and v-11 ; Chromosome 8, v-8 and sug, Chromosome 9, I-Bf, dp-2, drp-2 and Dn-1 ; Chromosome 10, spl-10 and pgl ; Chromosome 11, Xa-3, Xa-4, Xa-10, z-2 and v-9 ; and Chromosome 12, spl-1 and rl-1 The orientation of the classical linkage map and the RFLP linkage map was completely understood and the classical linkage map was a little revised from this study. Some RFLP markers were found to be closely linked to the genes for the morphological and physiological traits, indicating that they could be useful for map-assisted selection in rice breeding program and for map-based cloning of the genes as starting points.The physical association of the linkage map with chromosomes using cytogenetical variants such as reciprocal translocation, tertiary trisomics and acrotrisomics were also examined. The orientation of linkage groups, the arm location of marker genes and inferred regions of centromeres were decided on chromosomes 1,3,4,5,7,9 and 12.In addition, recombinant inbred (RI) lines were developed as a permanent mapping population for RFLP/RAPD markers. Using the RI lines, landmark linkage map containing 119 RFLP loci and 26RAPD loci were constructed.

为了给水稻分子遗传学和育种提供有意义的信息，我们将传统的连锁图整合到RFLP连锁图上。对24个F_2群体的遗传标记和白叶枯病抗性基因进行了分离分析。在RFLP连锁图谱上，共定位了48个形态和生理性状基因，分别为：第1染色体fs-1、d-18、d-2、rl-2、spl-6和eg，第2染色体tri、bl-1、l-chl（t）和spl-2，第3染色体chl-1、fc-1、st（t）、dl和spl-3，第4、5、6、7、8、9、10、11、12、13、14、15、16、17、18、19、染色体4，d-11，Ph，lg，Xa-1和Xa-2 ;染色体5，gl-1，xa-5，d-1，st（t），spl-7和nl-1 ;染色体6，dp-1，spl-4和Cl ;染色体7，g-1，d-6，spl-5和v-11 ;染色体8，v-8和sug，染色体9，I-Bf，dp-2，drp-2和Dn-1 ;染色体10，spl-10和pgl ;染色体11，Xa-3，Xa-4，Xa-10，z-2和v-9 ; 12号染色体、spl-1和rl-1。本研究对经典连锁图和RFLP连锁图的定位有了较全面的了解，对经典连锁图作了一些修正。结果表明，部分RFLP标记与形态和生理性状基因紧密连锁，可用于水稻育种的图位辅助选择和图位克隆，并利用相互易位、三级三体和顶体三体等细胞遗传变异研究了连锁图与染色体的物理关联。在第1、3、4、5、7、9、12号染色体上确定了连锁群的定位、标记基因的臂定位和着丝粒的推断区域。利用RI系构建了包含119个RFLP位点和26个RAPD位点的连锁图谱。

项目成果

Wang,Z.X.and N.Iwata.: "Production of n+1 plants and tetrasomics by means of anther culture of trisomic plants in rice(Oryza sativa L.)." Theoretical and Applied Genetics.83. 12-16 (1991)

Wang, Z.X. 和 N.Iwata.：“通过水稻三体植物的花药培养生产 n 1 植物和四体体（Oryza sativa L.）”。

Wang,Z.X.,N.Iwata,Y.Sukekiyo and A.Yoshimura.: "Induction of chromosome aberrants in rice(Oryza sativa L.)by using irradiated pollen." J.Fac.Agr.,Kyushu Univ.36. 99-108 (1991)

Wang，Z.X.，N.Iwata，Y.Sukekiyo 和 A.Yoshimura.：“利用辐射花粉诱导水稻（Oryza sativa L.）染色体异常。”

Ideta,O.,A.Yoshimura,T.Matsumoto and H.Tsunematsu and N.Iwata.: "Integration of conventional and RFLP maps in rice.I.Chromosomes 1,2,3and 4." Rice Genetics Newsletter. 9. 128-129 (1992)

Ideta,O.,A.Yoshimura,T.Matsumoto 和 H.Tsunematsu 和 N.Iwata.：“水稻中常规图谱和 RFLP 图谱的整合。I.染色体 1、2、3 和 4。”

Nonomura,K.I.,A.Yoshimura,N.Kishimoto and N.Iwata: "Gene mapping on rice pachytene chromosome by using reciprocal translocation and tertiary trisomics;orientation of linkage groups corresponding to the chromosomes 1,3,4,5,7,9and12." Proc.7th Intern.Congr.

Nonomura、K.I.、A.Yoshimura、N.Kishimoto 和 N.Iwata：“利用相互易位和三级三体对水稻粗线期染色体进行基因定位；对应于染色体 1、3、4、5、7、9 和 12 的连锁群的方向

Yoshimura,S.,A.Yoshimura and N.Iwata: "Simple and rapid PCR method by using crude extract from rice seedings." Japan.J.Breed.42. (1992)

Yoshimura,S.、A.Yoshimura 和 N.Iwata：“利用稻种粗提物进行简单快速的 PCR 方法。”