NEW APPROACH FOR RICE GENE MAPPING

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To furnish significant information to rice molecular genetrics and breeding, we have integrated the conventional linkage map onto the RFLP linkage map. Twenty-four F_2 populations segregating genetic markers and bacterial blight resistance genes were investigated. Fourty-eight genes for the morphological and physiological traits were successfully mapped on RFLP linkage maps as follows : Chromosome 1, fs-1, d-18, d-2, rl-2, spl-6 and eg, Chromosome 2, tri, bl-1, l-chl (t) and spl-2 ; Chromosome 3, chl-1, fc-1, st (t), dl and spl-3 ; Chromosome 4, d-11, Ph, lg, Xa-1 and Xa-2 ; Chromosome 5, gl-1, xa-5, d-1, st (t), spl-7 and nl-1 ; Chromosome 6, dp-1, spl-4 and Cl ; Chromosome 7, g-1, d-6, spl-5 and v-11 ; Chromosome 8, v-8 and sug, Chromosome 9, I-Bf, dp-2, drp-2 and Dn-1 ; Chromosome 10, spl-10 and pgl ; Chromosome 11, Xa-3, Xa-4, Xa-10, z-2 and v-9 ; and Chromosome 12, spl-1 and rl-1 The orientation of the classical linkage map and the RFLP linkage map was completely understood and the classical linkage map was a little revised from this study. Some RFLP markers were found to be closely linked to the genes for the morphological and physiological traits, indicating that they could be useful for map-assisted selection in rice breeding program and for map-based cloning of the genes as starting points.The physical association of the linkage map with chromosomes using cytogenetical variants such as reciprocal translocation, tertiary trisomics and acrotrisomics were also examined. The orientation of linkage groups, the arm location of marker genes and inferred regions of centromeres were decided on chromosomes 1,3,4,5,7,9 and 12.In addition, recombinant inbred (RI) lines were developed as a permanent mapping population for RFLP/RAPD markers. Using the RI lines, landmark linkage map containing 119 RFLP loci and 26RAPD loci were constructed.