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Studies on early pregnancy diagnosis and fetal physiology in cattle by real-time ultrasonography

实时超声对牛早期妊娠诊断及胎儿生理的研究

基本信息

批准号：
59480085
负责人：
TOO Kimehiko
金额：
$ 3.71万
依托单位：
HOKKAIDO UNIVERSIY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1984
资助国家：
日本
起止时间：
1984 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59480085/
关键词：
Ultrasonography Early pregnancy diagnosis in cattle Fetal physiology Fetal heart rate Embryo transfer ウシ双胎妊娠診断

项目摘要

By real-time ultrasonography,observation of images of uteruses during the estrous cycle,diagnosis of early pregnancy,observation of fetuses and investigate to fetal physiology from fetal heart beat were performed in total 93 dairy cattle. The results obtained are summarized as follows;1. Early pregnancy diagnosis of heifers was performed between 17 and 24 days after artificial insemination(AI).Clear fetal sac (FS) showing echofree area images indicating pregnancy were obtained between 22 and 24 days after AI in 95% of pregnant cattle. But it was difficult to distinguish between FS images and uterine mucus fluid images. Accuracy of pregnancy diagnosis was 25% between 17 and 21 days,86.4% between 22 and 24 days and 45.2% in total.2. The fetuses were first visible on 29 days after AI, and thier images were taken by rectal sanning up to about 150 days of gestation.3. Fetal crown-rump length(CRL) measurements were made from 29 to 53 days of gestation. A highly significant positive correlation was observed between the fetal age and CRL(gamma=0.926). There was no difference of the correlation among fetuses resulted from AI,embryo transfer and bisected embryo transfer.4. Fetal internal structure image could be seen after about 60 days of gestation. Acoustic shadows caused by fetal skeltons were not observed at the beginning. Interventricular septum,right and left ventricular chamber of fetal heart could be seen after 80 or 90 days of gestation.5. Correlation between the fetal age and the fetal heart rate was positive slightly up to about 60 days and the after it was negative highly. Variation of fetal heart rate in short term was observed and the mean variation was 20/min.6. Twins resulted from embryo transfer or bisected embryo transfer could be diagnosed on 34,34,36,77 and 83 days of gestation which confirmed that USG could be applied to determine fetal numbers in early gestational period.

应用实时超声技术，对93头奶牛进行了发情周期子宫图像观察、早期妊娠诊断、胎儿形态观察和胎心生理研究。主要研究结果如下：1.对人工授精后17 ~ 24 d的小母牛进行早期妊娠诊断，95%的妊娠牛在人工授精后22 ~ 24 d获得清晰的胎囊（FS），显示妊娠的无回声区图像。但FS图像与子宫粘液图像难以区分。17 ~ 21天妊娠诊断准确率为25%，22 ~ 24天妊娠诊断准确率为86.4%，总妊娠诊断准确率为45.2%. AI后29天首次可见胎儿，并在妊娠约150天内通过直肠扫描拍摄胎儿图像。在妊娠第29 ~ 53天测量胎儿顶臀长（CRL）。胎龄与CRL呈显著正相关（γ =0.926）。人工授精、胚胎移植和分割胚胎移植所产胎儿之间的相关性无差异.妊娠60天左右可见到胎儿内部结构图像。在开始时未观察到由胎儿声束引起的声影。孕80 ~ 90天可见室间隔、左右心室腔.胎龄与胎心呈轻度正相关，60天后呈高度负相关。观察胎心率短期内变化，平均变化20次/min.在妊娠34、34、36、77、83 d时，超声心动图可用于诊断胚胎移植或二分胚胎移植的双胞胎，证实超声心动图可用于早期妊娠胎儿数目的测定。