Effects of Smoking and Passive Smoking on Aryl Hydrocarbon Hydroxylase Inducibility

为了定量评价吸烟和被动吸烟对人体的生物学或生化影响，我们测定了111例男性吸烟者(包括36例非吸烟者和128例被动吸烟者)淋巴细胞芳烃羟基酶活性。基础和3-甲基胆蒽(MC)诱导的AHH活性和AHH诱导率(Basal/MC诱导)差异很大，且变化范围很大。我们统计了吸烟和被动吸烟对AHH的影响，并考虑了其他环境因素，如饮酒、喝咖啡、习惯使用药物等对AHH的影响。在所考察的环境因素中，咖啡摄入量与酶活性呈显著正相关，而吸烟和被动吸烟则不显著。然而，我们观察到吸烟量与咖啡摄入量成正比(r=0.45p&lt；0.0001)。因此，无论从生物学角度还是从实际角度来看，咖啡摄入量和吸烟似乎都能增强AHH活性，且咖啡摄入量对AHH活性的影响大于吸烟。此外，吸烟和二手烟对健康影响的初步研究结果显示，吸烟和二手烟对健康状况(如主观症状、现有疾病、病史、收缩压和舒张压)没有显著影响。饮酒与血压呈显著正相关。根据研究结果，吸烟和被动吸烟对AHH活性的影响被认为比我们预期的要小得多，而且那些对健康状况的不良影响似乎比我们所考虑的要小得多。此外，咖啡的摄入和饮用似乎比我们想象的要引起更多的生物效应。

To evaluate the biological or biochemical effect of smoking and passive smoking to man quantitatively, we determined aryl hydrocarbon hydroxylase(AHH) activity in lymphocytes of 111 male smokers, including 36 nonsmokers, and 128 male passive smokers. Basal and 3-methyl-cholanthrene(MC) -induced AHH activity and AHH induction ratio(Basal/MC-Induced) varied so much individually, and were of very wide range. We examined statistically the effect of smoking and passive smoking on AHH, taking the effect of other environmental factors such as drinking, coffee intake, habitual use of medicines and so forth on AHH into account. Among the environmental factors examined, coffee intake showed the significant, positive correlation to the enzyme activity, but smoking and passive smoking did not. We, however, observed that the amount of smoking increased in positive proportion to that of coffee intake(r=0.45, p<0.0001). Therefore from a biological or practical point of view, both coffee intake and smoking seemed to enhance the AHH activity and the effect of coffee intake on AHH was considered to be more than that of smoking. In addition, results of the preliminary study in health consequences of smoking and passive smoking showed that smoking and passive smoking exhibited no significant effect on the condition of health such as subjective symptoms, present sicknesses, histories of sickness, systolic blood pressure and diastolic blood pressure. Drinking showed the significant, positive correlation to the blood pressure. Based on the results of the study,effect of smoking and passive smoking on AHH activity was considered to be much less than we expected, and bad effects of those for the condition of health studied seem to be much less than we have considered. Furthermore, coffee intake and drinking seem to induce much more biological effects than we have thought.