Amphiphilic precision macromolecules to study effects of membrane dynamics (A11#)

在这个提议中，我们的目标是开发一个平台的合成两亲物作为模拟物和生物膜的调节剂。通过固相组装定制的构建块，可以获得结构上限定的合成两亲物，所谓的两亲性精密大分子（AM），其允许分析大范围的结构参数（例如亲水性头部或疏水性尾部基团的几何形状和组成）对膜动力学的影响。与CRC内的合作伙伴一起，AM将被探索以改变膜特性，例如通过创建拥挤的膜表面并研究对膜蛋白的影响，无论是在体外还是在植物中。

Within this proposal, we aim at developing a platform of synthetic amphiphiles as mimetics and modula-tors of biomembranes. Through solid phase assembly of tailor-made building blocks, structurally defined synthetic amphiphiles, so called amphiphilic precision macromolecules (AMs) can be obtained that allow analyzing the effect of a large range of structural parameters such as the geometry and composition of the hydrophilic head or hydrophobic tail group on membrane dynamics. Together with the collaboration partners within the CRC, AMs will be explored to vary membrane properties e.g. by creating crowded membrane surfaces and study the effects on membrane proteins, both in vitro and in planta.