Autoantibody to Hemopoietic Prcgenitors

首先，采用补体依赖性细胞毒性（CDC）试验和克隆培养方法检测患者存在造血祖细胞自身抗体的疾病。在纯红细胞发育不全（PRCA）。CFU-E抗体为5/8，BFU-E抗体为2/8。在特发性血小板减少性紫癜（ITP）中，在所有患者的血清中检测到巨核细胞(M)祖细胞自身抗体。虽然脾切除术患者的脾细胞与抗8-氮杂鸟嘌呤的HMy2杂交，但我们无法获得产生抗m祖细胞抗体的杂交瘤。其次，为了获得具有多能造血祖细胞特征的小鼠单克隆抗体（MoAb） K562细胞，将K562细胞免疫小鼠脾细胞进行SP2/0细胞杂交，获得10个分泌MoAb的杂交瘤。一种新的抗k562细胞单克隆抗体，称为RTF8X，细胞毒性IgM，可识别来自红细胞白血病和真性红细胞增多症患者的红母细胞上的外科抗原。正常外周血和骨髓造血祖细胞（CFU-E和BFU-E）未检出RTF8X抗原。细胞分选分析显示，来自患者和正常志愿者的RTF8X阳性骨髓细胞含有60%以上的红母细胞。（酶处理提示RTF8X抗原为唾液糖脂。）RTF8X可用于疾病的诊断和病程的观察。

Firstly, in order to dctect the disorders in which patients have autoantibody to hemopoietic progenitors, complement dependent cytotoxity (CDC) test and clonogenic culture were used. In pure red cell aplasia (PRCA). autoantibody to CFU-E(5/8) and to BFU-E(2/8) wers demonstrable.In idiopathic thrombocytopenic purpura (ITP), autoantibody to megakaryocyte(M) progenitors were detected in sera from all patients tested. Although spleen cells from the patient who weceived splenectomy were hybridized with HMy2 that are resistant to 8-azaguanine, we could not obtained hybridoma htat produced antibody to M-progenitors.Secondly, in order to obtain murine monoclonal antibody (MoAb) K562 cells that have some characters of multipotent hemopoietic progenitors, spleen cells from mice immunized by K562 cells were hybridized SP2/0 cells and 10 hybridoma secreting MoAb were obtained. A new anti-K562 cell monoclonal antibody, termed RTF8X, cytotoxic IgM, recognibed a surgace antigen on erythroblast from patients with erythroleukemia and polycythemia vera. RTF8X antigen was not detected normal peripheral blood and marrow hemopoietic progenitors (CFU-E and BFU-E). Cell-sorting analysis revealed that RTF8X positive marrow cells from the patients and normal volunteers contained more than 60% of erythroblasts. (Enzyme treatment, suggested that RTF8X antigen was sialo-glycolipid.) RTF8X should be useful to diagnose the disorders and to observe the course of the disorders.