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Allogeneic bone grafts into maxillary alveolar cleft in rats -Bone inductive activity of demineralized bone matrices stimulated by electromagnetic fields-

大鼠上颌牙槽裂同种异体骨移植-电磁场刺激脱矿骨基质的骨诱导活性-

基本信息

批准号：
60480448
负责人：
SAKUDA Mamoru
金额：
$ 4.35万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

项目摘要

The purpose of this study was to find out whether allogeneic bone grafts can repairthe alveolar cleft instead of autogenous bone grafts. The followings are the steps carried in the experiment 1. An animal model for maxillary alveolar clefts using rats.For operative procedures, an incision was made through the tissues of the palate and the oral mucosa was raised to expose the premaxilla. A circular hole was cut using a trephane in a dental handpiece.One hole with diameter of 2mm was prepared in the midline of premaxilla just posterior to the upper incisors. The defects were allowed to fill with blood clot , and overlying soft tissues were repaired. Postoperatively, the animals continued to feed satifactorily. No bony healing was seen in the cavities radiographically and histologically on 35 days-postoperation.2. Bone induction through the demineralized bone matrices in an animal model for maxillary alveolar clefts. Allogeneic bone grafts were placed into the maxillary alveolar clefts in … More rats using demineralized bone powder prepared from long bone of rats according to Reddi et al. New bone formation was seen in the cavities radiographically and histologically, on 21 and 35 days-postoperation.3. Bone induction of demineralized bone matrices stimulated by electromagnetic fields using an animal model for maxillary alveolar clefts in rats. Rats with allogeneic bone grafts into maxillary alveolar cleft were exposed to pulsed electric magnetic fields (PEMFs which were generated by pulsating currents in the coils of a 1 msec burst of opposite polarity repeating at 15 Hz. Pallets of bone grafts in rats exposed to PEMFs were stimulated on alkaline phosphatase activity, <^(45)Ca> incorporation.It was demonstrated that bone inductive activity of demineralized bone matrices were stimulated by electromagnetic fields. Therefore, we conclude that clinically we may be able to use allogeneic bone grafts stimulated by electromagnetic fields into maxillary alveolar clefts instead of autogenous bone grafts. Less

本研究旨在探讨同种异体骨移植能否代替自体骨移植修复牙槽突裂。以下是在实验1中进行的步骤。本研究以大白鼠为实验动物，制作上颌骨齿槽裂的动物模型。在牙科手机上用环锯在上切牙后的前颌骨中线上钻一个直径为2mm的圆孔。允许缺损填充血凝块，并修复覆盖的软组织。术后，动物继续进食。结果：1.术后35天，X线片及组织学检查均未见骨愈合。脱钙骨基质诱导成骨治疗上颌骨牙槽嵴裂的实验研究。同种异体骨移植物被放置到上颌牙槽裂， ...更多信息大鼠用Reddi等从大鼠长骨制备的脱钙骨粉。术后21和35天，X线和组织学检查可见新骨形成。电磁场刺激脱矿骨基质诱导成骨的实验研究。将同种异体骨移植到上颌骨牙槽裂中的大鼠暴露于脉冲电磁场（PEMF，其由线圈中以15 Hz重复的1 msec相反极性脉冲串的脉冲电流产生。将大鼠骨移植物置于PEMFs中，观察到碱性磷酸酶活性和<^（45）Ca>掺入，证明电磁场可刺激脱钙骨基质的骨诱导活性。因此，我们得出结论，临床上，我们可能能够使用同种异体骨移植的电磁场刺激到上颌牙槽裂，而不是自体骨移植。少