Effect of Mechanical Stresses on Bone Remodeling

机械应力对骨重塑的影响

• 批准号: 03454483
• 负责人: SAKUDA Mamoru
• 金额: $ 4.29万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454483/
• 关键词: DNA synthesis; Centrifugal Force; Prostaglandins; Osteoblast; Mechanical Stress; Bone Remodeling; Alkaline Phosphatase; MC3T3-E1; MC3T3‐E1; アルカリフォスファタ-ゼ活性; プロスタグランデイン

The effect of biomechanical force on growth of skeletal tissue was studied in monolayer cultures of mouse osteoblastic MC3T3-E1 cells which were centrifuged at 320 g for 15 min to 72 h in a CO_2 incubator.Centrifugation of the cells for 30 min in low concentrations (0.3 or 1%) of fetal bovine serum (FBS) caused a two-fold increase of [^3H]thymidine incorporation at 20 h from commencing the centrifugation. However, centrifugation under 10% FBS caused no increase in [^3H]thymidine incorporation into DNA. Under 0.3% FBS,[^3H]thymidine incorporation increased in a manner dependent on the period of centrifugation and reached a maximum when the cells were centrifuged for 3 h. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. Moreover, conditioned medium collected from the centrifuged cultures increased [^3H]thymidine incorporation by two-fold over the basal when added to a quiescent culture of MC3T3-E1 cells. These results suggest that centrifugal force stimulates growth of osteoblastic cells through autocrine secretion of some diffusible growth-promoting activity.On the other hand, centrifugation of the cells inhibited induction by FBS of alkaline phosphatase activity and calcium-uptake, two indices of the differentiated phenotype of osteoblasts.

本实验以小鼠成骨细胞MC 3 T3-E1为材料，在CO_2培养箱中以320 g离心15 min ~ 72 h，研究生物力学力对骨骼组织生长的影响在离心开始后20小时，加入0.3%或1%胎牛血清（FBS）可使[^3H]胸苷掺入量增加2倍。然而，在10%FBS下离心并没有导致[^3H]胸苷掺入DNA的增加。在0.3%胎牛血清中，[^3H]胸苷掺入量随离心时间的延长而增加，离心3 h时达到最大值。在存在蛋白激酶C抑制剂H-7的情况下，离心对DNA合成的刺激被消除。此外，从离心培养物中收集的条件培养基添加到静止培养的MC 3 T3-E1细胞中时，[^3H]胸苷掺入比基础培养基增加了两倍。这些结果表明，离心力通过自分泌某些可扩散的促生长活性刺激成骨细胞的生长，另一方面，离心的细胞抑制FBS诱导的碱性磷酸酶活性和钙摄取，这两个成骨细胞分化表型的指标。

项目成果

N.INOUE,J.KATO,M.SAKUDA M.IWAMOTO,F.SUZUKI,Y.KATO: "Fundamentals of Bone Growth:Methodology and Applications" CRC PRESS INC., 626 (1991)

N.INOUE、J.KATO、M.SAKUDA M.IWAMOTO、F.SUZUKI、Y.KATO：“骨生长的基础：方法论和应用” CRC PRESS INC.，626 (1991)

開 祐司、井上 博之、鈴木 不仁男,田中 秀穂、近藤 淳、上園 昭人: "軟骨細胞の増殖分化機能を制御する軟骨特異的マトリックスChondromodulinーlその精製と分子クロ-ニング" 日本骨代謝学会雑誌. 9. 238-246 (1991)

Yuji Kai、Hiroyuki Inoue、Fujio Suzuki、Hideho Tanaka、Jun Kondo、Akito Uezono：“软骨调节蛋白的纯化和分子克隆，一种控制软骨细胞增殖和分化功能的软骨特异性基质”日本骨代谢学会杂志 9。 238-246 (1991)

H.INOUE,J.KATO,M.SAKUDA,M.IWAMOTO,F.SUZUKI,Y.KATO: "Fundamentals of Bone Growth:Methodology and Applications" CRC PRESS INC., 626 (1991)

H.INOUE、J.KATO、M.SAKUDA、M.IWAMOTO、F.SUZUKI、Y.KATO：“骨生长的基础：方法论和应用” CRC PRESS INC.，626 (1991)

Y.HIRAKI, H.INOUE, C.SHIGENO, Y.SANMA, H.BENTZ, D.M.ROSEN, A.ASADA, F.SUZUKI: "Bone Morphogenetic Proteins (BMP-2 and BMP-3) Promote Growth and Expression of the Differentiated Phenotype of Rabbit Chondrocytes and Osteoblastic MC3T3-E1 Cells In Vitro." J.

Y.HIRAKI、H.INOUE、C.SHIGENO、Y.SANMA、H.BENTZ、D.M.ROSEN、A.ASADA、F.SUZUKI：“骨形态发生蛋白（BMP-2 和 BMP-3）促进骨形态发生蛋白（BMP-2 和 BMP-3）的生长和表达

Y.HIRAKI, H.TANAKA, H.INOUE, J.KONDO, A.KAMIZONO, F.SUZUKI: "Molecular cloning of a new class of cartilage-specific matrix, chondromodulin-1, which stimulates growth of cultured chondrocytes." Biochem.Biophys.Res.Commun.175. 971-977 (1991)

Y.HIRAKI、H.TANAKA、H.INOUE、J.KONDO、A.KAMIZONO、F.SUZUKI：“新型软骨特异性基质 chondromodulin-1 的分子克隆，可刺激培养软骨细胞的生长。”