An investigation of bone induction by Growth Differentiation Factor-5/β-TCP complex in the dog frontal sinus and bone formation around the dental implants that were placed in.

本研究评估了使用β-磷酸三钙蛋白（β-TCP）基质与重组人生长分化因子5（rhGDF-5）诱导成年比格犬上颌窦底增强的骨形成的功效和技术可行性。项目1：β-TCP基质与rhGDF-5（rhGDF-5/β-TCP）的复合物由约300微米的β-TCP颗粒和含有rhGDF-5的rhGDF-5制成。每克 β-TCP 颗粒含有 500 微米颗粒。暴露双侧额窦，其中一个窦接受 2 克 rhGDF-5/β-TCP 植入物，另一个窦接受 2 克单独的 β-TCP 颗粒（对照）。手术后 1、2、4、8、12、16、20 和 24 周切除额窦，并通过组织学评估对 rhGDF-5/β-TCP 植入物的骨形成反应。植入后4周，在植入rhGDF-5/β-TCP的部位，在口骨表面的局部部位出现了新的松质骨和一些相互连接的小梁结构。 8 周时，在 rhGDF-5/β-TCP 植入物的窦内发现骨髓。在任何区域都没有发现软骨，并且在中间区域看到载体β-TCP的残留物。在对照植入物的位置，大部分植入的 β-TCP 颗粒保留在窦内。 12周时，大的新骨小梁延伸至rhGDF-5/β-TCP植入部位中心的原始骨表面。第 24 周时，所有可见的 β-TCP 颗粒均嵌入植入 rhGDF-5/β-TCP 部位的新形成骨中。相反，对照部位愈合24周后发现或多或少的β-TCP颗粒。 项目2：在含有rhGDF-5/β-TCP的部位放置钛合金（Ti-6A1-4V）种植体，术后4周移植。在种植体植入手术后 4、8 和 12 周的三个随访日期中的每一天，处死狗并对种植体周围的骨形成进行组织学评估。种植手术后8周，种植体螺纹之间出现新形成的骨，并将种植体表面与原始骨和β-TCP颗粒连接起来。相比之下，在对照部位没有骨与种植体表面接触的证据。 12周时，骨接触率增加并超过8周区域。较少的

This study assessed the efficacy and technical feasibility of inducing bone formation in adult beagle dogs of maxillary sinus floor augmention using β-tricalciuin phosphate (β-TCP) matrix with a recombinant human growth differentiation factor 5 (rhGDF-5).Project 1: The complex of the β-TCP matrix with rhGDF-5 (rhGDF-5/β-TCP) is made of approximately 300 micro-meter β-TCP particles and rhGDF-5 which contained 500 micro-grain per gram of β-TCP particles. Exposure of the frontal sinus was carried out bilaterally, with one sinus receiving 2 grams of the rhGDF-5/β-TCP implant and the other receiving 2 grams of β-TCP particles alone (control). The frontal sinuses were excised 1, 2, 4, 8, 12, 16, 20 and 24 weeks after surgery, and bone formation in response to the rhGDF-5/β-TCP implant was evaluated histologically. At 4 weeks of post-implantation in the site implanted with rhGDF-5/β-TCP, new cancellous bone and a little interconnected trabecular structure appeared at localized site on the ori … More ginal bone surface. At 8 weeks, bone marrow was found within the sinus of the rhGDF-5/β-TCP implants. Cartilage was not found in any region and residues of carrier β-TCP were seen in the middle area. At the site of the control implants, the bulk of implanted β-TCP particles remained within the sinus. At 12 weeks, the large new trabecular bone extended to the original bone surfaces in the center of the implant site of the rhGDF-5/β-TCP. At 24 weeks, all visible β-TCP particles were embedded in newly formed bone in the site implanted with rhGDF-5/β-TCP. On the contrary, more or less β-TCP particles were found after 24 weeks of healing in the control site.Project 2: The titanium alloy (Ti-6A1-4V) implants were placed in the site with rhGDF-5/β-TCP, which were grafted at 4 weeks after surgery. On each of three follow-up dates, which were 4, 8 and 12 weeks after implant placement surgery, the dogs were sacrificed and bone formations around the implant were evaluated histologically. 8 weeks after the implant placement surgery, newly formed bone was present between the implant threads and connected the implant surface with the original bone and β-TCP particles. In contrast, there was no evidence of bone contact with the implant surface at the control site. At 12 weeks, the bone contact ratio increased and it had surpassed the 8 weeks region. Less