An investigation of bone induction by Growth Differentiation Factor-5/β-TCP complex in the dog frontal sinus and bone formation around the dental implants that were placed in.

本研究采用重组人生长分化因子5 （rhGDF-5）结合β-磷酸三钙素（β-TCP）基质诱导成年beagle犬上颌窦底增强成骨的效果和技术可行性。项目1:β-TCP基质与rhGDF-5的复合物（rhGDF-5/β-TCP）由约300微米的β-TCP颗粒和每克含有500微米颗粒的rhGDF-5组成。双侧额窦暴露，一侧鼻窦接受2克rhGDF-5/β-TCP植入物，另一侧鼻窦单独接受2克β-TCP颗粒（对照）。术后1、2、4、8、12、16、20和24周切除额窦，组织学评价rhGDF-5/β-TCP植入物对骨形成的影响。植入rhGDF-5/β-TCP后4周，骨表面局部出现新的松质骨和少量相互连接的骨小梁结构。8周时，在rhGDF-5/β-TCP植入物的窦内发现骨髓。任何区域均未发现软骨，中间区域可见载体β-TCP残基。在对照植入物的位置，植入的β-TCP颗粒大部分留在窦内。12周时，大的新生骨小梁延伸至rhGDF-5/β-TCP种植体中心的原骨表面。在24周时，所有可见的β-TCP颗粒都嵌入到rhGDF-5/β-TCP植入部位的新骨中。相反，治疗24周后，对照部位出现或多或少的β-TCP颗粒。项目二：采用rhGDF-5/β-TCP植入部位的钛合金（Ti-6A1-4V）种植体，于术后4周进行移植。在植入手术后4周、8周和12周的三个随访日期中，狗被处死，并对植入物周围的骨形成进行组织学评估。植入种植体8周后，种植体螺纹之间出现新生骨，将种植体表面与原骨及β-TCP颗粒连接。相比之下，在对照部位没有骨与种植体表面接触的证据。12周时，骨接触率增加，已超过8周区域。少

This study assessed the efficacy and technical feasibility of inducing bone formation in adult beagle dogs of maxillary sinus floor augmention using β-tricalciuin phosphate (β-TCP) matrix with a recombinant human growth differentiation factor 5 (rhGDF-5).Project 1: The complex of the β-TCP matrix with rhGDF-5 (rhGDF-5/β-TCP) is made of approximately 300 micro-meter β-TCP particles and rhGDF-5 which contained 500 micro-grain per gram of β-TCP particles. Exposure of the frontal sinus was carried out bilaterally, with one sinus receiving 2 grams of the rhGDF-5/β-TCP implant and the other receiving 2 grams of β-TCP particles alone (control). The frontal sinuses were excised 1, 2, 4, 8, 12, 16, 20 and 24 weeks after surgery, and bone formation in response to the rhGDF-5/β-TCP implant was evaluated histologically. At 4 weeks of post-implantation in the site implanted with rhGDF-5/β-TCP, new cancellous bone and a little interconnected trabecular structure appeared at localized site on the ori … More ginal bone surface. At 8 weeks, bone marrow was found within the sinus of the rhGDF-5/β-TCP implants. Cartilage was not found in any region and residues of carrier β-TCP were seen in the middle area. At the site of the control implants, the bulk of implanted β-TCP particles remained within the sinus. At 12 weeks, the large new trabecular bone extended to the original bone surfaces in the center of the implant site of the rhGDF-5/β-TCP. At 24 weeks, all visible β-TCP particles were embedded in newly formed bone in the site implanted with rhGDF-5/β-TCP. On the contrary, more or less β-TCP particles were found after 24 weeks of healing in the control site.Project 2: The titanium alloy (Ti-6A1-4V) implants were placed in the site with rhGDF-5/β-TCP, which were grafted at 4 weeks after surgery. On each of three follow-up dates, which were 4, 8 and 12 weeks after implant placement surgery, the dogs were sacrificed and bone formations around the implant were evaluated histologically. 8 weeks after the implant placement surgery, newly formed bone was present between the implant threads and connected the implant surface with the original bone and β-TCP particles. In contrast, there was no evidence of bone contact with the implant surface at the control site. At 12 weeks, the bone contact ratio increased and it had surpassed the 8 weeks region. Less