Structure Prediction of Intrinsic Membrane Proteins

内在膜蛋白的结构预测

• 批准号: 61460248
• 负责人: MITAKU Shigeki
• 金额: $ 2.88万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61460248/
• 关键词: Biological membrane; Membrane protein; Protein structure; Structure Prediction; Denaturation; Hydrophobic Interaction; 水素結合

Membrane proteins have many important biological functions. However, their structure is hardly known. Therefore, the aim of this research project is to clarify the mechanism of structure formation of membrane proteins and to devise a method for the structure prediction. The results of this research may be summarized as follows. (1) The comparison between the amino acid sequences of soluble and membrane proteins revealed that the information of hydrophobicicity is enough for the distinction between the two types of proteins. (2) Transmembrane helices is bound with each other by hydrogen bonds, as inferred from denaturation experiments. Namely, spectroscopic measurements showed that alcohols destroyed the tertiary structure of bacteriorhodopsin, a typical membrane protein, and that hydrogen bonds were specifically destroyed. (3) The distribution of hydrogen bonding residues in the amino acid sequences of membrane proteins having seven transmembrane helices was compatible with the hydrogen bonding mechanism of the tertiary structure formation. Consequently, the principle of the structure prediction of membrane proteins has become clear by this research project.

膜蛋白具有许多重要的生物学功能。然而，其结构几乎不为人知。因此，本研究的目的是阐明膜蛋白结构形成的机制，并设计一种结构预测的方法。这项研究的结果可归纳如下。(1)通过比较可溶性蛋白和膜蛋白的氨基酸序列，发现疏水性的信息足以区分这两类蛋白。(2)从变性实验中可以推断，跨膜螺旋通过氢键相互结合。也就是说，光谱测量表明，醇类破坏了细菌视紫红质（一种典型的膜蛋白）的三级结构，并且氢键被特异性地破坏。(3)具有7个跨膜螺旋的膜蛋白的氨基酸序列中的氢键残基的分布与三级结构形成的氢键机制是相容的。因此，通过本研究项目，明确了膜蛋白结构预测的原理。

项目成果

美宅成樹: 化学と生物. 25. 820-823 (1987)

N. Miyake：化学与生物学。25. 820-823 (1987)

S. Mitaku; T. Sakka; T. Hotta: "Ultrasonic Measurement of Melittin-Liposome System" Japan. J. Appl. Phys.27-1. 44-46 (1988)

S.Mitaku；

美宅成樹: "バイオ.高分子研究の進歩 第2巻 「生体膜複合体と合成膜の機能デザイン」" 学会出版センター, 200 (1987)

三宅茂树：“生物聚合物研究进展第2卷‘生物膜复合物和合成膜的功能设计’”学会出版中心，200（1987）

美宅成樹: 生物物理学会誌. 28. 317-322 (1988)

Miyake, N.：《生物物理学会杂志》28. 317-322 (1988)。

美宅成樹,幾田一哉,柳原憲那,諏訪牧子: "蛋白質核酸酵素増刊号「視覚の分子メカニズム」「膜タンパク質における立体構造の予測法」" 共立出版,

Naruki Miyake、Kazuya Ikuta、Kenna Yanagihara、Makiko Suwa：“蛋白质核酸酶特刊：‘视觉的分子机制’、‘膜蛋白三维结构的预测方法’”Kyoritsu Shuppan，