Microorganisms in the capillary fringe, rapidly changing environment

毛细血管边缘的微生物，快速变化的环境

• 批准号: 43741788
• 负责人: Professorin Dr. Claudia Gallert
• 金额: --
• 依托单位: Institut für Ingenieurbiologie und Biotechnologie des Abwassers (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/43741788?language=en
• 关键词: Microorganisms; capillary; fringe; rapidly; changing

The influence of suspended or immobilized, biofilms forming microorganisms in capillary fringes (CF´s) on the physical behaviour (e.g. changes of hydraulic parameters, extent of the CF, water content) and changes of bio- or geochemistry in the soil-water-gas-interphase will be investigated in cooperation with water chemists (Frimmel/Abbt-Braun), geologists (Grathwohl), (soil) physicists (Vogel/Geistlinger) and mathematicians (Bastian/Ippisch) for modeling of experimental data. The lab work will include short term and long term experiments to elucidate pore size changes (bioclogging) by biofilm formation, capillary suction at mineralic/organic surfaces, changes in soil water content in the presence/absence of extracellular polymeric substances (EPS) or biosurfactants and bacterial movement. A technique to visualize and estimate (quantify) the bacterial distribution, activity and biofilm formation in a variably saturated porous medium (CF) by utilization of fluorescent substrates and natural or modified fluorescent bacteria with non-invasive techniques will be developed. The influence of suspended or immobilized bacteria in the CF on transport processes of oxygen coupled with biochemical reactions will be investigated and data will be used to model and predict the fate of a pollutant at fluctuating water tables and under different nutrition levels. When nitrate is used as electron acceptor subsequent nitrogen gas formation occurs during denitrification and the influence of gas bubble formation and disappearance on transport processes will be investigated.

将与水化学家（Frimmel/ abbot - braun）、地质学家（Grathwohl）、（土壤）物理学家（Vogel/Geistlinger）和数学家（Bastian/Ippisch）合作，研究悬浮或固定的生物膜在毛细管条纹（CF）中形成的微生物对物理行为（例如水力参数的变化、CF的范围、含水量）和土壤-水-气界面生物或地球化学变化的影响，以建立实验数据模型。实验室工作将包括短期和长期实验，以阐明通过生物膜形成的孔隙大小变化（生物堵塞），矿物/有机表面的毛细管吸力，存在/不存在细胞外聚合物质（EPS）或生物表面活性剂和细菌运动时土壤含水量的变化。利用荧光底物和天然或修饰的荧光细菌与非侵入性技术，将开发一种在可变饱和多孔介质（CF）中可视化和估计（量化）细菌分布、活性和生物膜形成的技术。将研究CF中悬浮或固定化细菌对氧运输过程以及生化反应的影响，并利用数据模拟和预测在波动的地下水位和不同营养水平下污染物的命运。当硝酸盐作为电子受体时，反硝化过程中会产生后续的氮气，并研究气泡的形成和消失对输运过程的影响。