权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The properties and regulations of proteins to form cytoskeletons in higher plants

高等植物细胞骨架蛋白质的性质和调控

基本信息

批准号：
61480012
负责人：
MIZUNO Koichi
金额：
$ 4.03万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

We have studied about the properties and functions of cytoskeletal elements and its protein compositions in higher plants.Cultured tobacco cells possess minor tubulin-like protein,which is immunologically classified as <alpha>-tubulin. This protein induced the depolymerization and the inhibition of polymerization of microtubules.Immunofluorescent studies using antibodies against tubulin often showed the microtubules protruding from the surface of nuclei to cytoplasm and sometimes to inside of nuclei just like the initiation of spindle microtubules formation.This observation suggested the localization of MTOC-like substances on nuclei.In fact,MTOC-like active substance(s)was prepared from nuclei of higher plants.In vitro polymerizable actin was isolated from hifer plant cells.The biochemical properties would be shown within short time.We found three novel cytoskeletal elements from higher plant cells.They seems to be unique cytoskeletons in higher plants,therefore,the regulation systems … More will be interest.50 kd protein polymerizad intermediate filament-like structures in the presence of guanosine nucleotides.This protein was shown to be basic protein and cross-reacted with antitubulin antibodies.Monoclonal antibodies showed the localization of 50 kd protein around the nuclei and cortical region as network in interphase cells.Phragmoplast was also shown to contain this protein.58 kd protein was purified from insoluble fraction in the presence of ATP,this protein readily solubilized by the depletion of ATP.Addition of ATP induced the reversible polymerization and formed fibrillar bundles of thin filament having a diameter about 3[5 nm.Monoclonal antibodies showed the distribution of this bundles around the surface of nuclei protruding to plasma membrane. Possible involvement of the fibrillar bundles in the mechanism to determine the site at which cytokinesis will occur and to move the nucleus in relation to the division site.100 kd protein was solubilized with KC1 fron insoluble fraction and purified.This protein polymerized double chain filaments haying a diameter about 15 nm in vitro.The function of these cytoskeletal element should be further studied. Less

我们对高等植物细胞骨架成分及其蛋白质组成的性质和功能进行了研究。培养的烟草细胞含有微管蛋白样蛋白，免疫学上将其归类为<α-微管蛋白。该蛋白诱导微管解聚并抑制聚合。使用针对微管蛋白的抗体进行的免疫荧光研究经常显示微管从细胞核表面突出到细胞质，有时突出到细胞核内部，就像纺锤体微管形成的起始一样。这一观察表明MTOC样物质定位在细胞核上。事实上，类似MTOC的活性物质是从高等植物的细胞核中制备出来的。体外可聚合的肌动蛋白是从海马植物细胞中分离出来的。其生化特性会在短时间内表现出来。我们从高等植物细胞中发现了三种新颖的细胞骨架元件。它们似乎是高等植物中独特的细胞骨架，因此，调控系统......更多将受到关注。50 kd蛋白聚合在鸟苷核苷酸存在的情况下，形成中间丝状结构。该蛋白质被证明是碱性蛋白质，并与抗微管蛋白抗体发生交叉反应。单克隆抗体显示 50 kd 蛋白质定位在细胞核和皮质区域周围，在间期细胞中呈网络状。Phragmoplast 也被证明含有这种蛋白质。58 kd 蛋白质是从不溶性物质中纯化出来的在存在 ATP 的情况下，该蛋白质很容易因 ATP 的消耗而溶解。添加 ATP 诱导可逆聚合并形成直径约为 3[5 nm] 的细丝纤维束。单克隆抗体显示该纤维束分布在突出到质膜的细胞核表面周围。纤维束可能参与确定胞质分裂发生位点以及相对于分裂位点移动细胞核的机制。用KC1不溶组分溶解并纯化100 kd的蛋白质。该蛋白质在体外聚合双链丝，直径约为15 nm。这些细胞骨架元件的功能应进一步研究。较少的