Function of ABP195 member of a new small 'superfamily' of plant actin binding proteins; involvement in actin organisation and signalling

植物肌动蛋白结合蛋白新小“超家族”ABP195成员的功能；

• 批准号: BB/G006334/1
• 负责人: P Hussey
• 金额: $ 41.12万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2009
• 资助国家: 英国
• 起止时间: 2009 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FG006334%2F1
• 关键词: Function; ABP195; member; new; small

Plants are sedentary and have to respond to unique environmental and developmental signals in order to survive. The actin cytoskeleton is essential for life, is stimulus responsive and reorganises so that cells can divide and expand, generate organelle movement, polarise cell growth and thicken the cell wall; events that are required for healthy plant development i.e. for proliferation and growth, for maximising energy production, for the uptake of nutrients, for managing the transpiration stream, for reproduction and for protection against pathogen invasion. In order to perform these roles the actin network has to be able to respond to signals and reorganise, processes that are coordinated by actin associated proteins that anchor, crosslink or otherwise regulate this network. With its unique capabilities the actin cytoskeleton has become adapted to plant specific function by (1) tuning the function of its associated proteins, common to animals, fungae and plants, for its own purposes; there is a wealth of evidence of this from work in my lab and others and (2) the evolution of a set of plant specific associated proteins whose function on the actin network is unique to plants. This programme of research concerns a family of proteins that fit into category (2). The first indication that we had a new class of actin binding proteins in plants came from a screen for proteins localising to different compartments within a plant cell. This screen was performed by Oparka and colleagues (University of Edinburgh) and it revealed many new proteins locating to compartments such as Golgi, mitochondria, nucleus, plastids and the cytoskeleton. We have shown, as supporting data for this programme, that one of the proteins localising to the cytoskeleton localised to actin filaments. Identification of the actin binding domain in the amino -terminal region allowed us to identify 14 other proteins having the same domain but that the 15 proteins could be divided into 5 groups based on there carboxy-terminal sequence. This is not unlike the situation for known 'Superfamilies' of actin binding proteins where a common actin binding domain is attached to unique sequence and this unique sequence specifies its role with respect to actin in the cell. Preliminary data we present here plus utilisation of data from current databases suggest that one member of this superfamily is involved with the organisation of the actin network at the plasma membrane and one member is essential for normal plant development. The goal of this research is to understand how these two proteins contribute to the normal function of the actin cytoskeleton in plant development.

植物是久坐不动的，为了生存，必须对独特的环境和发育信号做出反应。肌动蛋白细胞骨架是生命所必需的，是对刺激的反应和重组，使细胞可以分裂和扩张，产生细胞器运动，极化细胞生长和加厚细胞壁；植物健康发育所需的事件，如增殖和生长，最大限度地产生能量，吸收营养物质，管理蒸腾气流，繁殖和保护免受病原体入侵。为了发挥这些作用，肌动蛋白网络必须能够对信号做出反应，并重组由锚定、交联或以其他方式调节该网络的肌动蛋白相关蛋白协调的过程。由于其独特的能力，肌动蛋白细胞骨架通过(1)调节其相关蛋白的功能(动物、真菌和植物常见的)来适应植物的特定功能；从我的实验室和其他实验室的工作中有大量的证据表明这一点；(2)一组植物特有的相关蛋白的进化，其在肌动蛋白网络上的功能是植物所独有的。这项研究计划涉及一类属于(2)的蛋白质。我们在植物中发现了一类新的肌动蛋白结合蛋白的第一个迹象来自于对定位于植物细胞内不同隔室的蛋白质的筛选。这一筛选是由Oparka和他的同事(爱丁堡大学)进行的，它揭示了许多新的蛋白质定位于高尔基体、线粒体、细胞核、质体和细胞骨架。我们已经证明，作为这个项目的支持数据，定位于细胞骨架的蛋白质之一定位于肌动蛋白细丝。通过对氨基末端肌动蛋白结合域的鉴定，我们可以鉴定出另外14个具有相同结构域的蛋白质，但根据它们的羧基末端序列可以将这15个蛋白质分成5个组。这与已知的肌动蛋白结合蛋白超家族的情况没有什么不同，其中共同的肌动蛋白结合域连接到唯一的序列，并且这个独特的序列指定了它在细胞中相对于肌动蛋白的作用。我们在这里提供的初步数据加上利用现有数据库的数据表明，这个超家族中的一个成员参与了质膜肌动蛋白网络的组织，一个成员对正常的植物发育是必不可少的。这项研究的目的是了解这两种蛋白质如何在植物发育过程中对肌动蛋白细胞骨架的正常功能做出贡献。

项目成果

Membrane contact sites and cytoskeleton-membrane interactions in autophagy.

• DOI: 10.1002/1873-3468.14414
• 发表时间: 2022-09
• 期刊: FEBS letters
• 影响因子: 3.5
• 作者: Duckney PJ;Wang P;Hussey PJ
• 通讯作者: Hussey PJ

NETWORKED 3B: a novel protein in the actin cytoskeleton-endoplasmic reticulum interaction.

• DOI: 10.1093/jxb/erx047
• 发表时间: 2017-03-01
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Wang P;Hussey PJ
• 通讯作者: Hussey PJ

Interactions between plant endomembrane systems and the actin cytoskeleton.

• DOI: 10.3389/fpls.2015.00422
• 发表时间: 2015
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Wang P;Hussey PJ
• 通讯作者: Hussey PJ

Blobs and curves: object-based colocalisation for plant cells.

• DOI: 10.1071/fp14047
• 发表时间: 2015-05
• 期刊: Functional plant biology : FPB
• 作者: Carl J. Nelson;Patrick J Duckney;Timothy J. Hawkins;M. Deeks;P. Laissue;P. Hussey;B. Obara