Studies of Rh antigen-related gene (RNA and DNA) and its Medico-Legal application

Rh抗原相关基因（RNA和DNA）的研究及其医疗法律应用

• 批准号: 61480176
• 负责人: FUKUSHIMA Hirofumi
• 金额: $ 4.03万
• 依托单位: Dept. of Legal Medicine, Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480176/
• 关键词: DNA analysis; mRNA; Rh antigen; LW antigen; RIA; ELISA; クローニング; DNA解析; Lh抗原

Enzyme-linked immumosorbent assay (ELISA) was used for ABO,Rh,fy,Jk,K Blood grouping. Positive and negatine reactions of anti-A, anti-B, anti-D, anti-C, anti-Fya, anti-Jka, and anti-K wells were ensily distinguished by this method. As pant of a study of frozm red cells, we tested the stability of the ABO and Rh antigens in frozem ulls. There were no significant changes of 125I-proteinA binding counts. There appear to be no barriers to the use of frozem uellss for fomily study or Rh blood type antigens. In order to isolate mRNA which is related to Rh 25(LW) antigen, we are now trying to purify total RNA from Japanese monky spleen. To obtain mRNA from exuss of other RNA, we are going to separate the RNA by chronatography on an oligo-(dT)-cellulose column.

采用酶联免疫吸附试验（ELISA）进行ABO、Rh、Fy、Jk、K血型鉴定。该方法可灵敏地区分抗A、抗B、抗D、抗C、抗Fya、抗Jka和抗K威尔斯孔的阳性反应和阴性反应。作为冻存红细胞研究的一部分，我们对冻存红细胞中ABO和Rh抗原的稳定性进行了检测。125 I-蛋白A结合计数无明显变化。使用冻存抗原进行家族研究或Rh血型抗原似乎没有障碍。为了分离与Rh 25（LW）抗原相关的mRNA，我们试图从日本猴脾脏中纯化总RNA。为了从其他RNA的外显子中获得mRNA，我们将在寡聚（dT）纤维素柱上通过计时法分离RNA。

项目成果

Hirofumi Fukushima: Jpn J Legal Med. 40. 366-369 (1986)

福岛博文：Jpn J Legal Med。

Hirofumi Fukushima: Act. Crim. Japon. 53. 244-247 (1987)

福岛博文：行动。

Hirofumi,Fukushima: "Localization of Rh 1(D),2(C),3(E),4(c),5(E) and 25(LW) antigens of humar Rh blood groups in fetal erythrocyte membranes" Comp. Buichem. Physial.86B. 303-305 (1987)

Hirofumi，Fukushima：“胎儿红细胞膜中人类 Rh 血型的 Rh 1(D)、2(C)、3(E)、4(c)、5(E) 和 25(LW) 抗原的定位”比较。

Hirofumi Fukushima: J. Forens. Sa. 32. 531-534 (1987)

福岛博文：J. Forens。

Naoko Misawa: Act. Crim. Japon. 53. 153-157 (1987)

三泽直子：行动。