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Studies of Rh antigen-related gene (RNA and DNA) and its Medico-Legal application

Studies of Rh antigen-related gene (RNA and DNA) and its Medico-Legal application
Rh抗原相关基因(RNA和DNA)的研究及其医疗法律应用
批准号:
61480176
负责人:
FUKUSHIMA Hirofumi
金额:
$4.03万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1986
资助国家:
日本
项目状态:
已结题
起止时间:
1986 至 1987

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中文摘要
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英文摘要
Enzyme-linked immumosorbent assay (ELISA) was used for ABO,Rh,fy,Jk,K Blood grouping. Positive and negatine reactions of anti-A, anti-B, anti-D, anti-C, anti-Fya, anti-Jka, and anti-K wells were ensily distinguished by this method. As pant of a study of frozm red cells, we tested the stability of the ABO and Rh antigens in frozem ulls. There were no significant changes of 125I-proteinA binding counts. There appear to be no barriers to the use of frozem uellss for fomily study or Rh blood type antigens. In order to isolate mRNA which is related to Rh 25(LW) antigen, we are now trying to purify total RNA from Japanese monky spleen. To obtain mRNA from exuss of other RNA, we are going to separate the RNA by chronatography on an oligo-(dT)-cellulose column.
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Hirofumi,Fukushima: "Localization of Rh 1(D),2(C),3(E),4(c),5(E) and 25(LW) antigens of humar Rh blood groups in fetal erythrocyte membranes" Comp. Buichem. Physial.86B. 303-305 (1987)
Hirofumi,Fukushima:“胎儿红细胞膜中人类 Rh 血型的 Rh 1(D)、2(C)、3(E)、4(c)、5(E) 和 25(LW) 抗原的定位”比较。
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16
    Study on mitochondrial heteroplasmy in related and unrelated individuals
    • 批准号:
      16590537
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.98万
    • 财政年份:
      2004
    • 负责人:
      FUKUSHIMA Hirofumi
    • 依托单位:
    Analysis of mitochondrial DNA control region and forensic applications
    • 批准号:
      13557036
    • 项目类别:
      Grant-in-Aid for Scientific Research (B)
    • 资助金额:
      $8.19万
    • 财政年份:
      2001
    • 负责人:
      FUKUSHIMA Hirofumi
    • 依托单位:
    Sudy on the heteroplasmic substitutions in the mitochondrial DNA control region
    • 批准号:
      13670419
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $0.77万
    • 财政年份:
      2001
    • 负责人:
      FUKUSHIMA Hirofumi
    • 依托单位:
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      10307011
    • 项目类别:
      Grant-in-Aid for Scientific Research (A).
    • 资助金额:
      $11.97万
    • 财政年份:
      1998
    • 负责人:
      FUKUSHIMA Hirofumi
    • 依托单位:
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    • 项目类别:
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    • 批准年份:
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