Study on the sequence and determination of the allelic variants at the D1S80 locus

D1S80位点等位基因变异的序列研究和测定

• 批准号: 10307011
• 负责人: FUKUSHIMA Hirofumi
• 金额: $ 11.97万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10307011/
• 关键词: PCR; subtyping; paternity testing; polymorphism; DNA; D1S80; sequence; variants; DIS80; 蛍光色素; 多型; DNA; 塩基配列

Through analyzing the alleles in Japanese and Chinese populations, we established a new nomenclature for the PCR-RFLP band patterns, closely relating them to their corresponding sequences so that the new types could be designated easily and accurately. The short fragments of 16 bp, 32 bp, 48 bp, 64 bp, and 80 bp contained 1,2,3,4, and 5 repeat units, respectively, and were thus named bands "1", "2", "3", "4", and "5". Excluding a flanking region, the eight kinds of long fragments of 221 bp, 237 bp, 253 bp, 269 bp, 285bp, 317 bp, 349bp, and 365 bp in length each contained 6,7,8,9,10,12,14, and 15 repeat units.To testify whether alleles of the same length and of the same band pattern possessed the same sequences, a number of alleles were sequenced in addition to the above-sequenced samples. Of the sequenced samples which had the same lengths and the same band patterns, most shared the same sequences. However, some revealed different sequences. Because of the differences in the repeat arrays between the variants and the ladder markers, structural differences or one-sided destribution of the electrical charge led to more apparent migration in polyacrylamide gel electrophoresis.

通过对日本人和中国人的等位基因分析，建立了新的PCR-RFLP带型命名法，并将其与相应的序列紧密联系起来，从而方便、准确地划分新的类型。16 bp、32 bp、48 bp、64 bp和80 bp的短片段分别含有1、2、3、4和5个重复单位，因此命名为条带“1”、“2”、“3”、“4”和“5”。除侧翼区外，221、237、253、269、285、317、349和365 bp的8种长片段分别含有6、7、8、9、10、12、14和15个重复单位。除了上述测序的样品外，还对许多等位基因进行测序。在长度和带型相同的测序样品中，大多数具有相同的序列。然而，有些人揭示了不同的序列。由于变异体和梯状标记之间重复序列的差异，结构差异或电荷的单侧偏置导致聚丙烯酰胺凝胶电泳中更明显的迁移。

项目成果

K.Takayanagi: "Mitochondrial DNA analysis : Identification"Res.Pract.Forens.Med.. 42. 87-90 (1999)

K.Takayanagi：“线粒体 DNA 分析：鉴定”Res.Pract.Forens.Med.. 42. 87-90 (1999)

Takayanagi, K., Hanawa, K., Liu, C., Asamura, H., Ota, M., Fukushima, H.: "Mitochondrial DNA analysis : Identification performed using the shed hairs of two missing persons."Res.Pract Forens.Med.. 42. 87-90 (1999)

Takayanagi, K.、Hanawa, K.、Liu, C.、Asamura, H.、Ota, M.、Fukushima, H.：“线粒体 DNA 分析：使用两名失踪人员脱落的头发进行身份识别。”Res.Pract

Liu,C et al: "DIS80 subtyping by PCR-RFLP : new nomenclature and further characterization"Legal Med. 1. 210-216 (1999)

Liu,C 等人：“通过 PCR-RFLP 进行 DIS80 亚型分析：新命名法和进一步表征”Legal Med。

C.Liu: "Investigation of ACTBP2 mutations in the Japanese population"Legal Med. 2. 75-78 (2000)

C.Liu：“日本人群 ACTBP2 突变的调查”Legal Med。

Sano, K., Takayanagi, K., Kaneko, T., Liu, C., Kinoshita, T., Nakamura, N., Asamura, H., Ito, M., Fukushima, H., Hotchi, M.: "Application of short tandem repeat of genomic DNA and mitochondrial DNA for identification of mixed-up tissue specimens."Patholog

佐野 K.、高柳 K.、金子 T.、刘 C.、木下 T.、中村 N.、浅村 H.、伊藤 M.、福岛 H.、Hotchi, M.：