Analysis of mitochondrial DNA control region and forensic applications

线粒体 DNA 控制区分析及法医学应用

• 批准号: 13557036
• 负责人: FUKUSHIMA Hirofumi
• 金额: $ 8.19万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557036/
• 关键词: PCR; mitochondria; D loop; polymorphism; HV1; HV2; sequence; control region; HVI; HVII

The small size of the mitochondrial DNA and its presence in many copies per cell make it useful for human DNA tyring when only small amounts of material are available or when the material is badly degraded. The sequence information of the mtDNA hypervariable regions 1 and 2 (HV1 and HV2) provided a robust tool for identification purposes relevant for the forensic science.The mtDNA sequence were generated for 95 unrelated donors living in Japan. A total 91 different haplotypes were observed for HV1 and HV2 regions.We typed six mutation sites in the coding region by use of amplified produet - length polymorphism (APLP) analysis. Two of these haplotypes, B1 and C2 were distributed in Japanese population. Moreover, the haplotypes A1, A2, B2, B3, and C2, were mostly restricted to the Mongoloid populations.Personal identification were performed in 8 autopsy cases. Each cadaver was severely damaged, and neither fingerprint nor dental information could be obtained for personal identification. In all cases, it was possible to amplify and sequence the control regions, resulting in positive identification in 6 of 8 cases.

线粒体DNA的小尺寸及其在每个细胞中的许多拷贝的存在使其在只有少量材料可用或当材料严重降解时可用于人类DNA tyring。mtDNA高变区1和2（HV1和HV2）的序列信息为法医学相关的鉴定目的提供了一个强大的工具。在HV1和HV2区共观察到91种不同的单倍型，我们用扩增产物长度多态性（APLP）分析法对编码区的6个突变位点进行了分型。其中两种单倍型B1和C2分布于日本人群。单倍型A1、A2、B2、B3和C2主要分布于蒙古人种。每具尸体都严重受损，无法获取指纹和牙齿信息进行个人身份识别。在所有情况下，都可以扩增和测序对照区，导致8例中有6例阳性鉴定。

项目成果

Asamura H., Takayanagi K., Tsukada K., Ota M., Fukushima H.: "DNA analysis of die two pieces of bone founded in the mountains."DNA polymorphism. 11. 271-273 (2002)

Asamura H.、Takayanagi K.、Tsukada K.、Ota M.、Fukushima H.：“对山中发现的两块骨头进行 DNA 分析。”DNA 多态性。

Asamura H., Takayanagi K., Ota M., Kurita H., Kurashina K., Fukushima H.: "Personal Identification by Mitochondrial DNA Control Region: Cases of Severely Damaged Cadaver Lacking Dental and Fingerprint Information."Res Pract Forens Med. 45. 157-161 (2002)

Asamura H.、Takayanagi K.、Ota M.、Kurita H.、Kurashina K.、Fukushima H.：“通过线粒体 DNA 控制区域进行个人识别：缺乏牙齿和指纹信息的严重受损尸体案例。”Res Pract Forens Med。

浅村 英樹: "山中より発見された骨片のDNA分析"DNA多型. 10. 271-273 (2002)

浅村秀树：《山中发现的骨碎片的 DNA 分析》DNA 多态性。 10. 271-273 (2002)。

Takayanagi K., Asamura H., Tsukada K., Ota M., Saito S., Fukushima H.: "Investigation of DNA extraction from hair shafts."International Congress Series,. 1239. 759-764 (2003)

Takayanagi K.、Asamura H.、Tsukada K.、Ota M.、Saito S.、Fukushima H.：“从毛干中提取 DNA 的研究”。国际大会系列，。

Asamura H., Takayanagi K., Tsukada K., Ota M., Fukushima H.: "Comparison of DNA extraction methods from a small amount of bone."DNA polymorphism. in press (2003)

Asamura H.、Takayanagi K.、Tsukada K.、Ota M.、Fukushima H.：“从少量骨骼中提取 DNA 方法的比较”。DNA 多态性。