Study on the automated DNA profilling in paternity testing

亲子鉴定中DNA自动分析的研究

• 批准号: 08557034
• 负责人: FUKUSHIMA Hirofumi
• 金额: $ 3.26万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08557034/
• 关键词: PCR; fluorescent; paternity testing; STR; polymorphism; DNA; minisatellite; microsatellite; 電気泳動; 自動検出; 蛍光色素

Based on the length and genomic distribution of the core repeat sequences, two systems are classified as minisatellites and microsatellites (or short tandem repeats). In particular, short tandem repeats (STRs) are abundant classes of polymorphic loci dispersed throughout the entire genome. Each STR locus usually consists of a limited number of the core repeats of 2-5 basepairs, which can easily be resolved by analytical gel electrophoresis. The Japanese and Chinese populations were studies for the ACTBP2 and D4S43 loci. In combination with the use of fluorescence-labeled primers employing different fluorescent dyes, STR loci with overlapping or identical allele size ranges can be analyzed together in the same lane of polyacrylamide gel, thus providing a rapid and accurate method for paternity testing and human identification. Automated DNA profilling by fluorescence-based technology, as well as its application, have been studied and validated for use in paternity testing routine.

基于核心重复序列的长度和基因组分布，两个系统被分类为小卫星和微卫星（或短串联重复序列）。特别地，短串联重复序列（STR）是分布在整个基因组中的丰富的多态性基因座类别。每个STR基因座通常由有限数量的2-5个碱基对的核心重复序列组成，其可以容易地通过分析凝胶电泳来解析。对日本和中国人群进行了ACTBP 2和D4 S43基因座的研究。结合使用不同荧光染料的荧光标记引物，可以在同一条聚丙烯酰胺凝胶泳道上同时分析具有重叠或相同等位基因大小范围的STR基因座，从而为亲子鉴定和人类身份识别提供快速准确的方法。基于荧光技术的自动DNA分析及其应用已经被研究和验证用于亲子鉴定常规。

项目成果

Liu C: "ACTBP2 genefroquency distribution and sequencing of the allelic ladder and variant" Int J Legal Med. 110. 208-212 (1997)

Liu C：“ACTBP2 基因频率分布以及等位基因梯和变体的测序”Int J Legal Med。

Katsuyama Y: "Allele distribution of a VNTR polymorphism at D4S43 Locus in the Japanes population" Jpn J Legal Med. 50. 6-9 (1996)

Katsuyama Y：“日本人群中 D4S43 位点 VNTR 多态性的等位基因分布”Jpn J Legal Med。

Harashima, N., Ota, M., Katsuyama, Y., Sugiyama, E., Liu, C.Y., Fukushima, H.: "The allele frequency distribution at VNTR locus D17S5 (YNZ22) in a Japanese population sample." Jpn J Legal Med.50. 237-240 (1996)

Harashima, N.、Ota, M.、Katsuyama, Y.、Sugiyama, E.、Liu, C.Y.、Fukushima, H.：“日本人群样本中 VNTR 位点 D17S5 (YNZ22) 的等位基因频率分布。”

Saito, S., Ota, S., Hashizume, K., Yamada, E., Kaneshige, T., Kinoshita, T., Hashimoto, M., Oguchi, H., Ishii, E., Fukushima, H.: "A new HLA-DQB1^<**>0306 allele sharing motifs from DQB1^<**>03032 and DQB1^<**>04 sequences." Tissue Antigens. 48. 580-585 (

Saito, S.、Ota, S.、Hashizume, K.、Yamada, E.、Kaneshige, T.、Kinoshita, T.、Hashimoto, M.、Oguchi, H.、Ishii, E.、Fukushima, H.：

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