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Synaptic Inputs to Neurosecretory Neurons

神经分泌神经元的突触输入

基本信息

批准号：
61480482
负责人：
NAKAI Yasumitsu
金额：
$ 1.41万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

To examine synaptic inputs to neurosecretory neurons in the rat hypothalamus at the electron microscopic level, some double immunolabeling techniques and a combined technique of immunocytochemistry and autoradiography after 3H-noradrenaline (^3H-NA) or 5-hydroxydopamine (5-OHDA) labeling in the same tissue section have been used. Concerning the innervation of neurohypophyseal hormone-containing neurons, peroxidase-antiperoxidase (PAP) labeled tyrosine hydroxylase, GABA or serotonin immunoreactive terminals making synaptic contacts with colloidal gold-labeled vasopressin or neurophysin-II immunoreactive neurons were found in the paraventricular and supraoptic nuclei (PVN, SON) of the rat hypothalamus. Serotonin or GABA immunoreactive terminals were found to make synaptic contacts with oxytocin or neurophysin I immunoreactive neurons in the PVN and SON. These findings suggest that the secretion of vasopressin- or oxytocin-containing neurons may be regulated via synapses by catecholaminergic, GABAergic or serotonergic neurons. As to the innervation of hypothalamic hormone-containing neurons, catecholamine terminals showing selective uptake of ^3H-NA or 5-OHDA were found to make synaptic contacts with LHRH immunoreactive neurons in the medial preoptic area, with CRH immunoreactive heurons in the PVN and with GRH immunoreactive neurons in the arcuate nucleus of the rat hypothalamus. These provide morphological evidences for catecholaminergic control of the release of LHRH, CRH or GRH, at the hypothalamic level. Furthermore, colloidal gold-labeled TRH immunoreative terminals were found to make synaptic contact with PAP-lebeled GEH immunoreactive neurons in the arcuate nucleus. From this finding, TRH-containing neurons may be considered to innervate GRH secretion or to have some other functions via synapses in the hypothalamic arcuate nucleus of the rat.

为了在电镜水平上观察大鼠下丘脑神经分泌神经元的突触传入，采用了免疫细胞化学和放射自显影相结合的免疫细胞化学和免疫细胞化学双标记技术，并在同一组织切片上分别标记了~ 3 H-去甲肾上腺素（^~ 3 H-NA）和5-羟基多巴胺（5-OHDA）。在大鼠下丘脑室旁核（PVN）和视上核（SON）内，有过氧化物酶-抗过氧化物酶（PAP）标记的酪氨酸羟化酶、γ-氨基丁酸（GABA）或5-羟色胺（5-HT）免疫反应阳性终末与胶体金标记的加压素或神经垂体素-Ⅱ（neurophysin-II）免疫反应阳性神经元形成突触联系。发现5-羟色胺或GABA免疫反应终末与PVN和SON中的催产素或神经垂体素I免疫反应神经元形成突触接触。这些发现表明，分泌的加压素或催产素的神经元可能是通过突触调节的儿茶酚胺能，GABA能或多巴胺能神经元。在下丘脑含胆碱能神经元的神经支配中，选择性摄取^3 H-NA或5-OHDA的儿茶酚胺终末与视前内侧区的LHRH免疫反应阳性神经元、室旁核的CRH免疫反应阳性神经元和弓状核的GRH免疫反应阳性神经元形成突触联系。这些结果为下丘脑水平上LHRH、CRH或GRH的释放受儿茶酚胺能控制提供了形态学证据。此外，胶体金标记的TRH免疫反应终末与弓状核内PAP标记的GEH免疫反应神经元形成突触接触。由此推测，大鼠下丘脑弓状核内含TRH神经元可能通过突触支配GRH分泌或具有其他功能。