Nature and Physiological significance of clear microvesicles in the neuroendocrine nerve endings

神经内分泌神经末梢透明微泡的性质和生理意义

• 批准号: 05670026
• 负责人: NAKAI Yasumitsu
• 金额: $ 1.28万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670026/
• 关键词: hypothalamus; hypophysis; neurosecretory ending; microvesicles; glutamate; glutamate receptor; immunoelectron microscopy; 視床下部下垂体系; 神経分泌ニューロン; グルタミン酸レセプター; 後葉ホルモン; チロシン水酸化酵素

To clarify the nature and physiological significance of the clear microvesicles within the neurosecretory nerve endings of the rat hypothalamo-hypophysial system, ultrastructural localization of some neuroactive substances in the microvesicles has been examined by immunoelectron microscopy.1.Glutamate immunoreactivityUltrastructural distribution of glutamate immunoreactivity was observed using the post-embedding immunogold staining method. In the neurohypophysis, glutamate immunoreactivity was found to be localized in the area where numerous microvesicles aggregate Many immunogold particles were observed in the interior of microvesicles.2.Glutamate receptor immunoreactivityImmunoparticles for N-methyl-D-Aspartate (NMDA)-RI receptor was localized to the microvesicles within the nerve endings in the periventricular uncles of the hypothalamus.3.Tyrosine hydroxylase (TH) immunoreactivityTH-like immunoreactivity was found in the aggregate area of microvesicles within the catecholamine-containing nerve endings of the hypothalamus (A12, A14).4.Synaptophysin immunoreactivity was found in the microvesicles within the neurosecretory endings of the neurohypophysis. The above findings of ultrastructural localizations of glutamate and its receptor suggest that glutamate play some important roles of hormone secretion in the neurosecretory endings through autorine mechanism.

为了阐明大鼠下丘脑 - 寄生系统系统神经分泌神经端的性质和生理意义，通过免疫电脑的免疫剂量分布，通过免疫素质的分布来检查微腔中某些神经活性物质的超微结构定位，对微泡中的某些神经活性物质进行了超微结构定位。后填充免疫金染色方法。 In the neurohypophysis, glutamate immunoreactivity was found to be localized in the area where numerous microvesicles aggregate Many immunogold particles were observed in the interior of microvesicles.2.Glutamate receptor immunoreactivityImmunoparticles for N-methyl-D-Aspartate (NMDA)-RI receptor was localized to the microvesicles within the nerve endings in the下丘脑的周围室叔叔叔羟基羟化酶（Th）在微泡中的微泡中的骨料区域中发现了catecholamine catecholamine catecholamine catecholamine cantecholamus的神经终止的免疫反应性的免疫反应性（A12，A14）。神经息护物的结尾。上述谷氨酸超微结构局部及其受体的结果表明，谷氨酸通过自动机制在神经分泌末端中发挥了一些重要的激素分泌作用。

项目成果

落合英彦、中井康光: "包埋後金コロイド法によるニューロン内カテコールアミン合成酵素の免疫電顕的観察" 解剖誌. 68. 644 (1993)

Hidehiko Ochiai、Yasumitsu Nakai：“通过后包埋金胶体法对神经元内儿茶酚胺合酶进行免疫电子显微镜观察”解剖杂志 68. 644 (1993)。

Seiji Shioda: "Serotonergic innervation of vasopressin immunoreactive neurons in the rat hypothalamus as revealed by double labeling immunocytochemistry." The Showa University Journal of Medical Sciences. 5. 193-201 (1993)

Seiji Shioda：“通过双标记免疫细胞化学揭示了大鼠下丘脑中加压素免疫反应性神经元的血清素能神经支配。”

Nakai,Y.and Ochiai,H.: "Ultrastructural Aspects of Glutamate-like Immunoreactive Neurons in the Rat Hypothalamo-Hypophysial System." Electron Microscopy 3A Application in Biological Sciences. 3A. 649-650 (1994)

Nakai,Y. 和 Ochiai,H.：“大鼠下丘脑-垂体系统中谷氨酸样免疫反应性神经元的超微结构。”

Wang, Q.-P.and Nakai,Y.: "Enkephalinergic Innervation of GABAergic Neurons in the Dorsal Raphe Nucleus of the Rat." Brain Res.Bull.32. 315-320 (1993)

Wang, Q.-P. 和 Nakai,Y.：“大鼠中缝背核中 GABA 能神经元的脑啡肽神经支配。”

Iwase,M.,Homma,I.Shioda,S. and Nakai,Y.: "Histamine immunoreactive Neurons in the Brain Stem of the Rabbit." Brain Res.Bull.32. 267-272 (1993)

岩濑，M.，本间，I.盐田，S.