Cytochemical observations of Neuronal Influence on Vasopressin Synthesis and secretion

神经元对加压素合成和分泌影响的细胞化学观察

• 批准号: 02670019
• 负责人: NAKAI Yasumitsu
• 金额: $ 1.54万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670019/
• 关键词: noradrenergic innervation; vasopressin cells; double immunostaining; medullary A1-lesin; vasopressin-mRNA; in situ hybridization; paraventricular and suprapotic nuclei; rat hypothalamus; カテコ-ルアミン性神経支配; バソプレシン; 生成分泌; 神経性制御; 情報伝達物質; 免疫細胞化学

Synaptic inputs of noradrenaline- and brain natriuretic peptide-containing terminals to arginine-vasopressin producing cells(AVP cells)were observed, respectively, in the supraoptic and paraventricular nuclei(SON, PVN)of the rat hypothalamus using double immunostaining. In addition, axon terminals orginated from the neurons in the Al/Cl cell group in the medulla oblongata have been found to make synapses with AVP cells in the PVN and the SON using a double labeling technique combining WGA-HRP anterograde tracing with AVP immunocytochemistry at electron microscopic level.Using in situ hybridization and immunocytochemistry, we ekamined neuronal influence on vasopressin(AVP)-gene expression in rat hypothalamus after unilateral electrolytic lesion of the ventrolateral medulla, containing catecholaminergic neurons forming the Al cell group. Because Al neurons are throught to be the source of noradrenergic terminals upon the magnocellular neurons that release AVP dominantly.In the normal con … More trol rat, there was no difference in AVP-MRNA levels in magnocellular neurons between, the PVN and SON nuclei. However, unilateral Allesion caused AVP-MRNA levels between the normal and the lesioned rats. Furthermore, stimulation of water-deprivation in the lesioned rat increased in AVP-mRNA levels significantly not only in the SON but also in the PVN. I these experiments, there was no significant difference in rat plasma osmolarity and electrolytes(Na^+, K^+, Ca^<++>)(p<0.05).These results indicate that the AVP-gene expression in rat magnocellular neurons in differentially influenced between the PVN and SON by the neuronal regulation through the ventrolateral medulla, and at least in part, some pathways may through Al area to contribute to increase in levels in the PVN. Moreover, it is clarified that Al lesion does not inhibit the response of waterdeprivation in magnocellular neurons in both nuclei, suggesting deprovation in magnocellular neurosecretory neurons in both nuclei, suggesting different pathways and different mechanisms concerning to the AVP-gene expression. Less

用免疫双染法观察了大鼠下丘脑视上核和室旁核（SON，PVN）内含去甲肾上腺素和脑钠肽的终末对精氨酸加压素产生细胞（AVP细胞）的突触输入。此外，应用WGA-HRP顺行追踪和AVP免疫细胞化学双标记技术，在电镜水平上发现延髓内Al/Cl细胞群神经元的轴突终末与室旁核和视上核内的AVP细胞形成突触，应用原位杂交和免疫细胞化学技术，我们观察了电解损毁大鼠下丘脑腹外侧区（含儿茶酚胺能神经元，形成A1细胞群）后，下丘脑内神经元对加压素（AVP）基因表达的影响。由于A1神经元可能是主要释放AVP的大细胞神经元上的去甲肾上腺素能终末的来源，在正常条件下， ...更多信息 对照组大鼠，PVN和SON核大细胞神经元中AVP-MRNA水平无差异。而单侧Al 2+毁损后，AVP-mRNA水平则介于正常组和毁损组之间。此外，水剥夺刺激损伤大鼠不仅在SON而且在PVN中的AVP-mRNA水平显着增加。在这些实验中，大鼠血浆渗透压和电解质没有显著差异（Na^+，K^+，Ca^+<++>）（p<0.05）。这些结果表明，大鼠PVN和SON大细胞神经元中的AVP基因表达受到通过延髓腹外侧区的神经元调节的差异影响，并且至少部分地，一些途径可能通过Al区域来促进PVN中水平的增加。此外，铝损伤并不抑制两个核团的大细胞神经元对水剥夺的反应，这表明两个核团的大细胞神经分泌神经元的功能下降，表明AVP基因表达的不同途径和不同机制。少

项目成果

塩田 清二: "Electron microscopic studies of medullary synaptic inputs to vasopressinーcontaining neurons in the hypothalamic paraventricular nucleus." Journal of Electron Microscopy. 39. 501-507 (1990)

Seiji Shiota：“下丘脑室旁核髓质突触输入的电子显微镜研究”，《电子显微镜杂志》39. 501-507 (1990)。

Shioda,S: "Electron microscopic studies of medullary synaptic inputs to vasopressinーcontaining neurons in the hypothalamic paraventricular nucleus." Journal of Electron Microscopy. 39. 501-507 (1990)

Shioda, S：“下丘脑室旁核髓质突触输入的电子显微镜研究。” 电子显微镜杂志 39. 501-507 (1990)。

内藤 延子: "Differential neuronal influence on vasopressin mRNA levels in neurons of the paraventricular and the supraoptic nucleus after lesion of the ventrolateral medulla in rats." Neuroscience Letters.

Nobuko Naito：“大鼠腹外侧髓质损伤后，对室旁核和视上核神经元中加压素 mRNA 水平的差异神经影响。”

Shioda,S: "NPYーcatecholaminergic innervation of oxytocin neurons in the rat hypothalamus by triple immunolabeling electron microscopy." Proceedings of the Histochemical Society Journal of Histochemistry and Cytochemistry. 38. 1075 (1990)

Shioda, S：“通过三重免疫标记电子显微镜观察大鼠下丘脑催产素神经元的 NPY 儿茶酚胺神经支配。”《组织化学与细胞化学杂志》杂志 38. 1075 (1990)。

千田 隆夫: "Immunocytochemical localization of synaptophysin(protein p38)in nerve terminals of rat neurohypophysis." Archives of Histology and Cytology. 54. 233-240 (1991)

Takao Senda：“大鼠神经垂体神经末梢突触素（蛋白质 p38）的免疫细胞化学定位。”组织学和细胞学档案 54。233-240（1991）