Cytochemical observations of Neuronal Influence on Vasopressin Synthesis and secretion

神经元对加压素合成和分泌影响的细胞化学观察

• 批准号: 02670019
• 负责人: NAKAI Yasumitsu
• 金额: $ 1.54万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02670019/
• 关键词: noradrenergic innervation; vasopressin cells; double immunostaining; medullary A1-lesin; vasopressin-mRNA; in situ hybridization; paraventricular and suprapotic nuclei; rat hypothalamus; カテコ-ルアミン性神経支配; バソプレシン; 生成分泌; 神経性制御; 情報伝達物質; 免疫細胞化学

Synaptic inputs of noradrenaline- and brain natriuretic peptide-containing terminals to arginine-vasopressin producing cells(AVP cells)were observed, respectively, in the supraoptic and paraventricular nuclei(SON, PVN)of the rat hypothalamus using double immunostaining. In addition, axon terminals orginated from the neurons in the Al/Cl cell group in the medulla oblongata have been found to make synapses with AVP cells in the PVN and the SON using a double labeling technique combining WGA-HRP anterograde tracing with AVP immunocytochemistry at electron microscopic level.Using in situ hybridization and immunocytochemistry, we ekamined neuronal influence on vasopressin(AVP)-gene expression in rat hypothalamus after unilateral electrolytic lesion of the ventrolateral medulla, containing catecholaminergic neurons forming the Al cell group. Because Al neurons are throught to be the source of noradrenergic terminals upon the magnocellular neurons that release AVP dominantly.In the normal con … More trol rat, there was no difference in AVP-MRNA levels in magnocellular neurons between, the PVN and SON nuclei. However, unilateral Allesion caused AVP-MRNA levels between the normal and the lesioned rats. Furthermore, stimulation of water-deprivation in the lesioned rat increased in AVP-mRNA levels significantly not only in the SON but also in the PVN. I these experiments, there was no significant difference in rat plasma osmolarity and electrolytes(Na^+, K^+, Ca^<++>)(p<0.05).These results indicate that the AVP-gene expression in rat magnocellular neurons in differentially influenced between the PVN and SON by the neuronal regulation through the ventrolateral medulla, and at least in part, some pathways may through Al area to contribute to increase in levels in the PVN. Moreover, it is clarified that Al lesion does not inhibit the response of waterdeprivation in magnocellular neurons in both nuclei, suggesting deprovation in magnocellular neurosecretory neurons in both nuclei, suggesting different pathways and different mechanisms concerning to the AVP-gene expression. Less

双免疫染色法观察大鼠下丘脑视上核和室旁核（SON， PVN）中含去甲肾上腺素和脑利钠肽末端分别向精氨酸-加压素产生细胞（AVP细胞）的突触输入。此外，利用电镜下WGA-HRP顺行示踪和AVP免疫细胞化学双标记技术，发现延髓Al/Cl细胞组神经元的轴突终末与PVN和SON中的AVP细胞形成突触。采用原位杂交和免疫细胞化学方法，研究了大鼠下丘脑腹外侧髓质单侧电损伤后神经元对抗利尿激素（AVP）基因表达的影响，其中儿茶酚胺能神经元组成Al细胞组。因为Al神经元被认为是主要释放AVP的大细胞神经元上的去甲肾上腺素能末端的来源。在正常对照大鼠中，PVN和SON核中大细胞神经元AVP-MRNA水平无明显差异。然而，单侧损伤引起正常大鼠和损伤大鼠之间AVP-MRNA水平的变化。此外，在损伤大鼠中，水剥夺刺激不仅显著增加了SON中的AVP-mRNA水平，而且显著增加了PVN中的AVP-mRNA水平。在这些实验中，大鼠血浆渗透压和电解质（Na^+、K^+、Ca^<++>）无显著差异（p<0.05）。这些结果表明，avp基因在PVN和SON大细胞神经元中的表达受到神经元通过腹外侧髓质的调节的差异影响，至少在一定程度上，某些通路可能通过Al区促进PVN水平的升高。此外，Al损伤不抑制双核大细胞神经元对缺水的反应，提示双核大细胞神经分泌神经元退化，提示avp基因表达的途径和机制不同。少

项目成果

塩田 清二: "Electron microscopic studies of medullary synaptic inputs to vasopressinーcontaining neurons in the hypothalamic paraventricular nucleus." Journal of Electron Microscopy. 39. 501-507 (1990)

Seiji Shiota：“下丘脑室旁核髓质突触输入的电子显微镜研究”，《电子显微镜杂志》39. 501-507 (1990)。

Shioda,S: "Electron microscopic studies of medullary synaptic inputs to vasopressinーcontaining neurons in the hypothalamic paraventricular nucleus." Journal of Electron Microscopy. 39. 501-507 (1990)

Shioda, S：“下丘脑室旁核髓质突触输入的电子显微镜研究。” 电子显微镜杂志 39. 501-507 (1990)。

内藤 延子: "Differential neuronal influence on vasopressin mRNA levels in neurons of the paraventricular and the supraoptic nucleus after lesion of the ventrolateral medulla in rats." Neuroscience Letters.

Nobuko Naito：“大鼠腹外侧髓质损伤后，对室旁核和视上核神经元中加压素 mRNA 水平的差异神经影响。”

Shioda,S: "NPYーcatecholaminergic innervation of oxytocin neurons in the rat hypothalamus by triple immunolabeling electron microscopy." Proceedings of the Histochemical Society Journal of Histochemistry and Cytochemistry. 38. 1075 (1990)

Shioda, S：“通过三重免疫标记电子显微镜观察大鼠下丘脑催产素神经元的 NPY 儿茶酚胺神经支配。”《组织化学与细胞化学杂志》杂志 38. 1075 (1990)。

千田 隆夫: "Immunocytochemical localization of synaptophysin(protein p38)in nerve terminals of rat neurohypophysis." Archives of Histology and Cytology. 54. 233-240 (1991)

Takao Senda：“大鼠神经垂体神经末梢突触素（蛋白质 p38）的免疫细胞化学定位。”组织学和细胞学档案 54。233-240（1991）