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Physiological significance of enzyme-activating substances in the neuroendocrine cells.

神经内分泌细胞中酶激活物质的生理意义。

基本信息

批准号：
07670036
负责人：
NAKAI Yasumitsu
金额：
$ 1.47万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670036/
关键词：
Hypothalamus Neurosecretory cells PACAP neurons Glutamate neurons NOS neurons Receptors Synapses Immunoelectron microscopy 神経分泌ニューロン PACAP PACAPレセプター nitric oxide synthase グルタミン酸グルタミン酸レセプター免疫電顕

项目摘要

Ultrastructural localization of pituitary adenylate cyclase activating polypeptide (PACAP), nitric oxide synthase (NOS) and glutamate in the neuroendocrine cells of the rat hypothalamus were observed to examine their functional significances by electron microscopic immunohistochemistry.PACAP-like immunoreactive axon terminals were found to make synapses with the vasopressin-like immunoreactive cell processes and cell bodies in the supraoptic nucleus of the rat hypothalamus using preembedding double immunostaining.In situ hybidization revealed high-level expression of PACAP type 1 receptor (PACAP R-1) mRNA in the vasopressin-like immunoreactive neurons in the supraoptic nucleus of the rat hypothalamus.We found the vasopressin-containing neurons in the supraoptic nucleus strongly expressed PACAP R-1 mRNA by combining immunohistichemical staining and in situ hybridization of the same tissue sections. These findings suggest that PACAP-containing neurons might regulate the secretion of vasopressin via PACAP type 1 receptor.PACAP may have a physiological role in the release of vasopressin, as well as in induction of the expression of the gene for vasopressin via regulation of the cytosolic level of cAMP.Concerning NOS-containing neurons in the dorsal raphe nucleus, the cytoarchitecture of the NOS-like immunoreactive neurons was similar to that of the serotonergic neurons. NOS-like immunoreactive axon terminals were found to make synapses with some unknown neurons.In the rat hypothalmic periventricular nucleus, glutamatergic and GABAergic axon terminals formed synaptic contacts on the glutamate receptor subunit (NMDA-R1) immunoreactive endrites. These results suggest that NMDA-R1 containing neurons could be regulated by excitatory and inhibitory neurons.

观察大鼠下丘脑神经内分泌细胞中垂体腺苷酸环化酶激活多肽（PACAP）、一氧化氮合酶（NOS）和谷氨酸的超微结构定位，并通过电子显微镜免疫组织化学检查其功能意义。采用预包埋双重免疫染色法对大鼠下丘脑视上核中的加压素样免疫反应性细胞突起和细胞体进行了研究。原位杂交显示大鼠下丘脑视上核中的加压素样免疫反应性神经元中 PACAP 1 型受体 (PACAP R-1) mRNA 高水平表达。通过结合免疫组织化学染色和相同组织切片的原位杂交，视上核中含有加压素的神经元强烈表达 PACAP R-1 mRNA。这些发现表明，含有 PACAP 的神经元可能通过 PACAP 1 型受体调节加压素的分泌。PACAP 可能在加压素的释放中具有生理作用，以及通过调节 cAMP 的胞质水平诱导加压素基因的表达。关于中缝背核中含有 NOS 的神经元， NOS 样免疫反应神经元与血清素能神经元相似。类似NOS的免疫反应性轴突末端被发现与一些未知神经元形成突触。在大鼠下丘脑室周核中，谷氨酸能和GABA能轴突末端在谷氨酸受体亚基（NMDA-R1）免疫反应性树突上形成突触接触。这些结果表明含有 NMDA-R1 的神经元可以受到兴奋性和抑制性神经元的调节。