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Plant mass propagation by an aero-liquid nutrient supply method

通过气液养分供给法进行植物大量繁殖

基本信息

批准号：
62460211
负责人：
NAGANO Toshihide
金额：
$ 4.48万
依托单位：
Tokyo University of Agriculture
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Lots of interest have been devoted to the environmental control of plant mass propagation in recent years. Conventional methods of plant tissue culture have been re-examined if they were really optimal in relation to the microenvironment around the plantlets. Agar media and test tubes have been widely used in plant tissue culture. They proved, however, to have problems in nutrient absorption and gas exchange (CO_2 and O_2) by the plantlets. By the liquid medium method, nutrient absorption was highly improved but the gas exchange still remained one of the limiting factors of the plantlet growth.To overcome these difficulties, a new method was proposed and tested in this study. by the new method nutrient was supplied as mists so that the plantlets will be well exposed not only to the nutrient containing media but also to the air. Two systems were contructed. In the first one, mists were produced using nozzles and supplied to the plantlets. In experiments using potato plantlets, the average growth rate was higher than that of the agar media. Uniformity of the growth was, however, poor due to problems in supporting the plantlets. Also the sterility of the system was insufficient. In the second system, an ultrasonic mist generator was used. Dry matter production was higher using this system than the conventional method. Reliability of the mist generator and the sterility remained to be improved.

近年来，植物大规模繁殖的环境控制研究受到了广泛的关注。传统的植物组织培养方法已经被重新审视，如果它们真的是最佳的小植株周围的微环境。琼脂培养基和试管在植物组织培养中得到了广泛的应用。但是，它们在养分吸收和气体交换（CO_2和O_2）方面存在问题。采用液体培养基培养法，虽然提高了植株对养分的吸收，但气体交换仍是影响植株生长的主要因素之一，为此提出了一种新的培养方法。通过新方法，营养物以雾状供应，使得小植株不仅充分暴露于含营养物的培养基，而且也充分暴露于空气。建立了两个系统。在第一个中，使用喷嘴产生雾并供应给幼苗。在马铃薯试管苗试验中，平均生长速率高于琼脂培养基。然而，由于支撑小植株的问题，生长的均匀性差。系统的无菌性也不足。在第二个系统中，使用超声波雾发生器。干物质生产高于使用该系统比传统的方法。雾发生器的可靠性和无菌性仍有待提高。