Plant mass propagation by an aero-liquid nutrient supply method

近年来，植物大量繁殖的环境控制引起了人们的极大兴趣。传统的植物组织培养方法已经被重新审查，看看它们是否真的是与植株周围的微环境有关的最佳方法。琼脂培养基和试管已广泛应用于植物组织培养。然而，他们证明，在养分吸收和气体交换(CO_2和O_2)方面存在问题。液体培养法虽然提高了植株对营养物质的吸收，但气体交换仍然是限制其生长的因素之一，为克服这些困难，本研究提出并验证了一种新的培养方法。通过这种新的方法，养分以雾的形式提供，使试管苗不仅能很好地暴露在含有营养的基质中，而且还能暴露在空气中。构建了两个系统。在第一种方法中，雾气是用喷嘴产生的，并提供给苗木。马铃薯试管苗的平均生长率高于琼脂培养基组分。然而，由于支持试管苗的问题，生长的一致性很差。此外，该系统的无菌性也不足。在第二个系统中，使用了超声波雾化发生器。与常规方法相比，该系统的干物质产量更高。雾化发生器的可靠性和无菌性能有待提高。

Lots of interest have been devoted to the environmental control of plant mass propagation in recent years. Conventional methods of plant tissue culture have been re-examined if they were really optimal in relation to the microenvironment around the plantlets. Agar media and test tubes have been widely used in plant tissue culture. They proved, however, to have problems in nutrient absorption and gas exchange (CO_2 and O_2) by the plantlets. By the liquid medium method, nutrient absorption was highly improved but the gas exchange still remained one of the limiting factors of the plantlet growth.To overcome these difficulties, a new method was proposed and tested in this study. by the new method nutrient was supplied as mists so that the plantlets will be well exposed not only to the nutrient containing media but also to the air. Two systems were contructed. In the first one, mists were produced using nozzles and supplied to the plantlets. In experiments using potato plantlets, the average growth rate was higher than that of the agar media. Uniformity of the growth was, however, poor due to problems in supporting the plantlets. Also the sterility of the system was insufficient. In the second system, an ultrasonic mist generator was used. Dry matter production was higher using this system than the conventional method. Reliability of the mist generator and the sterility remained to be improved.