Plant mass propagation by an aero-liquid nutrient supply method

近年来，植物群体繁殖的环境控制受到了广泛关注。传统的植物组织培养方法已经被重新审视，如果它们真的是最优的关系到周围的微环境的植物。琼脂培养基和试管在植物组织培养中得到了广泛应用。然而，事实证明，这些植物在营养吸收和气体交换（CO_2和O_2）方面存在问题。液体培养基法提高了植株的养分吸收，但气体交换仍是制约植株生长的因素之一。为了克服这些困难，本研究提出了一种新的方法并进行了试验。通过这种新方法，营养物以雾霭的形式供给，使幼苗既能很好地暴露于营养物培养基中，又能很好地暴露于空气中。构建了两个系统。在第一种方法中，使用喷嘴产生雾气并供应给植株。马铃薯苗的平均生长率高于琼脂培养基。然而，由于幼苗的扶植问题，生长的均匀性较差。此外，该系统的无菌性不足。在第二个系统中，使用了超声波雾发生器。该系统的干物质产量高于常规方法。雾发生器的可靠性和无菌性有待提高。

Lots of interest have been devoted to the environmental control of plant mass propagation in recent years. Conventional methods of plant tissue culture have been re-examined if they were really optimal in relation to the microenvironment around the plantlets. Agar media and test tubes have been widely used in plant tissue culture. They proved, however, to have problems in nutrient absorption and gas exchange (CO_2 and O_2) by the plantlets. By the liquid medium method, nutrient absorption was highly improved but the gas exchange still remained one of the limiting factors of the plantlet growth.To overcome these difficulties, a new method was proposed and tested in this study. by the new method nutrient was supplied as mists so that the plantlets will be well exposed not only to the nutrient containing media but also to the air. Two systems were contructed. In the first one, mists were produced using nozzles and supplied to the plantlets. In experiments using potato plantlets, the average growth rate was higher than that of the agar media. Uniformity of the growth was, however, poor due to problems in supporting the plantlets. Also the sterility of the system was insufficient. In the second system, an ultrasonic mist generator was used. Dry matter production was higher using this system than the conventional method. Reliability of the mist generator and the sterility remained to be improved.