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Studies on the antitumor activity of seaweed and phytoplankton

海藻和浮游植物的抗肿瘤活性研究

基本信息

批准号：
62470129
负责人：
NODA Hiroyuki
金额：
$ 3.84万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1989
项目状态：
已结题

项目摘要

1. Antitumor activity of seaweed and phytoplankton.(1). After the preoral administration of powdered 57 species of seaweed and phyioplanlcton to mice inoculated wit)i Ehrlich car cinoma, Scytosiplion lomentaria showed the highest inhibition rate (69.8%), followed by Lessonia nigrescens (60.0%), Laminaria japonica (57.6%), Porphyra Xezoensis (53.2%) and Euchetima ,&,elatinae (52.1%).(2.). After the intraperitoneal administration to mice inoculated with Meth A fibrosarcoma, the inhibition rate were highest in Sargasstim patens (55.5%), followed by Etichetima cottonoii (39.8%), S. ringgoldianum (39.1%), L. atigiistats (36.6%) and Ecklonia cai,a (35.9%).2. Investigation of antitumor substances in seaweed and phytoplankton by cytotoxicity in human cell cultures.Seventy seven samples of polysaccharides, water-soluble high iixolecxilar weight fraction, water-soluble low molecular weight fraction and tannin-like substances were extracted from fifty seven species of seaweed and phytoplankton. Antitumor effect of these samples were investigated by growth inhibition rate against human tumor cell WI-38 VA-13.(1). Polysaccharides. Green-algal heteropolysaccharide from Monostroma nitidtim showed strong activity of IC_<50>=18.6 mug/ml.(2).Water-soluble high molecular weight fraction. Inhibition of tumor growth was found for Hijikia ftisiforme(IC_<50>=41.6 mug/ml).(3).Water-soluble low molecular weight fraction. Of the eleven effective species, Hetezrochordaria abietina were found to show highest inhibition rate (IC_<50>=2.7 mug/ml).(4).Tannin-like substances. Ascophyllum nodostim showed high inhibition rate (IC_<50>=10.1 mug/Ml).

1.海藻和浮游植物的抗肿瘤活性。(1)小鼠口服57种海藻和浮游藻粉后，对艾氏癌细胞的抑制率最高(69.8%)，其次是黑藻(60.0%)、海带(57.6%)、条斑紫菜(53.2%)和海带(52.1%)。对接种Meth A纤维肉瘤的小鼠，以马尾藻的抑制率最高(55.5%)，其次是棉环马尾藻(39.8%)、马尾藻(39.1%)、马尾藻(36.6%)和马尾藻(35.9%)。从57种海藻和浮游植物中提取了77种多糖、水溶性高分子量组分、水溶性低分子量组分和单宁类物质。通过对人肿瘤细胞株WI-38、VA-13的生长抑制率考察样品的抗肿瘤作用。多糖类。两面针绿藻多糖具有较强的活性，IC_(50)=18.6µg/ml。(2)水溶性高分子量。对肿瘤生长有抑制作用(IC_(50)=41.6µg/ml)。在11种有效成分中，以山毛菜的抑制率最高(IC_(50)=2.7µg/ml)。(4)单宁类物质。结瘤假单胞菌表现出较高的抑制率(IC_(50)=10.1µg/ml)。