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Neuronal network analysis of functional states in the cerebral cortex

大脑皮层功能状态的神经元网络分析

基本信息

批准号：
62480110
负责人：
OSHIMA Tomokazu
金额：
$ 3.9万
依托单位：
Tokyo Metropolitan Institute for Neurosciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

As a part of researches to elucidate a variety of functional atates of the cerebral cortex as the integrative actions of neuronal assenblies, we have analysed the neuronal mechanisms of the electrocortical spindle wave. The encephale isole cat preparations were maintained atraumatically in the sound-attenuated and electrically shielded room. The electrocorticogram was monitored on the precruciate motor cortex. The intracellular potentials of all the layer neurones were recorded nearby through the glass capillary microelectrode manipulated by a remote control from outside the room. The results were as follows: 1) The laminae V-VI neurones exhibited two types of rhythmic depolarizing potentials (RDPs), RDP-I and RDP-II, respectively in phase of type I positive and type II negative spindle waves. Their amplitudes were positively correlated in each type throughout the characteristically developing and decaying processes of the spindle. 2) These RDPs were proved to be the excitatory postsynaptic potentials (EPSPs) of dendritic origin by their amplitude changes dependent susceptively on the spontaneous membrane potential fluctuation, but contrastively little dependent on the membrane potential altered by intracellular step current injections. These dendritic characteristics were confirmed for comparison in the monosynaptic EPSPs of the same sample neurones produced by epicortical or thalamic stimulation. 3) RDPs also occurred in laminae I-III neurones as EPSPs, but with smaller amplitude and less dendritic nature than laminae V-VI neurones. These results have indicated that rhythmic components of the spindle are generated mainly by dendritic EPSPs in the deep layer neurones on which at least two different subcortical afferent inputs impinge at different depths from the cortical surface.

作为阐明大脑皮层作为神经元突触整合作用的各种功能状态的研究的一部分，我们分析了皮层电梭波的神经机制。将脑隔离猫制剂无创伤地保存在声音衰减和电屏蔽室中。在交叉前运动皮层上监测皮层电图。用玻璃毛细管微电极在室外遥控器操作，记录近距离各层神经元的细胞内电位。结果如下：（1）V-VI层神经元在I型纺锤波正相和II型纺锤波负相时相分别出现两种类型的节律性去极化电位（RDP-I和RDP-II）。它们的振幅在每种类型的纺锤体的整个特征性的发展和衰减过程中呈正相关。2)这些RDPs的幅度变化依赖于自发的膜电位波动，而不依赖于细胞内阶跃电流注入引起的膜电位变化，证明它们是树突状起源的兴奋性突触后电位（EPSP）。这些树突状特征被证实比较在单突触EPSP的相同样品神经元产生的皮层或丘脑刺激。3)RDPs也发生在板层I-III神经元的EPSP，但较小的振幅和较少的树突状的性质比板层V-VI神经元。这些结果表明，节律性成分的纺锤体产生的主要树突EPSPs在深层神经元上，至少有两个不同的皮层下传入输入撞击在不同的深度从皮层表面。