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Pathophysiological investigation of respiratory failure;Changes in energy metabolism using ^<31>P-NMR method

呼吸衰竭的病理生理学研究；利用^<31>P-NMR法测定能量代谢的变化

基本信息

批准号：
62480206
负责人：
YAMABAYASHI Hajime
金额：
$ 3.71万
依托单位：
Tokai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

1. To investigate the cellular acclimatization of energy metabolism to chronic hypoxia, concentration of high energy phosphate compounds of cultivated alveolar and peritoneal macrophages were measured by Luciferin-Luciferase methods. ATP contained 1mg of protein in alveolar and peritoneal macrophages was not significantly different. Alveolar macrophages were cultivated for 48 hr. under aerobic and anaerobic conditions. ATP in anaerobically cultivated macrophage is decreased by 20% compared with aerobically cultivated one. Creatine phosphate (CP) and energy charge were not significantly different. These results suggest that production of ATP is decreased in anaerobically cultivated macrophages due to inhibition of oxidative phosphorylation.2. Wister rats were exposed to normobaric hypoxic condition for 6 W. High energy phosphate compounds of upper thigh muscle in control and hypoxic rats were measured by Luciferin-Luciferase methods and ^<31>P-NMR spectroscopy. There is no difference of high energy phosphate compounds between control and hypoxic rats in both methods.3. Normal subject and patients with chronic respiratory failure were studied for the changes of high energy phosphate compounds during exercise. These subjects placed their arm into the magnet of the NMR spectroscopy and were asked to contract isotonically for 3 min. ^<31>P-NMR spectrum were taken every 3 min at rest, during exercise and recovery. During exercise, peak of inorganic phosphate increased and deviated to CP peak, and the CP peak decreased. In patients with chronic respiratory failure, intracellular PH decreased more remarkably than normal subject, CP/Pi during exercise was lower than that in normal subject. These results indicate that anaerobic metabolism in the patients may be activated more during exercise than in normal subject.

1.为探讨细胞能量代谢对慢性缺氧的适应性，采用荧光素酶法测定了培养的肺泡巨噬细胞和腹腔巨噬细胞高能磷酸化合物的含量。肺泡巨噬细胞和腹腔巨噬细胞中ATP含量均为1 mg蛋白，差异无显著性。在需氧和厌氧条件下培养肺泡巨噬细胞48小时。与有氧培养的巨噬细胞相比，厌氧培养的巨噬细胞的ATP减少了20%。磷酸肌酸（CP）和能荷无显着差异。这些结果表明，缺氧培养的巨噬细胞由于氧化磷酸化的抑制，ATP的产生减少. Wister大鼠暴露于常压低氧环境6 W。用荧光素酶法和~ 1H-NMR法测定了正常和低氧大鼠大腿肌肉高能磷酸化合物<31>的含量。在两种方法中，对照组和低氧组大鼠的高能磷酸盐化合物含量无差异.本文研究了正常人和慢性呼吸衰竭患者运动时高能磷酸化合物的变化。这些受试者将他们的手臂放入NMR光谱仪的磁体中，并被要求等张收缩3分钟。<31>在休息、运动和恢复期间，每3分钟采集一次13 P-NMR光谱。运动过程中无机磷峰值升高并向CP峰偏移，CP峰降低。慢性呼吸衰竭患者细胞内PH值较正常人明显降低，运动时CP/Pi较正常人低。提示运动时患者的无氧代谢可能比正常人更活跃。