Treatment of lung metastasis from renal cell carcinoma with lymphokine-activated killer cells and interleukin-2.

首先研究了诱导淋巴因子激活的杀伤细胞(LAK)的条件。1%、2%和5%的血清浓度对LAK活性无明显影响。培养瓶中外周血淋巴细胞的最佳浓度为2×106/ml，重组白介素2产生LAK细胞的最有效浓度为3U/ml，对Daudi细胞的杀伤作用在培养第2天达到高峰。1例肾细胞癌肺转移患者用LAK细胞和D2IL-2联合治疗。在14个月中，LAK细胞和D2R@&GT；D2IL-2共11次，前2次静脉注射，9次经支气管动脉注射。注射LAK细胞总数为16.1×10~(-1)×10~(-1)~(-1)细胞。诱导的LAK活性逐渐降低，在第6次处理时达到最低值。从第6次治疗开始，在注射LAK细胞的前一天进行血浆置换。诱导的LAK活性在第7次处理后恢复。尽管进行了这些治疗，但转移灶的大小逐渐增大。

The conditions to induce lymphokine-activated killer (LAK) cells were investigated at first. Concentrations of serum in culture media, 1, 2 and 5% had no effect on induced LAK activity. The best density of peripheral blood lymphocytes in culture bottle was 2x10^6/ml, and the most effective concentration of recombinant interleukin-2 (<@2r<@D2IL-2) to generate LAK cells was 3u/ml. Cytotoxicity against Daudi cell appeared on the second day of culture and reached a peak on the third day. There was no difference in induced LAK activity between standing and rolling culture.One patient with lung metastasis from renal cell carcinoma was treated with LAK cells and <@2r<@D2IL-2. LAK cells and <@D2r@>D2IL-2 were administered 11 times during 14 months, the first 2 times intravenously and 9 times via bronchial artery. The total number of LAK cells injected was 16.1x10<@D19@>D1 cells. Induced LAK activities gradually decreased and reached minimum at 6th treatment. From the 6th treatment plasma exchange was performed the day before administration of LAK cells. Induced LAK activities recovered from the 7th treatment. Despite these treatments the size of the metastatic lesion gradually increased.