Structure, Function, and Diseases of Fibronectin and Vitronectin

纤连蛋白和玻连蛋白的结构、功能和疾病

• 批准号: 62490007
• 负责人: HAYASHI Masao
• 金额: $ 3.52万
• 依托单位: Ochanomizu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62490007/
• 关键词: Vitronectin; Glycoprotein; Blood; Collagen; Monoclonal antibody; Purification; Cell spreading; ドメイン; 細胞接着因子; 細胞伸展因子; 糖タクパク質; 血奨; 血清

Vitronectin discovered in 1983 is a new plasma glycoprotein, which has been shown to have biological activities concerning cell spreading, blood coagulation, and complement action.We have investigated the structure and function of vitronectin and the results we have obtained are as follows.1. We have developed the method to measure vitronectin concentration in human blood immunologically. (1) The concentration of vitronectin in human plasma was remarkably low in the person having liver failures. (2) Human blood samples could be classified into three distinct vitronectin types depending on the ratios of 65 kDa and 75 kDa polypeptides of vitronectin. (3) The vitronection blood type was not related with any deseases tested so far. 2. We have succeeded in purification of vitronectin from plasma or sera of bovine, horse, chicken, porcine, and rabbit with the same new procedure developed for human plasma very recently by us. 3. We have cloned 4 mouse hybridoma producing anti-human vitronection monoclomal antibodies, M1, M2, M4, and M5. Using these monoclonal antibodies, collagen-binding domain of vitronectin was suggested to exist on the N-terminal half of vitronectin molecule. 4. We have purified a few vitronectin fragments cleaved with formic acid. One of these fragments seemed to correspond to the collagen-binding domain.

玻连蛋白是1983年发现的一种新的血浆糖蛋白，已被证明具有细胞铺展、凝血和补体作用等生物活性。我们对玻连蛋白的结构和功能进行了研究，得到的结果如下： 1．我们开发了免疫学测量人体血液中玻连蛋白浓度的方法。 (1)肝功能衰竭患者血浆中玻连蛋白的浓度显着降低。 (2) 根据玻连蛋白 65 kDa 和 75 kDa 多肽的比例，人类血液样本可分为三种不同的玻连蛋白类型。 (3)目前为止，玻璃连接血型与所检测的任何疾病均无相关性。 2. 我们已经成功地从牛、马、鸡、猪和兔的血浆或血清中纯化了玻连蛋白，其方法与我们最近为人血浆开发的相同新程序相同。 3.我们克隆了4个产生抗人体外连接单克隆抗体的小鼠杂交瘤，M1、M2、M4和M5。使用这些单克隆抗体，表明玻连蛋白的胶原蛋白结合结构域存在于玻连蛋白分子的 N 末端部分。 4. 我们纯化了一些用甲酸切割的玻连蛋白片段。这些片段之一似乎对应于胶原蛋白结合域。

项目成果

Kubota,K.: Cell Struct.Funct.

Kubota,K.：细胞结构.功能。

T.Yatohgo: Cell Struct.Funct.13. 281-292 (1988)

T.Yatohgo：细胞结构.功能.13。

Yatohgo,T.: Cell Struct.Funct.

Yatohgo,T.：细胞结构.功能。

K. Kubota: "Three types of vitronectin in human blood." Cell Struct. Funct.13. 123-128 (1988)

K. Kubota：“人类血液中的三种类型的玻连蛋白。”

T. Yatohgo: "Novel purification of vitronectin from human plasma by heparin affinity chromatography." Cell Struct. Funct.13. 281-292 (1988)

T. Yatohgo：“通过肝素亲和层析从人血浆中纯化玻连蛋白的新方法。”