Gene Expression of Cell-adhesive Molecule Vitronectin

细胞粘附分子玻连蛋白的基因表达

• 批准号: 09490012
• 负责人: HAYASHI Masao
• 金额: $ 2.24万
• 依托单位: Ochanomizu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09490012/
• 关键词: integrin αv; vitronectin; cell adhesion; transcriptinal regulation; promoter; transcription factor; Ets; Sp1; 胚性腫瘍細胞株; ヒトロネクチン; 核タンパク質; シスエレメント; 細胞外マトリックス

1. Identification of cis-acting elements of integrin α v in mouse melanoma cells. (Head Investigator, Prof. Masao Hayashi)We have cloned mouse integrin promoter region from mouse genome library and determined the nucleotide sequence of 1.8 kbp region upstream of the transcription initiation site (+1 bp). We have revealed the three transcriptional regulatory elements between -108 and +1 bp. One element from -27 to -16 bp binds to Sp1, and the two other elements from -61 to -54 bp and from -106 to -98 bp bind to Ets family. The element from -61 to -54 bp binds to Ets-1, one of the Ets family. Mutations or deletions in any one of the 3 cis-acting elements remarkably decreased the promoter activity. These results indicate that Sp1 and Ets cooperate to activate the 109-bp α v promoter region.2. Characterization of vitronectin promoter region (Investigator, Dr. Yasunori Miyamoto)We showed that a region from -19 to +54 bp is essential for the initiation of transcription in mouse vitronectin gene. This region does not have TATA box. It forms a complex with nuclear proteins extracted from human hepatoma HepG2 cells. We determined the nucleotide sequence between -1.8 kbp and -3.7 kbp in 5'-flanking region of vitronectin gene.

1.小鼠黑色素瘤细胞中整合素αv顺式作用元件的鉴定。 （首席研究员，Masao Hayashi教授）我们从小鼠基因组文库中克隆了小鼠整合素启动子区域，并确定了转录起始位点上游1.8 kbp区域（+1 bp）的核苷酸序列。我们揭示了-108 和+1 bp 之间的三个转录调控元件。 -27 至 -16 bp 的一个元件与 Sp1 结合，-61 至 -54 bp 和 -106 至 -98 bp 的另外两个元件与 Ets 家族结合。 -61 至 -54 bp 的元素与 Ets 家族之一的 Ets-1 结合。 3个顺式作用元件中任何一个的突变或缺失都会显着降低启动子活性。这些结果表明Sp1和Ets协同激活109bp的αv启动子区。2．玻连蛋白启动子区域的表征（研究者，Yasunori Miyamoto 博士）我们表明，-19 至 +54 bp 的区域对于小鼠玻连蛋白基因的转录起始至关重要。该地区没有TATA盒子。它与从人肝癌 HepG2 细胞中提取的核蛋白形成复合物。我们确定了玻连蛋白基因5'侧翼区-1.8 kbp 和-3.7 kbp 之间的核苷酸序列。

项目成果

季周洋: "A protein reacted with anti-vitronectin antibody accumulates in tumors derived from B16F10 melanoma cells."Cell Struct.Funct.. 23. 193-199 (1998)

Hiroshi Kishu：“与抗玻连蛋白抗体反应的蛋白质在 B16F10 黑色素瘤细胞衍生的肿瘤中积聚。”Cell Struct.Funct.. 23. 193-199 (1998)

門脇 寿枝: "RGD peptide inhibits anchorage-independent growth of myeloid leukemia M1 cells."Cell Struct.Funct.. 24. 570 (1999)

Toshie Kadowaki：“RGD 肽抑制骨髓性白血病 M1 细胞的锚定非依赖性生长。”Cell Struct.Funct.. 24. 570 (1999)

宮本 泰則: "Integrin αv promoter activity is regulated by transcription factors Sp1 and Ets."Cell Struct.Funct.. 24. 570 (1999)

Yasunori Miyamoto：“整合素 αv 启动子活性受转录因子 Sp1 和 Ets 调节。”Cell Struct.Funct.. 24. 570 (1999)

田嶌 亜紀子: "Mouse integrin αv promoter is regulated by transcription factors Ets and Sp1 in melanoma cells."Biochim.Biophys.Acta. (印刷中). (2000)

Akiko Tashima：“小鼠整合素 αv 启动子受黑色素瘤细胞中转录因子 Ets 和 Sp1 的调节。”Biochim.Biophys.Acta（印刷中）。

Miyamoto, Y., Suzuki, Y., Hayashi, M.: "Trans-acting factors their binding sites on TATA-less mouse vitronectin promoter"Mol. Biol. Cell. 9. 426a (1998)

Miyamoto, Y.、Suzuki, Y.、Hayashi, M.：“反式作用因子在无 TATA 的小鼠玻连蛋白启动子上的结合位点”Mol。