权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Role of pineal, retina and adrenal for formation of circadian rhythms of physiological function of chickens and cattle.

松果体、视网膜和肾上腺对鸡和牛生理功能昼夜节律形成的作用。

基本信息

批准号：
63480079
负责人：
TANABE Yuichi
金额：
$ 4.29万
依托单位：
Faculty of Agriculture, Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

项目摘要

The levels of melatonin in pineal gland and retina show a circadian rhythm in phase with a light-dark cycle.; levels of melatonin increase notably at night and, inversely, are low in the daytime.At 0 day of age, White Leghorn chicks were either pinealectomized (PX), sham-pinealectomized (SP), blinded by complete bilateral orbital enucleation (BL), pinealectomized and blinded (PX-BL), or left intact (I). All birds were held in a chick brooder with a common 12L:12D light/dark cycle, lights on from 06:00 to 18:00. Lighting was provided by ceiling 40-W fluorescent bulbs, with an average intensity of 200-300 lux at the chick blooder. Blood sample were obtained from l-, 7-, 16-, 29-, 65- and 100-day-old cockrels at 8, 12, 16, 20, 24, 4 and 8 o'clock each day. Melatonin concentrations in the plasma were determined by a radioimmunoassay. The plasma melatonin,concentrations of control (I and SP) birds showed a prominent daily rhythm with a peak at mid scotophase. A small plasma melatonin rhythm … More (about 30% of the control levels at mid-scotophase) was detected in the PX birds at each day of age. In BL bij:-ds, plasma melatonin showed a small daily rhythm ( 30% of the control levels at mid-scotophase ) on day 1. Although the melatonin level at mid- scotophase increased with advancing age, the level was same as the control (I and SP) after 16 days. PX-BL birds showed little daily rhythm.White Leghorn chicks were.incubated under DD. After hatching some were kept under DD in continuous White noise to counteract the influence of noise and the other were kept under DD with 12h (6:00-18:00) White noise. Pineal glands and blood samples were obtained from l-, 7-, 13- and 24-day-old chickens at 8, 12, 16, 20, 24, 4 and 8 o'clock of each day. melatonin contents in pineal glands and melatonin concentrations in the plasma were determined by a radioimmunbassay. Both melatonin content of pineal glands and melatonin concentrations in plasma did not, however, peak under DD and continuous white noise conditions at any time during the experiment. In the birds kept under DD in 12h White noise, melatonin content of pineal glands peaked at the multime during 6:00-18:00 at each day of age. So this result indicated the signal of noise was a potential Zeitgeber for forming the rhythm. Less

松果体和视网膜中褪黑激素的水平表现出昼夜节律，与光-暗周期相一致。褪黑激素的水平在夜间显著增加，相反，在白天则较低。0日龄时，分别切除松果体（PX）、假松果体（SP）、双侧眼眶完全去核（BL）、切除松果体并盲化（PX-BL）或完整保留(I)。所有的雏鸟都饲养在一个育雏笼中，有一个共同的12L:12D明暗循环，灯光从06:00到18:00。鸡舍的照明采用40瓦的顶棚荧光灯泡，平均强度为200-300勒克斯。分别于每天8点、12点、16点、20点、24点、4点和8点采集1日龄、7日龄、16日龄、29日龄、65日龄和100日龄公鸡的血样。血浆中褪黑素浓度用放射免疫测定法测定。对照组（I和SP）的血浆褪黑素浓度表现出明显的日节律，在斑期中期达到峰值。在每一天的年龄，PX组的血浆褪黑素节律较小（约为黄斑中期对照水平的30%）。在BL bij:-ds中，血浆褪黑素在第1天显示出较小的日节律（在斑中期为对照水平的30%）。虽然随着年龄的增长，中期褪黑素水平有所上升，但16天后褪黑素水平与对照（I和SP）相同。PX-BL鸟类几乎没有日常节律。白色的里窝那小鸡。孵育后，部分雏鸡在连续白噪声环境下孵育以抵消噪声的影响，其余雏鸡在连续白噪声环境下孵育12h（6:00-18:00）。分别于每天8点、12点、16点、20点、24点、4点和8点采集1、7、13和24日龄鸡的松果体和血液样本。用放射免疫仪测定松果体中褪黑素含量和血浆中褪黑素浓度。然而，在连续白噪声和DD条件下，松果体中褪黑素含量和血浆中褪黑素浓度在实验过程中的任何时间都没有达到峰值。白噪声下DD 12h的雏鸟，松果体褪黑素含量在各日龄的6:00-18:00时段达到高峰。因此，这一结果表明噪声信号是形成节奏的潜在授时因子。少