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Studies on the Mechanism of Infection and Replication of Hepatitis B Virus

乙型肝炎病毒感染和复制机制的研究

基本信息

批准号：
63480159
负责人：
MATSUBARA Kenichi
金额：
$ 3.65万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480159/
关键词：
hepatitis B virus replication DNA polymerase core protein fusion polypeptide packaging viral nucleic acid packaging signal sequence PoC遺伝子 Pre S遺伝子 DNAトランスフェクション粒子形成融合タンパク B型肝炎ウイルス(HBV)表面抗原遺伝子ウイルス感染ウイルス粒子形成

项目摘要

In 1984 we have established a cell line that can be transfected by hepatitisB virus (HBV) DNA, and allows replication of this virus. Since then, many molecular biological studies with this virus have been done, but nothing has been understood as to the DNA polymerace (pol) production, and the mechanism where by viral nucleic acid is packaged into core. We have investigated into these two problems.1. We asked whether HBV pol is synthesized as a core-pol fusion poly-peptide, as suggested from the viral genome structure. For this purpose, a mutant genome that cannot initiate core protein, but can synthesize pol protein was constructed and used for transfection studies. The results showed that pol activity was made in the absence of core protein synthesis, proving that production of core-pol fusion protein is not a prerequisite for pol production.2. To obtain insights into packaging process of HBV genome nucleic acid into core particles, several deletion derivatives were constructed to see packaging ability. The results showed that the HBV genome carries a unique sequence to be recognized no packaging signal. This sequence was found not more than 75 nucleotide long.

1984年，我们建立了一个可以被乙型肝炎病毒（HBV）DNA转染的细胞系，并允许这种病毒复制。从那时起，已经对这种病毒进行了许多分子生物学研究，但对DNA聚合酶（pol）的产生以及病毒核酸包装到核心中的机制一无所知。我们已经调查了这两个问题。我们询问HBV pol是否合成为核心-pol融合多肽，如病毒基因组结构所示。为此，构建了不能启动核心蛋白但能合成pol蛋白的突变体基因组并用于转染研究。结果表明，pol活性是在没有核心蛋白合成的情况下产生的，证明核心-pol融合蛋白的产生不是pol产生的先决条件.为了深入了解HBV基因组核酸包装成核心颗粒的过程，构建了几种缺失衍生物来观察包装能力。结果表明，HBV基因组携带一个独特的序列被识别无包装信号。发现该序列不超过75个核苷酸长。