Studies with Promotion of Human Genome Programe

促进人类基因组计划的研究

• 批准号: 01102032
• 负责人: MATSUBARA Kenichi
• 金额: --
• 依托单位: Osaka University
• 依托单位国家: 日本
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01102032/
• 关键词: Human Genome; proposals; physical mapping; cDNA analyses; DNA technologies; informatics; co-ordination; ヒトゲノム解析; cDNAライブラリ-; 大量生物遺伝情報の科学; 各種生物ゲノム; 研究環境・研究体制; 将来計画策定

To promote the human genome programe in Japan, we set up five Committees for reviewing the state-of-the-art of the genome project in Japan, and for giving proposals needed for future development of human genome researches.To stimulate the genome-related researches that are now going on in Japan, the following group researches were organized ; (1) Physical mapping of human chromosomes, especially chromosomes 3, 11, 21. (2) cDNA analyses for understanding genes : develop methods for preparing high quality cDNA library, sequencing and mapping. (3) Improving techniques for DNA cleavage, separation of fragments. (4) Large scale informatics : Organizing researchers in this field for co-ordination, collaboration. (5) Analyses of model organisms for pioneering researches a complex genomes.

为了促进日本的人类基因组计划，我们成立了五个委员会，负责审查日本基因组计划的最新进展，并为人类基因组研究的未来发展提出所需的建议。为了促进日本正在进行的与基因组有关的研究，我们组织了以下小组研究：（1）人类染色体的物理作图，特别是3、11、21号染色体。(2)cDNA分析以了解基因：开发制备高质量cDNA文库、测序和作图的方法。(3)改进DNA切割、片段分离技术。(4)大规模信息学：组织该领域的研究人员进行协调和合作。(5)复杂基因组研究的模式生物分析。

项目成果

Ishihama,A.,Fujiyama,N.,Igarashi,K.and Ueshima,R.: "Structural and functional modulation of Escherichia coli RNA polymerase,In:Structure and Function of Nucleic Acid and Protins" Raven Press, New York. (1990)

Ishihama,A.、Fujiyama,N.、Igarashi,K. 和 Ueshima,R.：“大肠杆菌 RNA 聚合酶的结构和功能调节，见：核酸和蛋白质的结构和功能”Raven Press，纽约。

Misao Ohki and Cassandra L.Smith: "Traking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis" Nucleic Acid Res.17. 3479-3490 (1989)

Misao Ohki 和 Cassandra L.Smith：“通过脉冲场凝胶电泳追踪体内细菌 DNA 复制叉”Nucleic Acid Res.17。

Seto,Y.and Kanehisa,M.: "Repeat sequences of amino acids suggest the origin of protein Bull." Inst.Chem.Res.Kyoto Univ.66. 461-468 (1989)

Seto,Y. 和 Kanehisa,M.：“氨基酸的重复序列表明 Bull 蛋白的起源。”

松原謙一,他: "ヒト・ゲノムプログラムの推進について" 学術月報. 42. 226-231 (1989)

Kenichi Matsubara 等人：“人类基因组计划的推广”学术月刊报告 42. 226-231 (1989)。

Minoshima,S.,Kawasaki,K.,Fukuyama,R.,Maekuma,M.,Kuduh,J.and Shimizu,N.: "Isolation of Giant DNA Fragments From Flow-Sorted Human Chromosomes" Cytometry. 11. (1990)

Minoshima,S.、Kawasaki,K.、Fukuyama,R.、Maekuma,M.、Kuduh,J. 和 Shimizu,N.：“从流式分选人类染色体中分离巨型 DNA 片段”细胞计数。