Mechanism of Mammalian Fertilization and Treatment of Sterility

1. 人类精子的过度活化利用高速显微摄影技术分析了能性人类精子的悬浮运动特征。与其他哺乳动物一样，人类精子也存在超激活现象。用抗人纤维连接蛋白兔多克隆抗体处理精子，并连接用胶体金标记的二抗。用扫描电镜背散射电子成像模式观察精子。纤维连接蛋白定位于精子的赤道段。据报道，精卵融合从赤道段开始，对该区域纤维连接蛋白定位的认识表明，精子表面的纤维连接蛋白可能与受精有关。人精子顶体反应机理：宫颈粘液加速人精子获能。透明带诱导顶体反应，而卵积云不诱导顶体反应。哺乳动物精子核向原核发育的能力成熟的哺乳动物精子核在高温、酸、碱或高渗透压的作用下都能发育为原核。

1. Hyperactivation of human spermatozoaSuspensions of capacitating human spermatozoa were analyzed for movement characteristics using high-speed videomicrography. Hyperactivation exists human spermatozoa like other mammalian species.2. Participation of fibronectin in sperm-egg fusionSpermatozoa treated with anti-human fibronectin rabbit polyclonal antibody and were connected secondary antibody which was labelled with colloidal gold. Spermatozoa were observed with backscattered electron imaging mode of scanning electron microscopy. The localization of fibronectin was recognized in the equatorial segment of the spermatozoa. Sperm-egg fusion has reported to begin from the equatorial segment, and the recognition of the fibronectin localization in that region suggests that fibronectin on the surface of the spermatozoa is possibly related on the fertilization.3. Mechanism of acrosome reaction of human spermatozoaCervical mucus accelerates the capacitation of human spermatozoa. Zona pellucida induces the acrosome reaction, but cumulus oophorus has no ability to induce the acrosome reaction.4. The developing ability of mammalian sperm nuclei into pronucleiThe mature mammalian sperm nuclei treated with high temperature, acid, alkali or high osmotic pressure are capable of developing into pronuclei.