Mechanism of Mammalian Fertilization and Treatment of Sterility

哺乳动物的受精机制和不育治疗

• 批准号: 63480371
• 负责人: HOSHI Kazuhiko
• 金额: $ 3.2万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-63480371/
• 关键词: Spermatozoa; Hyperactivation; Acrosome reaction; Capacitation; Fibronectin; Zona pllucida; Cervical mucus; Male pronuclei; マイクロマニピュレ-ション; 体外受精; 初期胚; 先体反応; capacitation; 胚凍結; hyperactivation; 卵子; 受精卵凍結; 胚; フィブロネクチン

1. Hyperactivation of human spermatozoaSuspensions of capacitating human spermatozoa were analyzed for movement characteristics using high-speed videomicrography. Hyperactivation exists human spermatozoa like other mammalian species.2. Participation of fibronectin in sperm-egg fusionSpermatozoa treated with anti-human fibronectin rabbit polyclonal antibody and were connected secondary antibody which was labelled with colloidal gold. Spermatozoa were observed with backscattered electron imaging mode of scanning electron microscopy. The localization of fibronectin was recognized in the equatorial segment of the spermatozoa. Sperm-egg fusion has reported to begin from the equatorial segment, and the recognition of the fibronectin localization in that region suggests that fibronectin on the surface of the spermatozoa is possibly related on the fertilization.3. Mechanism of acrosome reaction of human spermatozoaCervical mucus accelerates the capacitation of human spermatozoa. Zona pellucida induces the acrosome reaction, but cumulus oophorus has no ability to induce the acrosome reaction.4. The developing ability of mammalian sperm nuclei into pronucleiThe mature mammalian sperm nuclei treated with high temperature, acid, alkali or high osmotic pressure are capable of developing into pronuclei.

1.人精子的超激活利用高速摄像技术分析人精子获能悬浮液的运动特性。与其他哺乳动物物种一样，人类精子也存在过度激活。纤维连接蛋白在精卵融合中的作用用抗人纤维连接蛋白兔多克隆抗体处理精子，并用胶体金标记法连接二抗。用扫描电子显微镜的背散射电子成像模式观察精子。纤维连接蛋白定位于精子赤道段。精子-卵子融合从赤道段开始，对该区域纤维连接蛋白定位的识别表明精子表面的纤维连接蛋白可能与受精有关。人精子顶体反应机制宫颈粘液促进人精子获能透明带可诱导顶体反应，而卵丘不能诱导顶体反应。哺乳动物精子核发育成原核的能力经高温、酸、碱或高渗透压处理的成熟哺乳动物精子核都能发育成原核。

项目成果

星 和彦他: "精子の形態的・機能的成熟" 日本受精着床学会雑誌. 8.

Kazuhiko Hoshi 等：“精子的形态和功能成熟”日本受精与着床学会杂志 8。

柳田薫・星和彦 他: 日本受精着床学会雑誌. 4. 70-74 (1988)

Kaoru Yanagita、Kazuhiko Hoshi 等：日本受精与着床学会杂志 4. 70-74 (1988)。

K.Hoshi,;K.Yanagida,et al: Tohoku J.Exp.Med.154. 47-56 (1988)

K.Hoshi，；K.Yanagida，等人：Tohoku J.Exp.Med.154。

星和彦・柳田薫 他: 日本受精着床学会雑誌. 6. 13-18 (1989)

Kazuhiko Hoshi、Kaoru Yanagita 等：日本受精与着床学会杂志，6. 13-18 (1989)。

Hoshi,K.,et al: "Changes in the motility pattern of human spermatozoa during in vitro incubation" Tohoku J.exp.Med.154. 47-56 (1988)

Hoshi,K.,et al：“体外孵化期间人类精子运动模式的变化”Tohoku J.exp.Med.154。